Team:Technion Israel/Lab Notebook/Protocols

Project Lab Notebook Judging Criteria Human Practices Team

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Overview Bacillus E.Coli NADPH Comb Modeling

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• Gel Extraction

• Sigma-Aldrich Miniprep

• Phusion PCR

• Electroporation

  E.coli

  B.Subtilis

• Transformation

  E.coli

  B.Subtilis

• Colony PCR

  B. Subtilis

• Preparing Competent E.coli

• DNA Gel Electrophoresis

• Preparation of DNA for Sequencing

• Comparison of Lysis Procedures

• Primer Dilution

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