Troubleshooting/Ligation

Troubleshooting

• Introduction
• General Tips and Tricks
1. Transformation
2. Ligation
3. Restriction Digest
• iGEM Protocols
• Answers
• Ask Your Questions

Ligation Troubleshooting

Now that you know you transformation efficiency and it's above 1 x 10⁸ CFU/µg DNA, we can work on other possible problems if you're still not getting great results from your cloning. This page is focused on common problems students have with ligations.

No colonies

• Press Kit
• Newsletter

• Upload Files
• Recent Changes
• Special Pages

• Requirements
• Calendar
• Meetups
• Giant Jamboree

• About
• Contact
• Sponsor
• Registry
• igem.org