Team:Birkbeck/InterLab Study/fluorescence ANOVA ungrouped

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Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




Multiple ComparisonsMultiple Comparisons of Fluorescence (ungrouped)
Fluorescence
Tukey HSD
(I) GroupingTtest (J) GroupingTtest Mean Difference (I-J) Std. Error Sig. 95% Confidence Interval
Lower Bound Upper Bound
E. coli DH5 alpha (first triplicate) E. coli DH5 alpha (second triplicate) -332.000 3563.444 1.000 -13696.17 13032.17
E. coli DH5 alpha (third triplicate) 13.000 3563.444 1.000 -13351.17 13377.17
E. coli DH5 alpha P1 (first triplicate) -75226.000* 3563.444 .000 -88590.17 -61861.83
E. coli DH5 alpha P1 (second triplicate) -67646.000* 3563.444 .000 -81010.17 -54281.83
E. coli DH5 alpha P1 (third triplicate) -66119.000* 3563.444 .000 -79483.17 -52754.83
E. coli DH5 alpha P2 (first triplicate) -30450.000* 3563.444 .000 -43814.17 -17085.83
E. coli DH5 alpha P2 (second triplicate) -40893.667* 3563.444 .000 -54257.83 -27529.50
E. coli DH5 alpha P2 (third triplicate) -35217.000* 3563.444 .000 -48581.17 -21852.83
E. coli DH5 alpha P3 (first triplicate) -4449.667 3563.444 .998 -17813.83 8914.50
E. coli DH5 alpha P3 (second triplicate) -5821.667 3563.444 .967 -19185.83 7542.50
E. coli DH5 alpha P3 (third triplicate) -4444.333 3563.444 .998 -17808.50 8919.83
E. coli DH5 alpha Positive control (first triplicate) -21639.333* 3563.444 .000 -35003.50 -8275.17
E. coli DH5 alpha Positive control (second triplicate) -22461.333* 3563.444 .000 -35825.50 -9097.17
E. coli DH5 alpha Positive control (third triplicate) -22535.000* 3563.444 .000 -35899.17 -9170.83
E. coli DH5 alpha negative control (first triplicate) -2080.000 3563.444 1.000 -15444.17 11284.17
E. coli DH5 alpha negative control (second triplicate) -1480.667 3563.444 1.000 -14844.83 11883.50
E. coli DH5 alpha negative control (third triplicate) -1875.000 3563.444 1.000 -15239.17 11489.17
E. coli DH5 alpha (second triplicate) E. coli DH5 alpha (first triplicate) 332.000 3563.444 1.000 -13032.17 13696.17
E. coli DH5 alpha (third triplicate) 345.000 3563.444 1.000 -13019.17 13709.17
E. coli DH5 alpha P1 (first triplicate) -74894.000* 3563.444 .000 -88258.17 -61529.83
E. coli DH5 alpha P1 (second triplicate) -67314.000* 3563.444 .000 -80678.17 -53949.83
E. coli DH5 alpha P1 (third triplicate) -65787.000* 3563.444 .000 -79151.17 -52422.83
E. coli DH5 alpha P2 (first triplicate) -30118.000* 3563.444 .000 -43482.17 -16753.83
E. coli DH5 alpha P2 (second triplicate) -40561.667* 3563.444 .000 -53925.83 -27197.50
E. coli DH5 alpha P2 (third triplicate) -34885.000* 3563.444 .000 -48249.17 -21520.83
E. coli DH5 alpha P3 (first triplicate) -4117.667 3563.444 .999 -17481.83 9246.50
E. coli DH5 alpha P3 (second triplicate) -5489.667 3563.444 .981 -18853.83 7874.50
E. coli DH5 alpha P3 (third triplicate) -4112.333 3563.444 .999 -17476.50 9251.83
E. coli DH5 alpha Positive control (first triplicate) -21307.333* 3563.444 .000 -34671.50 -7943.17
E. coli DH5 alpha Positive control (second triplicate) -22129.333* 3563.444 .000 -35493.50 -8765.17
E. coli DH5 alpha Positive control (third triplicate) -22203.000* 3563.444 .000 -35567.17 -8838.83
E. coli DH5 alpha negative control (first triplicate) -1748.000 3563.444 1.000 -15112.17 11616.17
E. coli DH5 alpha negative control (second triplicate) -1148.667 3563.444 1.000 -14512.83 12215.50
E. coli DH5 alpha negative control (third triplicate) -1543.000 3563.444 1.000 -14907.17 11821.17
E. coli DH5 alpha (third triplicate) E. coli DH5 alpha (first triplicate) -13.000 3563.444 1.000 -13377.17 13351.17
E. coli DH5 alpha (second triplicate) -345.000 3563.444 1.000 -13709.17 13019.17
E. coli DH5 alpha P1 (first triplicate) -75239.000* 3563.444 .000 -88603.17 -61874.83
E. coli DH5 alpha P1 (second triplicate) -67659.000* 3563.444 .000 -81023.17 -54294.83
E. coli DH5 alpha P1 (third triplicate) -66132.000* 3563.444 .000 -79496.17 -52767.83
E. coli DH5 alpha P2 (first triplicate) -30463.000* 3563.444 .000 -43827.17 -17098.83
E. coli DH5 alpha P2 (second triplicate) -40906.667* 3563.444 .000 -54270.83 -27542.50
E. coli DH5 alpha P2 (third triplicate) -35230.000* 3563.444 .000 -48594.17 -21865.83
E. coli DH5 alpha P3 (first triplicate) -4462.667 3563.444 .998 -17826.83 8901.50
E. coli DH5 alpha P3 (second triplicate) -5834.667 3563.444 .967 -19198.83 7529.50
E. coli DH5 alpha P3 (third triplicate) -4457.333 3563.444 .998 -17821.50 8906.83
E. coli DH5 alpha Positive control (first triplicate) -21652.333* 3563.444 .000 -35016.50 -8288.17
E. coli DH5 alpha Positive control (second triplicate) -22474.333* 3563.444 .000 -35838.50 -9110.17
E. coli DH5 alpha Positive control (third triplicate) -22548.000* 3563.444 .000 -35912.17 -9183.83
E. coli DH5 alpha negative control (first triplicate) -2093.000 3563.444 1.000 -15457.17 11271.17
E. coli DH5 alpha negative control (second triplicate) -1493.667 3563.444 1.000 -14857.83 11870.50
E. coli DH5 alpha negative control (third triplicate) -1888.000 3563.444 1.000 -15252.17 11476.17
E. coli DH5 alpha P1 (first triplicate) E. coli DH5 alpha (first triplicate) 75226.000* 3563.444 .000 61861.83 88590.17
E. coli DH5 alpha (second triplicate) 74894.000* 3563.444 .000 61529.83 88258.17
E. coli DH5 alpha (third triplicate) 75239.000* 3563.444 .000 61874.83 88603.17
E. coli DH5 alpha P1 (second triplicate) 7580.000 3563.444 .784 -5784.17 20944.17
E. coli DH5 alpha P1 (third triplicate) 9107.000 3563.444 .508 -4257.17 22471.17
E. coli DH5 alpha P2 (first triplicate) 44776.000* 3563.444 .000 31411.83 58140.17
E. coli DH5 alpha P2 (second triplicate) 34332.333* 3563.444 .000 20968.17 47696.50
E. coli DH5 alpha P2 (third triplicate) 40009.000* 3563.444 .000 26644.83 53373.17
E. coli DH5 alpha P3 (first triplicate) 70776.333* 3563.444 .000 57412.17 84140.50
E. coli DH5 alpha P3 (second triplicate) 69404.333* 3563.444 .000 56040.17 82768.50
E. coli DH5 alpha P3 (third triplicate) 70781.667* 3563.444 .000 57417.50 84145.83
E. coli DH5 alpha Positive control (first triplicate) 53586.667* 3563.444 .000 40222.50 66950.83
E. coli DH5 alpha Positive control (second triplicate) 52764.667* 3563.444 .000 39400.50 66128.83
E. coli DH5 alpha Positive control (third triplicate) 52691.000* 3563.444 .000 39326.83 66055.17
E. coli DH5 alpha negative control (first triplicate) 73146.000* 3563.444 .000 59781.83 86510.17
E. coli DH5 alpha negative control (second triplicate) 73745.333* 3563.444 .000 60381.17 87109.50
E. coli DH5 alpha negative control (third triplicate) 73351.000* 3563.444 .000 59986.83 86715.17
E. coli DH5 alpha P1 (second triplicate) E. coli DH5 alpha (first triplicate) 67646.000* 3563.444 .000 54281.83 81010.17
E. coli DH5 alpha (second triplicate) 67314.000* 3563.444 .000 53949.83 80678.17
E. coli DH5 alpha (third triplicate) 67659.000* 3563.444 .000 54294.83 81023.17
E. coli DH5 alpha P1 (first triplicate) -7580.000 3563.444 .784 -20944.17 5784.17
E. coli DH5 alpha P1 (third triplicate) 1527.000 3563.444 1.000 -11837.17 14891.17
E. coli DH5 alpha P2 (first triplicate) 37196.000* 3563.444 .000 23831.83 50560.17
E. coli DH5 alpha P2 (second triplicate) 26752.333* 3563.444 .000 13388.17 40116.50
E. coli DH5 alpha P2 (third triplicate) 32429.000* 3563.444 .000 19064.83 45793.17
E. coli DH5 alpha P3 (first triplicate) 63196.333* 3563.444 .000 49832.17 76560.50
E. coli DH5 alpha P3 (second triplicate) 61824.333* 3563.444 .000 48460.17 75188.50
E. coli DH5 alpha P3 (third triplicate) 63201.667* 3563.444 .000 49837.50 76565.83
E. coli DH5 alpha Positive control (first triplicate) 46006.667* 3563.444 .000 32642.50 59370.83
E. coli DH5 alpha Positive control (second triplicate) 45184.667* 3563.444 .000 31820.50 58548.83
E. coli DH5 alpha Positive control (third triplicate) 45111.000* 3563.444 .000 31746.83 58475.17
E. coli DH5 alpha negative control (first triplicate) 65566.000* 3563.444 .000 52201.83 78930.17
E. coli DH5 alpha negative control (second triplicate) 66165.333* 3563.444 .000 52801.17 79529.50
E. coli DH5 alpha negative control (third triplicate) 65771.000* 3563.444 .000 52406.83 79135.17
E. coli DH5 alpha P1 (third triplicate) E. coli DH5 alpha (first triplicate) 66119.000* 3563.444 .000 52754.83 79483.17
E. coli DH5 alpha (second triplicate) 65787.000* 3563.444 .000 52422.83 79151.17
E. coli DH5 alpha (third triplicate) 66132.000* 3563.444 .000 52767.83 79496.17
E. coli DH5 alpha P1 (first triplicate) -9107.000 3563.444 .508 -22471.17 4257.17
E. coli DH5 alpha P1 (second triplicate) -1527.000 3563.444 1.000 -14891.17 11837.17
E. coli DH5 alpha P2 (first triplicate) 35669.000* 3563.444 .000 22304.83 49033.17
E. coli DH5 alpha P2 (second triplicate) 25225.333* 3563.444 .000 11861.17 38589.50
E. coli DH5 alpha P2 (third triplicate) 30902.000* 3563.444 .000 17537.83 44266.17
E. coli DH5 alpha P3 (first triplicate) 61669.333* 3563.444 .000 48305.17 75033.50
E. coli DH5 alpha P3 (second triplicate) 60297.333* 3563.444 .000 46933.17 73661.50
E. coli DH5 alpha P3 (third triplicate) 61674.667* 3563.444 .000 48310.50 75038.83
E. coli DH5 alpha Positive control (first triplicate) 44479.667* 3563.444 .000 31115.50 57843.83
E. coli DH5 alpha Positive control (second triplicate) 43657.667* 3563.444 .000 30293.50 57021.83
E. coli DH5 alpha Positive control (third triplicate) 43584.000* 3563.444 .000 30219.83 56948.17
E. coli DH5 alpha negative control (first triplicate) 64039.000* 3563.444 .000 50674.83 77403.17
E. coli DH5 alpha negative control (second triplicate) 64638.333* 3563.444 .000 51274.17 78002.50
E. coli DH5 alpha negative control (third triplicate) 64244.000* 3563.444 .000 50879.83 77608.17
E. coli DH5 alpha P2 (first triplicate) E. coli DH5 alpha (first triplicate) 30450.000* 3563.444 .000 17085.83 43814.17
E. coli DH5 alpha (second triplicate) 30118.000* 3563.444 .000 16753.83 43482.17
E. coli DH5 alpha (third triplicate) 30463.000* 3563.444 .000 17098.83 43827.17
E. coli DH5 alpha P1 (first triplicate) -44776.000* 3563.444 .000 -58140.17 -31411.83
E. coli DH5 alpha P1 (second triplicate) -37196.000* 3563.444 .000 -50560.17 -23831.83
E. coli DH5 alpha P1 (third triplicate) -35669.000* 3563.444 .000 -49033.17 -22304.83
E. coli DH5 alpha P2 (second triplicate) -10443.667 3563.444 .285 -23807.83 2920.50
E. coli DH5 alpha P2 (third triplicate) -4767.000 3563.444 .995 -18131.17 8597.17
E. coli DH5 alpha P3 (first triplicate) 26000.333* 3563.444 .000 12636.17 39364.50
E. coli DH5 alpha P3 (second triplicate) 24628.333* 3563.444 .000 11264.17 37992.50
E. coli DH5 alpha P3 (third triplicate) 26005.667* 3563.444 .000 12641.50 39369.83
E. coli DH5 alpha Positive control (first triplicate) 8810.667 3563.444 .563 -4553.50 22174.83
E. coli DH5 alpha Positive control (second triplicate) 7988.667 3563.444 .715 -5375.50 21352.83
E. coli DH5 alpha Positive control (third triplicate) 7915.000 3563.444 .728 -5449.17 21279.17
E. coli DH5 alpha negative control (first triplicate) 28370.000* 3563.444 .000 15005.83 41734.17
E. coli DH5 alpha negative control (second triplicate) 28969.333* 3563.444 .000 15605.17 42333.50
E. coli DH5 alpha negative control (third triplicate) 28575.000* 3563.444 .000 15210.83 41939.17
E. coli DH5 alpha P2 (second triplicate) E. coli DH5 alpha (first triplicate) 40893.667* 3563.444 .000 27529.50 54257.83
E. coli DH5 alpha (second triplicate) 40561.667* 3563.444 .000 27197.50 53925.83
E. coli DH5 alpha (third triplicate) 40906.667* 3563.444 .000 27542.50 54270.83
E. coli DH5 alpha P1 (first triplicate) -34332.333* 3563.444 .000 -47696.50 -20968.17
E. coli DH5 alpha P1 (second triplicate) -26752.333* 3563.444 .000 -40116.50 -13388.17
E. coli DH5 alpha P1 (third triplicate) -25225.333* 3563.444 .000 -38589.50 -11861.17
E. coli DH5 alpha P2 (first triplicate) 10443.667 3563.444 .285 -2920.50 23807.83
E. coli DH5 alpha P2 (third triplicate) 5676.667 3563.444 .974 -7687.50 19040.83
E. coli DH5 alpha P3 (first triplicate) 36444.000* 3563.444 .000 23079.83 49808.17
E. coli DH5 alpha P3 (second triplicate) 35072.000* 3563.444 .000 21707.83 48436.17
E. coli DH5 alpha P3 (third triplicate) 36449.333* 3563.444 .000 23085.17 49813.50
E. coli DH5 alpha Positive control (first triplicate) 19254.333* 3563.444 .001 5890.17 32618.50
E. coli DH5 alpha Positive control (second triplicate) 18432.333* 3563.444 .001 5068.17 31796.50
E. coli DH5 alpha Positive control (third triplicate) 18358.667* 3563.444 .001 4994.50 31722.83
E. coli DH5 alpha negative control (first triplicate) 38813.667* 3563.444 .000 25449.50 52177.83
E. coli DH5 alpha negative control (second triplicate) 39413.000* 3563.444 .000 26048.83 52777.17
E. coli DH5 alpha negative control (third triplicate) 39018.667* 3563.444 .000 25654.50 52382.83
E. coli DH5 alpha P2 (third triplicate) E. coli DH5 alpha (first triplicate) 35217.000* 3563.444 .000 21852.83 48581.17
E. coli DH5 alpha (second triplicate) 34885.000* 3563.444 .000 21520.83 48249.17
E. coli DH5 alpha (third triplicate) 35230.000* 3563.444 .000 21865.83 48594.17
E. coli DH5 alpha P1 (first triplicate) -40009.000* 3563.444 .000 -53373.17 -26644.83
E. coli DH5 alpha P1 (second triplicate) -32429.000* 3563.444 .000 -45793.17 -19064.83
E. coli DH5 alpha P1 (third triplicate) -30902.000* 3563.444 .000 -44266.17 -17537.83
E. coli DH5 alpha P2 (first triplicate) 4767.000 3563.444 .995 -8597.17 18131.17
E. coli DH5 alpha P2 (second triplicate) -5676.667 3563.444 .974 -19040.83 7687.50
E. coli DH5 alpha P3 (first triplicate) 30767.333* 3563.444 .000 17403.17 44131.50
E. coli DH5 alpha P3 (second triplicate) 29395.333* 3563.444 .000 16031.17 42759.50
E. coli DH5 alpha P3 (third triplicate) 30772.667* 3563.444 .000 17408.50 44136.83
E. coli DH5 alpha Positive control (first triplicate) 13577.667* 3563.444 .043 213.50 26941.83
E. coli DH5 alpha Positive control (second triplicate) 12755.667 3563.444 .075 -608.50 26119.83
E. coli DH5 alpha Positive control (third triplicate) 12682.000 3563.444 .079 -682.17 26046.17
E. coli DH5 alpha negative control (first triplicate) 33137.000* 3563.444 .000 19772.83 46501.17
E. coli DH5 alpha negative control (second triplicate) 33736.333* 3563.444 .000 20372.17 47100.50
E. coli DH5 alpha negative control (third triplicate) 33342.000* 3563.444 .000 19977.83 46706.17
E. coli DH5 alpha P3 (first triplicate) E. coli DH5 alpha (first triplicate) 4449.667 3563.444 .998 -8914.50 17813.83
E. coli DH5 alpha (second triplicate) 4117.667 3563.444 .999 -9246.50 17481.83
E. coli DH5 alpha (third triplicate) 4462.667 3563.444 .998 -8901.50 17826.83
E. coli DH5 alpha P1 (first triplicate) -70776.333* 3563.444 .000 -84140.50 -57412.17
E. coli DH5 alpha P1 (second triplicate) -63196.333* 3563.444 .000 -76560.50 -49832.17
E. coli DH5 alpha P1 (third triplicate) -61669.333* 3563.444 .000 -75033.50 -48305.17
E. coli DH5 alpha P2 (first triplicate) -26000.333* 3563.444 .000 -39364.50 -12636.17
E. coli DH5 alpha P2 (second triplicate) -36444.000* 3563.444 .000 -49808.17 -23079.83
E. coli DH5 alpha P2 (third triplicate) -30767.333* 3563.444 .000 -44131.50 -17403.17
E. coli DH5 alpha P3 (second triplicate) -1372.000 3563.444 1.000 -14736.17 11992.17
E. coli DH5 alpha P3 (third triplicate) 5.333 3563.444 1.000 -13358.83 13369.50
E. coli DH5 alpha Positive control (first triplicate) -17189.667* 3563.444 .003 -30553.83 -3825.50
E. coli DH5 alpha Positive control (second triplicate) -18011.667* 3563.444 .001 -31375.83 -4647.50
E. coli DH5 alpha Positive control (third triplicate) -18085.333* 3563.444 .001 -31449.50 -4721.17
E. coli DH5 alpha negative control (first triplicate) 2369.667 3563.444 1.000 -10994.50 15733.83
E. coli DH5 alpha negative control (second triplicate) 2969.000 3563.444 1.000 -10395.17 16333.17
E. coli DH5 alpha negative control (third triplicate) 2574.667 3563.444 1.000 -10789.50 15938.83
E. coli DH5 alpha P3 (second triplicate) E. coli DH5 alpha (first triplicate) 5821.667 3563.444 .967 -7542.50 19185.83
E. coli DH5 alpha (second triplicate) 5489.667 3563.444 .981 -7874.50 18853.83
E. coli DH5 alpha (third triplicate) 5834.667 3563.444 .967 -7529.50 19198.83
E. coli DH5 alpha P1 (first triplicate) -69404.333* 3563.444 .000 -82768.50 -56040.17
E. coli DH5 alpha P1 (second triplicate) -61824.333* 3563.444 .000 -75188.50 -48460.17
E. coli DH5 alpha P1 (third triplicate) -60297.333* 3563.444 .000 -73661.50 -46933.17
E. coli DH5 alpha P2 (first triplicate) -24628.333* 3563.444 .000 -37992.50 -11264.17
E. coli DH5 alpha P2 (second triplicate) -35072.000* 3563.444 .000 -48436.17 -21707.83
E. coli DH5 alpha P2 (third triplicate) -29395.333* 3563.444 .000 -42759.50 -16031.17
E. coli DH5 alpha P3 (first triplicate) 1372.000 3563.444 1.000 -11992.17 14736.17
E. coli DH5 alpha P3 (third triplicate) 1377.333 3563.444 1.000 -11986.83 14741.50
E. coli DH5 alpha Positive control (first triplicate) -15817.667* 3563.444 .008 -29181.83 -2453.50
E. coli DH5 alpha Positive control (second triplicate) -16639.667* 3563.444 .004 -30003.83 -3275.50
E. coli DH5 alpha Positive control (third triplicate) -16713.333* 3563.444 .004 -30077.50 -3349.17
E. coli DH5 alpha negative control (first triplicate) 3741.667 3563.444 1.000 -9622.50 17105.83
E. coli DH5 alpha negative control (second triplicate) 4341.000 3563.444 .998 -9023.17 17705.17
E. coli DH5 alpha negative control (third triplicate) 3946.667 3563.444 .999 -9417.50 17310.83
E. coli DH5 alpha P3 (third triplicate) E. coli DH5 alpha (first triplicate) 4444.333 3563.444 .998 -8919.83 17808.50
E. coli DH5 alpha (second triplicate) 4112.333 3563.444 .999 -9251.83 17476.50
E. coli DH5 alpha (third triplicate) 4457.333 3563.444 .998 -8906.83 17821.50
E. coli DH5 alpha P1 (first triplicate) -70781.667* 3563.444 .000 -84145.83 -57417.50
E. coli DH5 alpha P1 (second triplicate) -63201.667* 3563.444 .000 -76565.83 -49837.50
E. coli DH5 alpha P1 (third triplicate) -61674.667* 3563.444 .000 -75038.83 -48310.50
E. coli DH5 alpha P2 (first triplicate) -26005.667* 3563.444 .000 -39369.83 -12641.50
E. coli DH5 alpha P2 (second triplicate) -36449.333* 3563.444 .000 -49813.50 -23085.17
E. coli DH5 alpha P2 (third triplicate) -30772.667* 3563.444 .000 -44136.83 -17408.50
E. coli DH5 alpha P3 (first triplicate) -5.333 3563.444 1.000 -13369.50 13358.83
E. coli DH5 alpha P3 (second triplicate) -1377.333 3563.444 1.000 -14741.50 11986.83
E. coli DH5 alpha Positive control (first triplicate) -17195.000* 3563.444 .003 -30559.17 -3830.83
E. coli DH5 alpha Positive control (second triplicate) -18017.000* 3563.444 .001 -31381.17 -4652.83
E. coli DH5 alpha Positive control (third triplicate) -18090.667* 3563.444 .001 -31454.83 -4726.50
E. coli DH5 alpha negative control (first triplicate) 2364.333 3563.444 1.000 -10999.83 15728.50
E. coli DH5 alpha negative control (second triplicate) 2963.667 3563.444 1.000 -10400.50 16327.83
E. coli DH5 alpha negative control (third triplicate) 2569.333 3563.444 1.000 -10794.83 15933.50
E. coli DH5 alpha Positive control (first triplicate) E. coli DH5 alpha (first triplicate) 21639.333* 3563.444 .000 8275.17 35003.50
E. coli DH5 alpha (second triplicate) 21307.333* 3563.444 .000 7943.17 34671.50
E. coli DH5 alpha (third triplicate) 21652.333* 3563.444 .000 8288.17 35016.50
E. coli DH5 alpha P1 (first triplicate) -53586.667* 3563.444 .000 -66950.83 -40222.50
E. coli DH5 alpha P1 (second triplicate) -46006.667* 3563.444 .000 -59370.83 -32642.50
E. coli DH5 alpha P1 (third triplicate) -44479.667* 3563.444 .000 -57843.83 -31115.50
E. coli DH5 alpha P2 (first triplicate) -8810.667 3563.444 .563 -22174.83 4553.50
E. coli DH5 alpha P2 (second triplicate) -19254.333* 3563.444 .001 -32618.50 -5890.17
E. coli DH5 alpha P2 (third triplicate) -13577.667* 3563.444 .043 -26941.83 -213.50
E. coli DH5 alpha P3 (first triplicate) 17189.667* 3563.444 .003 3825.50 30553.83
E. coli DH5 alpha P3 (second triplicate) 15817.667* 3563.444 .008 2453.50 29181.83
E. coli DH5 alpha P3 (third triplicate) 17195.000* 3563.444 .003 3830.83 30559.17
E. coli DH5 alpha Positive control (second triplicate) -822.000 3563.444 1.000 -14186.17 12542.17
E. coli DH5 alpha Positive control (third triplicate) -895.667 3563.444 1.000 -14259.83 12468.50
E. coli DH5 alpha negative control (first triplicate) 19559.333* 3563.444 .000 6195.17 32923.50
E. coli DH5 alpha negative control (second triplicate) 20158.667* 3563.444 .000 6794.50 33522.83
E. coli DH5 alpha negative control (third triplicate) 19764.333* 3563.444 .000 6400.17 33128.50
E. coli DH5 alpha Positive control (second triplicate) E. coli DH5 alpha (first triplicate) 22461.333* 3563.444 .000 9097.17 35825.50
E. coli DH5 alpha (second triplicate) 22129.333* 3563.444 .000 8765.17 35493.50
E. coli DH5 alpha (third triplicate) 22474.333* 3563.444 .000 9110.17 35838.50
E. coli DH5 alpha P1 (first triplicate) -52764.667* 3563.444 .000 -66128.83 -39400.50
E. coli DH5 alpha P1 (second triplicate) -45184.667* 3563.444 .000 -58548.83 -31820.50
E. coli DH5 alpha P1 (third triplicate) -43657.667* 3563.444 .000 -57021.83 -30293.50
E. coli DH5 alpha P2 (first triplicate) -7988.667 3563.444 .715 -21352.83 5375.50
E. coli DH5 alpha P2 (second triplicate) -18432.333* 3563.444 .001 -31796.50 -5068.17
E. coli DH5 alpha P2 (third triplicate) -12755.667 3563.444 .075 -26119.83 608.50
E. coli DH5 alpha P3 (first triplicate) 18011.667* 3563.444 .001 4647.50 31375.83
E. coli DH5 alpha P3 (second triplicate) 16639.667* 3563.444 .004 3275.50 30003.83
E. coli DH5 alpha P3 (third triplicate) 18017.000* 3563.444 .001 4652.83 31381.17
E. coli DH5 alpha Positive control (first triplicate) 822.000 3563.444 1.000 -12542.17 14186.17
E. coli DH5 alpha Positive control (third triplicate) -73.667 3563.444 1.000 -13437.83 13290.50
E. coli DH5 alpha negative control (first triplicate) 20381.333* 3563.444 .000 7017.17 33745.50
E. coli DH5 alpha negative control (second triplicate) 20980.667* 3563.444 .000 7616.50 34344.83
E. coli DH5 alpha negative control (third triplicate) 20586.333* 3563.444 .000 7222.17 33950.50
E. coli DH5 alpha Positive control (third triplicate) E. coli DH5 alpha (first triplicate) 22535.000* 3563.444 .000 9170.83 35899.17
E. coli DH5 alpha (second triplicate) 22203.000* 3563.444 .000 8838.83 35567.17
E. coli DH5 alpha (third triplicate) 22548.000* 3563.444 .000 9183.83 35912.17
E. coli DH5 alpha P1 (first triplicate) -52691.000* 3563.444 .000 -66055.17 -39326.83
E. coli DH5 alpha P1 (second triplicate) -45111.000* 3563.444 .000 -58475.17 -31746.83
E. coli DH5 alpha P1 (third triplicate) -43584.000* 3563.444 .000 -56948.17 -30219.83
E. coli DH5 alpha P2 (first triplicate) -7915.000 3563.444 .728 -21279.17 5449.17
E. coli DH5 alpha P2 (second triplicate) -18358.667* 3563.444 .001 -31722.83 -4994.50
E. coli DH5 alpha P2 (third triplicate) -12682.000 3563.444 .079 -26046.17 682.17
E. coli DH5 alpha P3 (first triplicate) 18085.333* 3563.444 .001 4721.17 31449.50
E. coli DH5 alpha P3 (second triplicate) 16713.333* 3563.444 .004 3349.17 30077.50
E. coli DH5 alpha P3 (third triplicate) 18090.667* 3563.444 .001 4726.50 31454.83
E. coli DH5 alpha Positive control (first triplicate) 895.667 3563.444 1.000 -12468.50 14259.83
E. coli DH5 alpha Positive control (second triplicate) 73.667 3563.444 1.000 -13290.50 13437.83
E. coli DH5 alpha negative control (first triplicate) 20455.000* 3563.444 .000 7090.83 33819.17
E. coli DH5 alpha negative control (second triplicate) 21054.333* 3563.444 .000 7690.17 34418.50
E. coli DH5 alpha negative control (third triplicate) 20660.000* 3563.444 .000 7295.83 34024.17
E. coli DH5 alpha negative control (first triplicate) E. coli DH5 alpha (first triplicate) 2080.000 3563.444 1.000 -11284.17 15444.17
E. coli DH5 alpha (second triplicate) 1748.000 3563.444 1.000 -11616.17 15112.17
E. coli DH5 alpha (third triplicate) 2093.000 3563.444 1.000 -11271.17 15457.17
E. coli DH5 alpha P1 (first triplicate) -73146.000* 3563.444 .000 -86510.17 -59781.83
E. coli DH5 alpha P1 (second triplicate) -65566.000* 3563.444 .000 -78930.17 -52201.83
E. coli DH5 alpha P1 (third triplicate) -64039.000* 3563.444 .000 -77403.17 -50674.83
E. coli DH5 alpha P2 (first triplicate) -28370.000* 3563.444 .000 -41734.17 -15005.83
E. coli DH5 alpha P2 (second triplicate) -38813.667* 3563.444 .000 -52177.83 -25449.50
E. coli DH5 alpha P2 (third triplicate) -33137.000* 3563.444 .000 -46501.17 -19772.83
E. coli DH5 alpha P3 (first triplicate) -2369.667 3563.444 1.000 -15733.83 10994.50
E. coli DH5 alpha P3 (second triplicate) -3741.667 3563.444 1.000 -17105.83 9622.50
E. coli DH5 alpha P3 (third triplicate) -2364.333 3563.444 1.000 -15728.50 10999.83
E. coli DH5 alpha Positive control (first triplicate) -19559.333* 3563.444 .000 -32923.50 -6195.17
E. coli DH5 alpha Positive control (second triplicate) -20381.333* 3563.444 .000 -33745.50 -7017.17
E. coli DH5 alpha Positive control (third triplicate) -20455.000* 3563.444 .000 -33819.17 -7090.83
E. coli DH5 alpha negative control (second triplicate) 599.333 3563.444 1.000 -12764.83 13963.50
E. coli DH5 alpha negative control (third triplicate) 205.000 3563.444 1.000 -13159.17 13569.17
E. coli DH5 alpha negative control (second triplicate) E. coli DH5 alpha (first triplicate) 1480.667 3563.444 1.000 -11883.50 14844.83
E. coli DH5 alpha (second triplicate) 1148.667 3563.444 1.000 -12215.50 14512.83
E. coli DH5 alpha (third triplicate) 1493.667 3563.444 1.000 -11870.50 14857.83
E. coli DH5 alpha P1 (first triplicate) -73745.333* 3563.444 .000 -87109.50 -60381.17
E. coli DH5 alpha P1 (second triplicate) -66165.333* 3563.444 .000 -79529.50 -52801.17
E. coli DH5 alpha P1 (third triplicate) -64638.333* 3563.444 .000 -78002.50 -51274.17
E. coli DH5 alpha P2 (first triplicate) -28969.333* 3563.444 .000 -42333.50 -15605.17
E. coli DH5 alpha P2 (second triplicate) -39413.000* 3563.444 .000 -52777.17 -26048.83
E. coli DH5 alpha P2 (third triplicate) -33736.333* 3563.444 .000 -47100.50 -20372.17
E. coli DH5 alpha P3 (first triplicate) -2969.000 3563.444 1.000 -16333.17 10395.17
E. coli DH5 alpha P3 (second triplicate) -4341.000 3563.444 .998 -17705.17 9023.17
E. coli DH5 alpha P3 (third triplicate) -2963.667 3563.444 1.000 -16327.83 10400.50
E. coli DH5 alpha Positive control (first triplicate) -20158.667* 3563.444 .000 -33522.83 -6794.50
E. coli DH5 alpha Positive control (second triplicate) -20980.667* 3563.444 .000 -34344.83 -7616.50
E. coli DH5 alpha Positive control (third triplicate) -21054.333* 3563.444 .000 -34418.50 -7690.17
E. coli DH5 alpha negative control (first triplicate) -599.333 3563.444 1.000 -13963.50 12764.83
E. coli DH5 alpha negative control (third triplicate) -394.333 3563.444 1.000 -13758.50 12969.83
E. coli DH5 alpha negative control (third triplicate) E. coli DH5 alpha (first triplicate) 1875.000 3563.444 1.000 -11489.17 15239.17
E. coli DH5 alpha (second triplicate) 1543.000 3563.444 1.000 -11821.17 14907.17
E. coli DH5 alpha (third triplicate) 1888.000 3563.444 1.000 -11476.17 15252.17
E. coli DH5 alpha P1 (first triplicate) -73351.000* 3563.444 .000 -86715.17 -59986.83
E. coli DH5 alpha P1 (second triplicate) -65771.000* 3563.444 .000 -79135.17 -52406.83
E. coli DH5 alpha P1 (third triplicate) -64244.000* 3563.444 .000 -77608.17 -50879.83
E. coli DH5 alpha P2 (first triplicate) -28575.000* 3563.444 .000 -41939.17 -15210.83
E. coli DH5 alpha P2 (second triplicate) -39018.667* 3563.444 .000 -52382.83 -25654.50
E. coli DH5 alpha P2 (third triplicate) -33342.000* 3563.444 .000 -46706.17 -19977.83
E. coli DH5 alpha P3 (first triplicate) -2574.667 3563.444 1.000 -15938.83 10789.50
E. coli DH5 alpha P3 (second triplicate) -3946.667 3563.444 .999 -17310.83 9417.50
E. coli DH5 alpha P3 (third triplicate) -2569.333 3563.444 1.000 -15933.50 10794.83
E. coli DH5 alpha Positive control (first triplicate) -19764.333* 3563.444 .000 -33128.50 -6400.17
E. coli DH5 alpha Positive control (second triplicate) -20586.333* 3563.444 .000 -33950.50 -7222.17
E. coli DH5 alpha Positive control (third triplicate) -20660.000* 3563.444 .000 -34024.17 -7295.83
E. coli DH5 alpha negative control (first triplicate) -205.000 3563.444 1.000 -13569.17 13159.17
E. coli DH5 alpha negative control (second triplicate) 394.333 3563.444 1.000 -12969.83 13758.50
*. The mean difference is significant at the 0.05 level.