Team:NCTU Formosa/Safety
This year Apollo designed a product mainly to detect antigens. Our users include scientific researchers, clinical scientist and so on. We believe these people all have the expertise, but we would like to once again remind everyone that our product contains E.coli. Therefore when applying our product, basic protective gear (lab coat, gloves and so on) are needed. Objects that come into contact with our product (for example: tips) need to be sterilized and thrown away, in case of non-natural gene outflow. We’ll hand our product to others after our safety procedure. Hence lowering the possibility of bio-contamination.
We designed a safety mechanism for our E.Cotector allowing it to be friendlier to users and environment. Our main objective is to maintain the fluorescence but kill the bacteria, which means that treated E.coli cannot grow in nutritious conditions such as LB.
Paraformaldehyde
At first, we treated our E.Cotector, displayed scFv(anti-EGFR) and green fluorescence protein(GFP), by paraformaldehyde. Paraformaldehyde is a chemical substance that is toxic for organism. It can fix the cell structure and all of the proteins.
According to the result, we found that paraformaldehyde-treated E.coli can grow in the LB broth at 37℃ incubated.
NCTU_Formosa APOllO
Engineering Building 6 EF455, 1001 University Road, Hsinchu 300, Taiwan, ROC.