Team:Aalto-Helsinki/InterLab
The following devices were created in a backbone pSB1C3: ⚫ J23101 + I13504 (B0034-E0040-B0015) ⚫ J23106 + I13504 (B0034-E0040-B0015). However, constructing a device J23117 + I13504 (B0034-E0040-B0015) wasn't successful due to the time limit and limited ligation times. More details available at the lab book(make link about this). The device sizes were analyzed with restriction mapping which results can be seen in Figure 1.
Protocols for the restriction, ligation and transformation of BioBricks can be found here (Add link).
Sample |
J23117 + I13504 |
J23101 + I13504 |
J23151 |
Empty backbone |
Without backbone |
Blank |
1 |
D1 |
D2 |
C1 |
C2 |
C3 |
B |
1 |
D1 |
D2 |
C1 |
C2 |
C3 |
B |
1 |
D1 |
D2 |
C1 |
C2 |
C3 |
B |