Keys to the table:
-[?/?]: Names of restriction enzyme used for digestion:; E, EcoRI; X, XbaI; S, SpeI; P, PstI
-The host cells used for transformation were competent JM109 E. coli cells
| Week 8 : July 6 – 10 |
| Monday 6 |
Transformation of pSB1C3-BBa_J23101, pSB1C3-BBa_J23106 and pSB1C3-BBa_J23117, and pSB1A2-BBa_I13504 into competent E. coli cells |
| Tuesday 7 |
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| Wednesday 8 |
Extraction of the plasmids pSB1C3-BBa_J23101, pSB1C3-BBa_J23106, pSB1C3-BBa_J23117 and pSB1A2-BBa_I13504 by mini-prep |
| Thursday 9 |
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| Friday 10 |
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| Week 9 : July 13 – 18 |
| Monday 13 |
Transformation of plasmid pSB1C3-BBa_I13504 into competent E. coli cells |
| Tuesday 14 |
Restriction digestion with [S/P] of the plasmid pSB1C3-BBa_J23101
Restriction digestion with [S/P] of the plasmid pSB1C3-BBa_J23106
Restriction digestion with [S/P] of the plasmid pSB1C3-BBa_J23117
Redo the transformation of plasmids pSB1C3-BBa_I20270 and pSB1C3-BBa_R0040
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| Wednesday 15 |
Extraction of plasmid pSB1C3-BBa_I13504Restriction digestion with [X/P] of the plasmid pSB1C3-BBa_I13504 |
| Thursday 16 |
Extraction of plasmids pSB1C3-BBa_I20270 and pSB1C3-BBa_R0040
Gel purification of the [X/P] digested I13504
Gel purification of July 14 [S/P] digested vectors pSB1C3-BBa_J23101, pSB1C3-BBa_J23106 and pSB1C3-BBa_J23117
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| Friday 17 |
Ligation of the [X/P] digested BBa_I13504 insert into [S/P] digested vector pSB1C3-BBa_J23101Ligation of the [X/P] digested BBa_I13504 insert into [S/P] digested vector pSB1C3-BBa_J23106Ligation of the [X/P] digested BBa_I13504 insert into [S/P] digested vector pSB1C3-BBa_J23117Transformation of the 3 ligation product |
| Saturday 18 |
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| Week 10 : July 20 – 25 |
| Monday 20 |
Redo the transformation of all July 17 ligation product |
| Tuesday 21 |
Redo the transformation of July 17 pSB1C3-BBa_J23101-I13504 and pSB1C3-BBa_J23117-BBa_I13504 ligation product
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| Wednesday 22 |
Extraction of plasmid pSB1C3-BBa_J23106-I13504Restriction analysis with [E/P] of the plasmid pSB1C3-BBa_J23106-BBa_I13504 |
| Thursday 23 |
Restriction analysis with [E/P] of the plasmids pSB1C3-BBa_J23101-I13504 and pSB1C3-BBa_J23117-I13504
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| Friday 24 |
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| Saturday 25 |
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| Week 11 : July 27 – August 1 |
| Monday 27 |
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| Tuesday 28 |
Checking of O.D A600, A511 - Positive control (I20270), negative control (R0040) and the J23101, J23106 and J23117
Redo the transformation of pSB1C3-BBa_J23117
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| Wednesday 29 |
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| Thursday 30 |
Extraction of the plasmid pSB1C3-BBa_J23117 Restriction digestion [S/P] of pSB1C3-BBa_J23117 Restriction digestion [X/P] of pSB1A2-BBa_I13504 Gel purification
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| Friday 31 |
Ligation of the [X/P] digested BBa_I13504 insert into the [S/P] digested pSB1C3-J23117Transformation |
| Saturday 1 |
Extraction of the plasmids pSB1C3-BBa_J23117 and pSB1A2_BBa_I13504Extraction of the plasmids pSB1C3-BBa_J23117 and pSB1A2_BBa_I13504 |
| Week 12 : August 3 – 8 |
| Monday 3 |
Redo the extraction of the plasmids pSB1C3-BBa_J23117 and pSB1A2_BBa_I13504Restriction analysis [E/P] |
| Tuesday 4 |
Redo the ligation of the [X/P] digested BBa_I13504 into the [S/P] digested pSB1C3-BBa_J23117
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