Team:OUC-China

Team:OUC-China Team: OUC-China

Platform for Electonmagnetic Control

HOW DOES IT WORK?

This summer we are trying to design a platform for remote regular of gene expression by electromagnetic signal.

STEP 1

The platform is mainly constructed by magnetic receiver and regulator.

STEP 2

Induced by electromagnetic fidle, magnetic receiver would be heated.

STEP 3

In response to change in temprature, thermosensitive regulator would activate GFP expression.

Magnetic Receiver

For magnetic receiver, we choose ferritin, the iron-storage protein in organisms, which could synthesis terric***core in its hollow protein shell. When exposed to electromagnetic field, the ferritin oxihydroxide core would be heated.

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Thermosensitive Regulator

For thermosensitive regulator, we choose RNA thermosensitive and constructed a thermosensitive T7 RNA polymerase. RNA thermometer is a structured RNA which could expose RBS only when heated. The thermosensitive T7 RNA polymerase is a normal T7 RNA polymerase at the selected locus of which was inserted by a temprature-sensitive intein, which could self-splice at specific temperature.

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