Team:UCLA/Notebook/Honeybee Silk/19 May 2015

5/19

Cell Harvesting

• Cells were grown up in 250 ml of LB medium and induced with IPTG. See 5/18 for details.
• The Cells were split up into 5 pre weighed 50 ml falcon tubes, with 45 ml of cells in each tube.
  • I should have taken the OD 600 at this point, but I forgot to.
• Mass Balanced the tubes and added a water tube, and spun down the cells at 5300 rpm at 4 C for 15 min. to pellet the cells.
• Decanted the supernatant.
• Weighed the pellets
  • In total, the wet weight of all the pellets across all 5 falcon tubes was 2.42 grams, approximately 0.5 grams per 45 ml of culture.
• Froze down the pellets in the falcon tubes at -80C