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Lab Safety

Safety Level

The TU Eindhoven has multiple lab spaces with different Safety Levels. As an iGEM team, access to one of the labs with BioSafety Level 1 was gained,

Within Biosafety Level 1, people are allowed to work with microorganisms which cannot cause any diseases or harm to humans.

for us the ML-I laboratory. In this kind of lab most work involved recombinant expression of protein in E. coli or yeast expression systems, and the application of bacteriophages for phage display.
In the ML-II laboratory, with BioSafety Level 2

Within Biosafety Level 2, people are allowed to work with microorganisms which do not easily cause a disease. It also includes microorganisms which cause a disease for which a vaccine or cure is known. The rules to enter a Biosafety Level 2 laboratory are stricter.

, the work typically involves the use of immortalized cell lines, but also tissues or primary cells from animals or humans. In the ML-II also takes place the genetic modifications of eukaryotic cell lines for addressing localization and interaction of transfected proteins.
During our project a lab with BioSafety Level 1 is sufficient, because only work with the E. coli chassis was preformed, which is a risk group 1 organism.

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Safety Training

After a safety training and tour through an ML-I laboratory of TU Eindhoven by the lab manager Peggy de Graaf, with a closing of signing an agreement, the team gained access to the lab, located in the Helix building on the TU Eindhoven campus. During the training the topics were mostly about the equipment, the biosafety rules in the Netherlands. (which follows the Cartegena- and Europeanprotocols.

The most valid Biosafety rules in our lab are:

• Keep windows and doors shut.