Team:Nankai/Collaborations

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Collaboration

 

As the famous Chinese proverb goes,"Union is strength". It adequately shows the importance of collaboration.During the past years, our school has been in a good cooperation with Tianjin University, which is our intimate friend.And so is our igem team. This year we have discussed lots of things about synthetic biology together and both benefitted a lot from the proceedings. Besides, on the basis of this, this year our team have make up a new platform which is named"iShare". It is a part for all the igem teams to get what they need. To gather the information, we have also searched for much help from many other teams including WHU、ZJU and etc. And till now, our iShare part is already prepared to be put into use.

Collaboration

 

I. Communication with our neighbor TJU

 

The Lotus Festival held at TJU

 

At the eary April, when the lotus is the most exuberant in the school, TJU's igem team held a lotus festival to popularize the knowledge of synthetic biology. As their neighbor and cooperative partner, our igem team are invited. During the festival, we had a good time with them. We not only help them to the posters but also communicated with their team members. And at last, acquainted with each other's project, we reached an agreement to help each other's team if needed.

 

The front door of TJU.

Their poster.

Group photo at TJU.

 

Supporting the statistics of eukaryotic expression

This year, the igem team of Tianjin Universitiy is working on a special hydrophobin HGFII. Apart from the gene of IGFII, they also own the gene of HGFI. However, what they did is the prokaryotic expression in escherichia coli, so they lacked the conditions in eucells to test this gene. Coincidengly, there is a professor in our school which is working on the fermentation of HGFI. Hearing this, we visited his lab and successfully obtained these statistics. And finally, We support these data for their team.

The result of the western blotting

On the left are the real HPLC（High Performance Liquid Chromatography) results. From these pictures we can see that in the pure products the peak appeared at around 30 minutes while at the fermentation broth the peak appeared at aound 33 minutes.

The HPLC result of the pure HFGI

The HPLC result of HFGI in the fermentation broth

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Nankai IGEM Team 2015