Team:Birkbeck/InterLab Study/fluorescence ANOVA

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Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




Multiple ComparisonsMultiple Comparisons of Fluorescence
Fluorescence
Tukey HSD
(I) Organism (J) Organism Mean Difference (I-J) Std. Error Sig. 95% Confidence Interval
Lower Bound Upper Bound
E. coli DH5 alpha E. coli DH5 alpha + P1 -69557.333* 2150.035 .000 -75938.41 -63176.25
E. coli DH5 alpha + P2 -35413.889* 2150.035 .000 -41794.97 -29032.81
E. coli DH5 alpha + P3 -4798.889 2150.035 .243 -11179.97 1582.19
E. coli DH5 alpha Positive control -22105.556* 2150.035 .000 -28486.63 -15724.48
E. coli DH5 alpha negative control -1705.556 2150.035 .967 -8086.63 4675.52
E. coli DH5 alpha + P1 E. coli DH5 alpha 69557.333* 2150.035 .000 63176.25 75938.41
E. coli DH5 alpha + P2 34143.444* 2150.035 .000 27762.37 40524.52
E. coli DH5 alpha + P3 64758.444* 2150.035 .000 58377.37 71139.52
E. coli DH5 alpha Positive control 47451.778* 2150.035 .000 41070.70 53832.86
E. coli DH5 alpha negative control 67851.778* 2150.035 .000 61470.70 74232.86
E. coli DH5 alpha + P2 E. coli DH5 alpha 35413.889* 2150.035 .000 29032.81 41794.97
E. coli DH5 alpha + P1 -34143.444* 2150.035 .000 -40524.52 -27762.37
E. coli DH5 alpha + P3 30615.000* 2150.035 .000 24233.92 36996.08
E. coli DH5 alpha Positive control 13308.333* 2150.035 .000 6927.25 19689.41
E. coli DH5 alpha negative control 33708.333* 2150.035 .000 27327.25 40089.41
E. coli DH5 alpha + P3 E. coli DH5 alpha 4798.889 2150.035 .243 -1582.19 11179.97
E. coli DH5 alpha + P1 -64758.444* 2150.035 .000 -71139.52 -58377.37
E. coli DH5 alpha + P2 -30615.000* 2150.035 .000 -36996.08 -24233.92
E. coli DH5 alpha Positive control -17306.667* 2150.035 .000 -23687.75 -10925.59
E. coli DH5 alpha negative control 3093.333 2150.035 .704 -3287.75 9474.41
E. coli DH5 alpha Positive control E. coli DH5 alpha 22105.556* 2150.035 .000 15724.48 28486.63
E. coli DH5 alpha + P1 -47451.778* 2150.035 .000 -53832.86 -41070.70
E. coli DH5 alpha + P2 -13308.333* 2150.035 .000 -19689.41 -6927.25
E. coli DH5 alpha + P3 17306.667* 2150.035 .000 10925.59 23687.75
E. coli DH5 alpha negative control 20400.000* 2150.035 .000 14018.92 26781.08
E. coli DH5 alpha negative control E. coli DH5 alpha 1705.556 2150.035 .967 -4675.52 8086.63
E. coli DH5 alpha + P1 -67851.778* 2150.035 .000 -74232.86 -61470.70
E. coli DH5 alpha + P2 -33708.333* 2150.035 .000 -40089.41 -27327.25
E. coli DH5 alpha + P3 -3093.333 2150.035 .704 -9474.41 3287.75
E. coli DH5 alpha Positive control -20400.000* 2150.035 .000 -26781.08 -14018.92
*. The mean difference is significant at the 0.05 level.