Team:KU Leuven/InterLabStudy
Contact
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone n°: +32(0)16 32 73 19
Interlab study
Results
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Table 1 Fluorescence measurements
| LB + Cam | LB+Cam+Cells | D1 | D2 | D3 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 512 | 601 | 737 | 685 | 751 | 9425 | 9322 | 9737 | 22786 | 25895 | 25048 |
| 546 | 594 | 754 | 641 | 641 | 9669 | 9545 | 9876 | 23159 | 26460 | 24785 |
| 549 | 587 | 722 | 660 | 697 | 9407 | 9321 | 9677 | 22719 | 25931 | 24935 |
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Table 2 Absorbance measurements
| LB + Cam | LB+Cam+Cells | D1 | D2 | D3 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0.0434 | 0.2052 | 0.2174 | 0.1942 | 0.2201 | 0.2088 | 0.2082 | 0.2154 | 0.2139 | 0.2210 | 0.2218 |
| 0.0405 | 0.2000 | 0.2182 | 0.1974 | 0.1980 | 0.2108 | 0.2111 | 0.2123 | 0.2143 | 0.2179 | 0.2056 |
| 0.0379 | 0.2064 | 0.2141 | 0.1961 | 0.2036 | 0.2137 | 0.2117 | 0.2164 | 0.2202 | 0.2261 | 0.2083 |
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Table 3 Values for Biological replicates
| D1 | D2 | D3 | LB+Cam+Cells | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| T1 | T2 | T3 | T1 | T2 | T3 | T1 | T2 | T2 | ||
| B1 | 3390 | 3456 | 3372 | 45139 | 45868 | 44020 | 106526 | 108068 | 103174 | 2929 |
| B2 | 3527 | 3247 | 3366 | 44774 | 45216 | 44029 | 117172 | 121432 | 114688 | 2970 |
| B3 | 3399 | 3237 | 3426 | 45204 | 46519 | 44718 | 112931 | 120550 | 119707 | 2844 |
| Brep Avg | 3439 | 3313 | 3388 | 45039 | 45868 | 44256 | 112210 | 116683 | 112523 | |
| BRep SD | 270 | 101 | 162 | 189 | 532 | 327 | 4376 | 6102 | 6921 | |
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Table 4 Values for Technical replicates
| D1 | D2 | D3 | LB+Cam+ Cells | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| B1 | B2 | B3 | B1 | B2 | B3 | B1 | B2 | B3 | ||
| T1 | 3390 | 3527 | 3399 | 45139 | 44774 | 45204 | 106526 | 117172 | 112931 | 2929 |
| T2 | 3456 | 3247 | 3237 | 45868 | 45216 | 46519 | 108068 | 121432 | 120550 | 2970 |
| T3 | 3372 | 3366 | 3426 | 44020 | 44029 | 44718 | 103174 | 114688 | 119707 | 2844 |
| TRep Avg | 3406 | 3380 | 3354 | 45009 | 44673 | 45480 | 105923 | 117764 | 117729 | 2914 |
| TRep SD | 36 | 115 | 140 | 760 | 490 | 761 | 2043 | 2785 | 3410 | 52 |
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nunc nibh ligula, faucibus a elit id, egestas posuere est. Donec cursus enim non lacus dignissim, id vulputate leo tincidunt. Aliquam pretium luctus augue, nec tempor odio sagittis eu. Morbi rutrum suscipit purus a iaculis. Suspendisse in augue eget turpis volutpat bibendum. Vivamus vel libero ac velit pulvinar auctor in eu nunc. Duis urna lectus, cursus ac tempor sed, lobortis id tellus.
Table 5 Standard graph
| Fluorescein standard | ||||||
|---|---|---|---|---|---|---|
| concentration (ng/mL) | F1 | F2 | F3 | average F | average F' | SD |
| 0 | 9 | 13 | 12 | 11 | 0 | 1.699673171 |
| 10 | 1140 | 1168 | 1151 | 1153 | 1142 | 11.5181017 |
| 25 | 2543 | 2600 | 2631 | 2591 | 2580 | 36.4447832 |
| 50 | 4822 | 5097 | 5154 | 5024 | 5013 | 144.9513328 |
| 125 | 12879 | 12902 | 12931 | 12904 | 12893 | 21.27596453 |
| 250 | 25494 | 25761 | 25765 | 25673 | 25662 | 126.8183303 |
| 375 | 37474 | 37505 | 37899 | 37626 | 37615 | 193.4545597 |
| 500 | 46698 | 49051 | 48798 | 48182 | 48171 | 1054.652023 |
Figure 1
The designed circuit
Table 6 Extrapolation of GFP concentration using fluorescence measurements and fluorescein standard
| D1 | D2 | D3 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Brep Avg | 3439 | 3313 | 3388 | 45039 | 45868 | 44256 | 112210 | 116683 | 112523 |
| (Brep Avg) - (LB+cam+cells avg) | 525 | 399 | 474 | 42125 | 42954 | 41342 | 109296 | 113769 | 109609 |
| Concentration (ng/mL) | 1.929 | 0.639 | 1.407 | 427.88 | 436.36 | 419.88 | 1115.64 | 1161.44 | 1118.85 |
| TRep Avg | 3406 | 3380 | 3354 | 45009 | 44673 | 45480 | 105923 | 117764 | 117729 |
| (Trep avg) - (LB+cam+cellsavg) | 492 | 466 | 440 | 42095 | 41759 | 42566 | 103009 | 114850 | 114815 |
| Concentration (ng/mL) | 1.591 | 1.325 | 1.059 | 427.56 | 424.12 | 432.39 | 1051.27 | 1172.51 | 1172.15 |
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