Team:UCLA/Notebook/Honeybee Silk/16 May 2015

Plating Bacteria for Honeybee Silk Protein Expression

I am plating these bacterial glycerol stocks in order to get colonies, which I will inoculate in order to express and purify our honeybee silk protein.

• Plated cells on choramphenicol agar plates using glycerol stocks of regulatory elements + silk#3 coding
  • Out of the three tubes of glycerol stocks, I used tube #1 on plate #1 and tube #2 on plate #2.
  • I spread the bacteria using a bacterial streaker instead of beads