Template:Team:Groningen/CONTENT/PROTOCOLS/Restriction

Restriction

Component

Plasmid DNA

Unpurified Product

Genomic DNA

Water, nuclease free*

up to 20 μl

up to 20 μl

up to 50 μl

10x NEB 2.1 buffer

2 μl

2 μl**

5 μl

DNA

up to 1 μg

up to 0.2 μg

up to 5 μg

NEB Enzyme

0.5 μl

0.5 μl

0.5 μl

Total Volume

20 μl

20 μl

20 μl

Restriction.