Team:NAIT Edmonton/Practices

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Human Practices

What type of work does it take to get to the Jamboree? What do you need to know? We reached out to teams across the world to get their perspective on their iGEM journey and compiled the information so that future teams can draw from our collective experiences and learn!

Theorizing our Sequences

Literature has shown certain proteins inherently stain in colour.

Looked up characteristics of said proteins

Isolated and identified the unique characteristics of said proteins so that we can manually write our own sequences and generate custom proteins.

Writing our Sequences

Went down to base pair level and wrote out our sequences with the defining characteristics identified in literature.

Ordered custom gBlocks from Integrated DNA Technologies (IDT).

PCR

Make a PCR Master Mix

Set up individual reactions

Run PCR
(click for specific conditions)

Run agarose gel electrophoresis to confirm that PCR was successful

QIAquick PCR Purification

Digestion and Ligation

Literature has shown certain proteins inherently stain in colour.

Step 1: Blah blah blah, do this.

Step 1: Blah blah blah, do this.

Transforming Bacteria

Literature has shown certain proteins inherently stain in colour.

Step 1: Blah blah blah, do this.

Step 1: Blah blah blah, do this.

Validating the Transformation

Literature has shown certain proteins inherently stain in colour.

Step 1: Blah blah blah, do this.

Step 1: Blah blah blah, do this.

Protein Isolation and Purification

Literature has shown certain proteins inherently stain in colour.

Step 1: Blah blah blah, do this.

Step 1: Blah blah blah, do this.

SDS PAGE and Silver Staining

Make PA Gel

Run Electrophoresis

Silver Stain Plus