Team:Birkbeck/InterLab Study/fluorescence ANOVA ungrouped

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Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




ANOVA of Fluorescence between strains of E. coli across groups.htm

ANOVA Table of E. coli DH5α Strains Fluorescence.
Fluorescence
  Sum of Squares df Mean Square F Sig.
Between Groups 33009519007.426 17 1941736412.202 101.943 .000
Within Groups 685699234.000 36 19047200.944    
Total 33695218241.426 53      

Multiple Comparisons of Fluorescence of E. coli DH5α Strains.
Fluorescence
Tukey HSD
(I) GroupingTtest (J) GroupingTtest Mean Difference (I-J) Std. Error Sig. 95% Confidence Interval
Lower Bound Upper Bound
E. coli DH5 alpha (first triplicate) E. coli DH5 alpha (second triplicate) -332.000 3563.444 1.000 -13696.17 13032.17
E. coli DH5 alpha (third triplicate) 13.000 3563.444 1.000 -13351.17 13377.17
E. coli DH5 alpha P1 (first triplicate) -75226.000* 3563.444 .000 -88590.17 -61861.83
E. coli DH5 alpha P1 (second triplicate) -67646.000* 3563.444 .000 -81010.17 -54281.83
E. coli DH5 alpha P1 (third triplicate) -66119.000* 3563.444 .000 -79483.17 -52754.83
E. coli DH5 alpha P2 (first triplicate) -30450.000* 3563.444 .000 -43814.17 -17085.83
E. coli DH5 alpha P2 (second triplicate) -40893.667* 3563.444 .000 -54257.83 -27529.50
E. coli DH5 alpha P2 (third triplicate) -35217.000* 3563.444 .000 -48581.17 -21852.83
E. coli DH5 alpha P3 (first triplicate) -4449.667 3563.444 .998 -17813.83 8914.50
E. coli DH5 alpha P3 (second triplicate) -5821.667 3563.444 .967 -19185.83 7542.50
E. coli DH5 alpha P3 (third triplicate) -4444.333 3563.444 .998 -17808.50 8919.83
E. coli DH5 alpha Positive control (first triplicate) -21639.333* 3563.444 .000 -35003.50 -8275.17
E. coli DH5 alpha Positive control (second triplicate) -22461.333* 3563.444 .000 -35825.50 -9097.17
E. coli DH5 alpha Positive control (third triplicate) -22535.000* 3563.444 .000 -35899.17 -9170.83
E. coli DH5 alpha negative control (first triplicate) -2080.000 3563.444 1.000 -15444.17 11284.17
E. coli DH5 alpha negative control (second triplicate) -1480.667 3563.444 1.000 -14844.83 11883.50
E. coli DH5 alpha negative control (third triplicate) -1875.000 3563.444 1.000 -15239.17 11489.17
E. coli DH5 alpha (second triplicate) E. coli DH5 alpha (first triplicate) 332.000 3563.444 1.000 -13032.17 13696.17
E. coli DH5 alpha (third triplicate) 345.000 3563.444 1.000 -13019.17 13709.17
E. coli DH5 alpha P1 (first triplicate) -74894.000* 3563.444 .000 -88258.17 -61529.83
E. coli DH5 alpha P1 (second triplicate) -67314.000* 3563.444 .000 -80678.17 -53949.83
E. coli DH5 alpha P1 (third triplicate) -65787.000* 3563.444 .000 -79151.17 -52422.83
E. coli DH5 alpha P2 (first triplicate) -30118.000* 3563.444 .000 -43482.17 -16753.83
E. coli DH5 alpha P2 (second triplicate) -40561.667* 3563.444 .000 -53925.83 -27197.50
E. coli DH5 alpha P2 (third triplicate) -34885.000* 3563.444 .000 -48249.17 -21520.83
E. coli DH5 alpha P3 (first triplicate) -4117.667 3563.444 .999 -17481.83 9246.50
E. coli DH5 alpha P3 (second triplicate) -5489.667 3563.444 .981 -18853.83 7874.50
E. coli DH5 alpha P3 (third triplicate) -4112.333 3563.444 .999 -17476.50 9251.83
E. coli DH5 alpha Positive control (first triplicate) -21307.333* 3563.444 .000 -34671.50 -7943.17
E. coli DH5 alpha Positive control (second triplicate) -22129.333* 3563.444 .000 -35493.50 -8765.17
E. coli DH5 alpha Positive control (third triplicate) -22203.000* 3563.444 .000 -35567.17 -8838.83
E. coli DH5 alpha negative control (first triplicate) -1748.000 3563.444 1.000 -15112.17 11616.17
E. coli DH5 alpha negative control (second triplicate) -1148.667 3563.444 1.000 -14512.83 12215.50
E. coli DH5 alpha negative control (third triplicate) -1543.000 3563.444 1.000 -14907.17 11821.17
E. coli DH5 alpha (third triplicate) E. coli DH5 alpha (first triplicate) -13.000 3563.444 1.000 -13377.17 13351.17
E. coli DH5 alpha (second triplicate) -345.000 3563.444 1.000 -13709.17 13019.17
E. coli DH5 alpha P1 (first triplicate) -75239.000* 3563.444 .000 -88603.17 -61874.83
E. coli DH5 alpha P1 (second triplicate) -67659.000* 3563.444 .000 -81023.17 -54294.83
E. coli DH5 alpha P1 (third triplicate) -66132.000* 3563.444 .000 -79496.17 -52767.83
E. coli DH5 alpha P2 (first triplicate) -30463.000* 3563.444 .000 -43827.17 -17098.83
E. coli DH5 alpha P2 (second triplicate) -40906.667* 3563.444 .000 -54270.83 -27542.50
E. coli DH5 alpha P2 (third triplicate) -35230.000* 3563.444 .000 -48594.17 -21865.83
E. coli DH5 alpha P3 (first triplicate) -4462.667 3563.444 .998 -17826.83 8901.50
E. coli DH5 alpha P3 (second triplicate) -5834.667 3563.444 .967 -19198.83 7529.50
E. coli DH5 alpha P3 (third triplicate) -4457.333 3563.444 .998 -17821.50 8906.83
E. coli DH5 alpha Positive control (first triplicate) -21652.333* 3563.444 .000 -35016.50 -8288.17
E. coli DH5 alpha Positive control (second triplicate) -22474.333* 3563.444 .000 -35838.50 -9110.17
E. coli DH5 alpha Positive control (third triplicate) -22548.000* 3563.444 .000 -35912.17 -9183.83
E. coli DH5 alpha negative control (first triplicate) -2093.000 3563.444 1.000 -15457.17 11271.17
E. coli DH5 alpha negative control (second triplicate) -1493.667 3563.444 1.000 -14857.83 11870.50
E. coli DH5 alpha negative control (third triplicate) -1888.000 3563.444 1.000 -15252.17 11476.17
E. coli DH5 alpha P1 (first triplicate) E. coli DH5 alpha (first triplicate) 75226.000* 3563.444 .000 61861.83 88590.17
E. coli DH5 alpha (second triplicate) 74894.000* 3563.444 .000 61529.83 88258.17
E. coli DH5 alpha (third triplicate) 75239.000* 3563.444 .000 61874.83 88603.17
E. coli DH5 alpha P1 (second triplicate) 7580.000 3563.444 .784 -5784.17 20944.17
E. coli DH5 alpha P1 (third triplicate) 9107.000 3563.444 .508 -4257.17 22471.17
E. coli DH5 alpha P2 (first triplicate) 44776.000* 3563.444 .000 31411.83 58140.17
E. coli DH5 alpha P2 (second triplicate) 34332.333* 3563.444 .000 20968.17 47696.50
E. coli DH5 alpha P2 (third triplicate) 40009.000* 3563.444 .000 26644.83 53373.17
E. coli DH5 alpha P3 (first triplicate) 70776.333* 3563.444 .000 57412.17 84140.50
E. coli DH5 alpha P3 (second triplicate) 69404.333* 3563.444 .000 56040.17 82768.50
E. coli DH5 alpha P3 (third triplicate) 70781.667* 3563.444 .000 57417.50 84145.83
E. coli DH5 alpha Positive control (first triplicate) 53586.667* 3563.444 .000 40222.50 66950.83
E. coli DH5 alpha Positive control (second triplicate) 52764.667* 3563.444 .000 39400.50 66128.83
E. coli DH5 alpha Positive control (third triplicate) 52691.000* 3563.444 .000 39326.83 66055.17
E. coli DH5 alpha negative control (first triplicate) 73146.000* 3563.444 .000 59781.83 86510.17
E. coli DH5 alpha negative control (second triplicate) 73745.333* 3563.444 .000 60381.17 87109.50
E. coli DH5 alpha negative control (third triplicate) 73351.000* 3563.444 .000 59986.83 86715.17
E. coli DH5 alpha P1 (second triplicate) E. coli DH5 alpha (first triplicate) 67646.000* 3563.444 .000 54281.83 81010.17
E. coli DH5 alpha (second triplicate) 67314.000* 3563.444 .000 53949.83 80678.17
E. coli DH5 alpha (third triplicate) 67659.000* 3563.444 .000 54294.83 81023.17
E. coli DH5 alpha P1 (first triplicate) -7580.000 3563.444 .784 -20944.17 5784.17
E. coli DH5 alpha P1 (third triplicate) 1527.000 3563.444 1.000 -11837.17 14891.17
E. coli DH5 alpha P2 (first triplicate) 37196.000* 3563.444 .000 23831.83 50560.17
E. coli DH5 alpha P2 (second triplicate) 26752.333* 3563.444 .000 13388.17 40116.50
E. coli DH5 alpha P2 (third triplicate) 32429.000* 3563.444 .000 19064.83 45793.17
E. coli DH5 alpha P3 (first triplicate) 63196.333* 3563.444 .000 49832.17 76560.50
E. coli DH5 alpha P3 (second triplicate) 61824.333* 3563.444 .000 48460.17 75188.50
E. coli DH5 alpha P3 (third triplicate) 63201.667* 3563.444 .000 49837.50 76565.83
E. coli DH5 alpha Positive control (first triplicate) 46006.667* 3563.444 .000 32642.50 59370.83
E. coli DH5 alpha Positive control (second triplicate) 45184.667* 3563.444 .000 31820.50 58548.83
E. coli DH5 alpha Positive control (third triplicate) 45111.000* 3563.444 .000 31746.83 58475.17
E. coli DH5 alpha negative control (first triplicate) 65566.000* 3563.444 .000 52201.83 78930.17
E. coli DH5 alpha negative control (second triplicate) 66165.333* 3563.444 .000 52801.17 79529.50
E. coli DH5 alpha negative control (third triplicate) 65771.000* 3563.444 .000 52406.83 79135.17
E. coli DH5 alpha P1 (third triplicate) E. coli DH5 alpha (first triplicate) 66119.000* 3563.444 .000 52754.83 79483.17
E. coli DH5 alpha (second triplicate) 65787.000* 3563.444 .000 52422.83 79151.17
E. coli DH5 alpha (third triplicate) 66132.000* 3563.444 .000 52767.83 79496.17
E. coli DH5 alpha P1 (first triplicate) -9107.000 3563.444 .508 -22471.17 4257.17
E. coli DH5 alpha P1 (second triplicate) -1527.000 3563.444 1.000 -14891.17 11837.17
E. coli DH5 alpha P2 (first triplicate) 35669.000* 3563.444 .000 22304.83 49033.17
E. coli DH5 alpha P2 (second triplicate) 25225.333* 3563.444 .000 11861.17 38589.50
E. coli DH5 alpha P2 (third triplicate) 30902.000* 3563.444 .000 17537.83 44266.17
E. coli DH5 alpha P3 (first triplicate) 61669.333* 3563.444 .000 48305.17 75033.50
E. coli DH5 alpha P3 (second triplicate) 60297.333* 3563.444 .000 46933.17 73661.50
E. coli DH5 alpha P3 (third triplicate) 61674.667* 3563.444 .000 48310.50 75038.83
E. coli DH5 alpha Positive control (first triplicate) 44479.667* 3563.444 .000 31115.50 57843.83
E. coli DH5 alpha Positive control (second triplicate) 43657.667* 3563.444 .000 30293.50 57021.83
E. coli DH5 alpha Positive control (third triplicate) 43584.000* 3563.444 .000 30219.83 56948.17
E. coli DH5 alpha negative control (first triplicate) 64039.000* 3563.444 .000 50674.83 77403.17
E. coli DH5 alpha negative control (second triplicate) 64638.333* 3563.444 .000 51274.17 78002.50
E. coli DH5 alpha negative control (third triplicate) 64244.000* 3563.444 .000 50879.83 77608.17
E. coli DH5 alpha P2 (first triplicate) E. coli DH5 alpha (first triplicate) 30450.000* 3563.444 .000 17085.83 43814.17
E. coli DH5 alpha (second triplicate) 30118.000* 3563.444 .000 16753.83 43482.17
E. coli DH5 alpha (third triplicate) 30463.000* 3563.444 .000 17098.83 43827.17
E. coli DH5 alpha P1 (first triplicate) -44776.000* 3563.444 .000 -58140.17 -31411.83
E. coli DH5 alpha P1 (second triplicate) -37196.000* 3563.444 .000 -50560.17 -23831.83
E. coli DH5 alpha P1 (third triplicate) -35669.000* 3563.444 .000 -49033.17 -22304.83
E. coli DH5 alpha P2 (second triplicate) -10443.667 3563.444 .285 -23807.83 2920.50
E. coli DH5 alpha P2 (third triplicate) -4767.000 3563.444 .995 -18131.17 8597.17
E. coli DH5 alpha P3 (first triplicate) 26000.333* 3563.444 .000 12636.17 39364.50
E. coli DH5 alpha P3 (second triplicate) 24628.333* 3563.444 .000 11264.17 37992.50
E. coli DH5 alpha P3 (third triplicate) 26005.667* 3563.444 .000 12641.50 39369.83
E. coli DH5 alpha Positive control (first triplicate) 8810.667 3563.444 .563 -4553.50 22174.83
E. coli DH5 alpha Positive control (second triplicate) 7988.667 3563.444 .715 -5375.50 21352.83
E. coli DH5 alpha Positive control (third triplicate) 7915.000 3563.444 .728 -5449.17 21279.17
E. coli DH5 alpha negative control (first triplicate) 28370.000* 3563.444 .000 15005.83 41734.17
E. coli DH5 alpha negative control (second triplicate) 28969.333* 3563.444 .000 15605.17 42333.50
E. coli DH5 alpha negative control (third triplicate) 28575.000* 3563.444 .000 15210.83 41939.17
E. coli DH5 alpha P2 (second triplicate) E. coli DH5 alpha (first triplicate) 40893.667* 3563.444 .000 27529.50 54257.83
E. coli DH5 alpha (second triplicate) 40561.667* 3563.444 .000 27197.50 53925.83
E. coli DH5 alpha (third triplicate) 40906.667* 3563.444 .000 27542.50 54270.83
E. coli DH5 alpha P1 (first triplicate) -34332.333* 3563.444 .000 -47696.50 -20968.17
E. coli DH5 alpha P1 (second triplicate) -26752.333* 3563.444 .000 -40116.50 -13388.17
E. coli DH5 alpha P1 (third triplicate) -25225.333* 3563.444 .000 -38589.50 -11861.17
E. coli DH5 alpha P2 (first triplicate) 10443.667 3563.444 .285 -2920.50 23807.83
E. coli DH5 alpha P2 (third triplicate) 5676.667 3563.444 .974 -7687.50 19040.83
E. coli DH5 alpha P3 (first triplicate) 36444.000* 3563.444 .000 23079.83 49808.17
E. coli DH5 alpha P3 (second triplicate) 35072.000* 3563.444 .000 21707.83 48436.17
E. coli DH5 alpha P3 (third triplicate) 36449.333* 3563.444 .000 23085.17 49813.50
E. coli DH5 alpha Positive control (first triplicate) 19254.333* 3563.444 .001 5890.17 32618.50
E. coli DH5 alpha Positive control (second triplicate) 18432.333* 3563.444 .001 5068.17 31796.50
E. coli DH5 alpha Positive control (third triplicate) 18358.667* 3563.444 .001 4994.50 31722.83
E. coli DH5 alpha negative control (first triplicate) 38813.667* 3563.444 .000 25449.50 52177.83
E. coli DH5 alpha negative control (second triplicate) 39413.000* 3563.444 .000 26048.83 52777.17
E. coli DH5 alpha negative control (third triplicate) 39018.667* 3563.444 .000 25654.50 52382.83
E. coli DH5 alpha P2 (third triplicate) E. coli DH5 alpha (first triplicate) 35217.000* 3563.444 .000 21852.83 48581.17
E. coli DH5 alpha (second triplicate) 34885.000* 3563.444 .000 21520.83 48249.17
E. coli DH5 alpha (third triplicate) 35230.000* 3563.444 .000 21865.83 48594.17
E. coli DH5 alpha P1 (first triplicate) -40009.000* 3563.444 .000 -53373.17 -26644.83
E. coli DH5 alpha P1 (second triplicate) -32429.000* 3563.444 .000 -45793.17 -19064.83
E. coli DH5 alpha P1 (third triplicate) -30902.000* 3563.444 .000 -44266.17 -17537.83
E. coli DH5 alpha P2 (first triplicate) 4767.000 3563.444 .995 -8597.17 18131.17
E. coli DH5 alpha P2 (second triplicate) -5676.667 3563.444 .974 -19040.83 7687.50
E. coli DH5 alpha P3 (first triplicate) 30767.333* 3563.444 .000 17403.17 44131.50
E. coli DH5 alpha P3 (second triplicate) 29395.333* 3563.444 .000 16031.17 42759.50
E. coli DH5 alpha P3 (third triplicate) 30772.667* 3563.444 .000 17408.50 44136.83
E. coli DH5 alpha Positive control (first triplicate) 13577.667* 3563.444 .043 213.50 26941.83
E. coli DH5 alpha Positive control (second triplicate) 12755.667 3563.444 .075 -608.50 26119.83
E. coli DH5 alpha Positive control (third triplicate) 12682.000 3563.444 .079 -682.17 26046.17
E. coli DH5 alpha negative control (first triplicate) 33137.000* 3563.444 .000 19772.83 46501.17
E. coli DH5 alpha negative control (second triplicate) 33736.333* 3563.444 .000 20372.17 47100.50
E. coli DH5 alpha negative control (third triplicate) 33342.000* 3563.444 .000 19977.83 46706.17
E. coli DH5 alpha P3 (first triplicate) E. coli DH5 alpha (first triplicate) 4449.667 3563.444 .998 -8914.50 17813.83
E. coli DH5 alpha (second triplicate) 4117.667 3563.444 .999 -9246.50 17481.83
E. coli DH5 alpha (third triplicate) 4462.667 3563.444 .998 -8901.50 17826.83
E. coli DH5 alpha P1 (first triplicate) -70776.333* 3563.444 .000 -84140.50 -57412.17
E. coli DH5 alpha P1 (second triplicate) -63196.333* 3563.444 .000 -76560.50 -49832.17
E. coli DH5 alpha P1 (third triplicate) -61669.333* 3563.444 .000 -75033.50 -48305.17
E. coli DH5 alpha P2 (first triplicate) -26000.333* 3563.444 .000 -39364.50 -12636.17
E. coli DH5 alpha P2 (second triplicate) -36444.000* 3563.444 .000 -49808.17 -23079.83
E. coli DH5 alpha P2 (third triplicate) -30767.333* 3563.444 .000 -44131.50 -17403.17
E. coli DH5 alpha P3 (second triplicate) -1372.000 3563.444 1.000 -14736.17 11992.17
E. coli DH5 alpha P3 (third triplicate) 5.333 3563.444 1.000 -13358.83 13369.50
E. coli DH5 alpha Positive control (first triplicate) -17189.667* 3563.444 .003 -30553.83 -3825.50
E. coli DH5 alpha Positive control (second triplicate) -18011.667* 3563.444 .001 -31375.83 -4647.50
E. coli DH5 alpha Positive control (third triplicate) -18085.333* 3563.444 .001 -31449.50 -4721.17
E. coli DH5 alpha negative control (first triplicate) 2369.667 3563.444 1.000 -10994.50 15733.83
E. coli DH5 alpha negative control (second triplicate) 2969.000 3563.444 1.000 -10395.17 16333.17
E. coli DH5 alpha negative control (third triplicate) 2574.667 3563.444 1.000 -10789.50 15938.83
E. coli DH5 alpha P3 (second triplicate) E. coli DH5 alpha (first triplicate) 5821.667 3563.444 .967 -7542.50 19185.83
E. coli DH5 alpha (second triplicate) 5489.667 3563.444 .981 -7874.50 18853.83
E. coli DH5 alpha (third triplicate) 5834.667 3563.444 .967 -7529.50 19198.83
E. coli DH5 alpha P1 (first triplicate) -69404.333* 3563.444 .000 -82768.50 -56040.17
E. coli DH5 alpha P1 (second triplicate) -61824.333* 3563.444 .000 -75188.50 -48460.17
E. coli DH5 alpha P1 (third triplicate) -60297.333* 3563.444 .000 -73661.50 -46933.17
E. coli DH5 alpha P2 (first triplicate) -24628.333* 3563.444 .000 -37992.50 -11264.17
E. coli DH5 alpha P2 (second triplicate) -35072.000* 3563.444 .000 -48436.17 -21707.83
E. coli DH5 alpha P2 (third triplicate) -29395.333* 3563.444 .000 -42759.50 -16031.17
E. coli DH5 alpha P3 (first triplicate) 1372.000 3563.444 1.000 -11992.17 14736.17
E. coli DH5 alpha P3 (third triplicate) 1377.333 3563.444 1.000 -11986.83 14741.50
E. coli DH5 alpha Positive control (first triplicate) -15817.667* 3563.444 .008 -29181.83 -2453.50
E. coli DH5 alpha Positive control (second triplicate) -16639.667* 3563.444 .004 -30003.83 -3275.50
E. coli DH5 alpha Positive control (third triplicate) -16713.333* 3563.444 .004 -30077.50 -3349.17
E. coli DH5 alpha negative control (first triplicate) 3741.667 3563.444 1.000 -9622.50 17105.83
E. coli DH5 alpha negative control (second triplicate) 4341.000 3563.444 .998 -9023.17 17705.17
E. coli DH5 alpha negative control (third triplicate) 3946.667 3563.444 .999 -9417.50 17310.83
E. coli DH5 alpha P3 (third triplicate) E. coli DH5 alpha (first triplicate) 4444.333 3563.444 .998 -8919.83 17808.50
E. coli DH5 alpha (second triplicate) 4112.333 3563.444 .999 -9251.83 17476.50
E. coli DH5 alpha (third triplicate) 4457.333 3563.444 .998 -8906.83 17821.50
E. coli DH5 alpha P1 (first triplicate) -70781.667* 3563.444 .000 -84145.83 -57417.50
E. coli DH5 alpha P1 (second triplicate) -63201.667* 3563.444 .000 -76565.83 -49837.50
E. coli DH5 alpha P1 (third triplicate) -61674.667* 3563.444 .000 -75038.83 -48310.50
E. coli DH5 alpha P2 (first triplicate) -26005.667* 3563.444 .000 -39369.83 -12641.50
E. coli DH5 alpha P2 (second triplicate) -36449.333* 3563.444 .000 -49813.50 -23085.17
E. coli DH5 alpha P2 (third triplicate) -30772.667* 3563.444 .000 -44136.83 -17408.50
E. coli DH5 alpha P3 (first triplicate) -5.333 3563.444 1.000 -13369.50 13358.83
E. coli DH5 alpha P3 (second triplicate) -1377.333 3563.444 1.000 -14741.50 11986.83
E. coli DH5 alpha Positive control (first triplicate) -17195.000* 3563.444 .003 -30559.17 -3830.83
E. coli DH5 alpha Positive control (second triplicate) -18017.000* 3563.444 .001 -31381.17 -4652.83
E. coli DH5 alpha Positive control (third triplicate) -18090.667* 3563.444 .001 -31454.83 -4726.50
E. coli DH5 alpha negative control (first triplicate) 2364.333 3563.444 1.000 -10999.83 15728.50
E. coli DH5 alpha negative control (second triplicate) 2963.667 3563.444 1.000 -10400.50 16327.83
E. coli DH5 alpha negative control (third triplicate) 2569.333 3563.444 1.000 -10794.83 15933.50
E. coli DH5 alpha Positive control (first triplicate) E. coli DH5 alpha (first triplicate) 21639.333* 3563.444 .000 8275.17 35003.50
E. coli DH5 alpha (second triplicate) 21307.333* 3563.444 .000 7943.17 34671.50
E. coli DH5 alpha (third triplicate) 21652.333* 3563.444 .000 8288.17 35016.50
E. coli DH5 alpha P1 (first triplicate) -53586.667* 3563.444 .000 -66950.83 -40222.50
E. coli DH5 alpha P1 (second triplicate) -46006.667* 3563.444 .000 -59370.83 -32642.50
E. coli DH5 alpha P1 (third triplicate) -44479.667* 3563.444 .000 -57843.83 -31115.50
E. coli DH5 alpha P2 (first triplicate) -8810.667 3563.444 .563 -22174.83 4553.50
E. coli DH5 alpha P2 (second triplicate) -19254.333* 3563.444 .001 -32618.50 -5890.17
E. coli DH5 alpha P2 (third triplicate) -13577.667* 3563.444 .043 -26941.83 -213.50
E. coli DH5 alpha P3 (first triplicate) 17189.667* 3563.444 .003 3825.50 30553.83
E. coli DH5 alpha P3 (second triplicate) 15817.667* 3563.444 .008 2453.50 29181.83
E. coli DH5 alpha P3 (third triplicate) 17195.000* 3563.444 .003 3830.83 30559.17
E. coli DH5 alpha Positive control (second triplicate) -822.000 3563.444 1.000 -14186.17 12542.17
E. coli DH5 alpha Positive control (third triplicate) -895.667 3563.444 1.000 -14259.83 12468.50
E. coli DH5 alpha negative control (first triplicate) 19559.333* 3563.444 .000 6195.17 32923.50
E. coli DH5 alpha negative control (second triplicate) 20158.667* 3563.444 .000 6794.50 33522.83
E. coli DH5 alpha negative control (third triplicate) 19764.333* 3563.444 .000 6400.17 33128.50
E. coli DH5 alpha Positive control (second triplicate) E. coli DH5 alpha (first triplicate) 22461.333* 3563.444 .000 9097.17 35825.50
E. coli DH5 alpha (second triplicate) 22129.333* 3563.444 .000 8765.17 35493.50
E. coli DH5 alpha (third triplicate) 22474.333* 3563.444 .000 9110.17 35838.50
E. coli DH5 alpha P1 (first triplicate) -52764.667* 3563.444 .000 -66128.83 -39400.50
E. coli DH5 alpha P1 (second triplicate) -45184.667* 3563.444 .000 -58548.83 -31820.50
E. coli DH5 alpha P1 (third triplicate) -43657.667* 3563.444 .000 -57021.83 -30293.50
E. coli DH5 alpha P2 (first triplicate) -7988.667 3563.444 .715 -21352.83 5375.50
E. coli DH5 alpha P2 (second triplicate) -18432.333* 3563.444 .001 -31796.50 -5068.17
E. coli DH5 alpha P2 (third triplicate) -12755.667 3563.444 .075 -26119.83 608.50
E. coli DH5 alpha P3 (first triplicate) 18011.667* 3563.444 .001 4647.50 31375.83
E. coli DH5 alpha P3 (second triplicate) 16639.667* 3563.444 .004 3275.50 30003.83
E. coli DH5 alpha P3 (third triplicate) 18017.000* 3563.444 .001 4652.83 31381.17
E. coli DH5 alpha Positive control (first triplicate) 822.000 3563.444 1.000 -12542.17 14186.17
E. coli DH5 alpha Positive control (third triplicate) -73.667 3563.444 1.000 -13437.83 13290.50
E. coli DH5 alpha negative control (first triplicate) 20381.333* 3563.444 .000 7017.17 33745.50
E. coli DH5 alpha negative control (second triplicate) 20980.667* 3563.444 .000 7616.50 34344.83
E. coli DH5 alpha negative control (third triplicate) 20586.333* 3563.444 .000 7222.17 33950.50
E. coli DH5 alpha Positive control (third triplicate) E. coli DH5 alpha (first triplicate) 22535.000* 3563.444 .000 9170.83 35899.17
E. coli DH5 alpha (second triplicate) 22203.000* 3563.444 .000 8838.83 35567.17
E. coli DH5 alpha (third triplicate) 22548.000* 3563.444 .000 9183.83 35912.17
E. coli DH5 alpha P1 (first triplicate) -52691.000* 3563.444 .000 -66055.17 -39326.83
E. coli DH5 alpha P1 (second triplicate) -45111.000* 3563.444 .000 -58475.17 -31746.83
E. coli DH5 alpha P1 (third triplicate) -43584.000* 3563.444 .000 -56948.17 -30219.83
E. coli DH5 alpha P2 (first triplicate) -7915.000 3563.444 .728 -21279.17 5449.17
E. coli DH5 alpha P2 (second triplicate) -18358.667* 3563.444 .001 -31722.83 -4994.50
E. coli DH5 alpha P2 (third triplicate) -12682.000 3563.444 .079 -26046.17 682.17
E. coli DH5 alpha P3 (first triplicate) 18085.333* 3563.444 .001 4721.17 31449.50
E. coli DH5 alpha P3 (second triplicate) 16713.333* 3563.444 .004 3349.17 30077.50
E. coli DH5 alpha P3 (third triplicate) 18090.667* 3563.444 .001 4726.50 31454.83
E. coli DH5 alpha Positive control (first triplicate) 895.667 3563.444 1.000 -12468.50 14259.83
E. coli DH5 alpha Positive control (second triplicate) 73.667 3563.444 1.000 -13290.50 13437.83
E. coli DH5 alpha negative control (first triplicate) 20455.000* 3563.444 .000 7090.83 33819.17
E. coli DH5 alpha negative control (second triplicate) 21054.333* 3563.444 .000 7690.17 34418.50
E. coli DH5 alpha negative control (third triplicate) 20660.000* 3563.444 .000 7295.83 34024.17
E. coli DH5 alpha negative control (first triplicate) E. coli DH5 alpha (first triplicate) 2080.000 3563.444 1.000 -11284.17 15444.17
E. coli DH5 alpha (second triplicate) 1748.000 3563.444 1.000 -11616.17 15112.17
E. coli DH5 alpha (third triplicate) 2093.000 3563.444 1.000 -11271.17 15457.17
E. coli DH5 alpha P1 (first triplicate) -73146.000* 3563.444 .000 -86510.17 -59781.83
E. coli DH5 alpha P1 (second triplicate) -65566.000* 3563.444 .000 -78930.17 -52201.83
E. coli DH5 alpha P1 (third triplicate) -64039.000* 3563.444 .000 -77403.17 -50674.83
E. coli DH5 alpha P2 (first triplicate) -28370.000* 3563.444 .000 -41734.17 -15005.83
E. coli DH5 alpha P2 (second triplicate) -38813.667* 3563.444 .000 -52177.83 -25449.50
E. coli DH5 alpha P2 (third triplicate) -33137.000* 3563.444 .000 -46501.17 -19772.83
E. coli DH5 alpha P3 (first triplicate) -2369.667 3563.444 1.000 -15733.83 10994.50
E. coli DH5 alpha P3 (second triplicate) -3741.667 3563.444 1.000 -17105.83 9622.50
E. coli DH5 alpha P3 (third triplicate) -2364.333 3563.444 1.000 -15728.50 10999.83
E. coli DH5 alpha Positive control (first triplicate) -19559.333* 3563.444 .000 -32923.50 -6195.17
E. coli DH5 alpha Positive control (second triplicate) -20381.333* 3563.444 .000 -33745.50 -7017.17
E. coli DH5 alpha Positive control (third triplicate) -20455.000* 3563.444 .000 -33819.17 -7090.83
E. coli DH5 alpha negative control (second triplicate) 599.333 3563.444 1.000 -12764.83 13963.50
E. coli DH5 alpha negative control (third triplicate) 205.000 3563.444 1.000 -13159.17 13569.17
E. coli DH5 alpha negative control (second triplicate) E. coli DH5 alpha (first triplicate) 1480.667 3563.444 1.000 -11883.50 14844.83
E. coli DH5 alpha (second triplicate) 1148.667 3563.444 1.000 -12215.50 14512.83
E. coli DH5 alpha (third triplicate) 1493.667 3563.444 1.000 -11870.50 14857.83
E. coli DH5 alpha P1 (first triplicate) -73745.333* 3563.444 .000 -87109.50 -60381.17
E. coli DH5 alpha P1 (second triplicate) -66165.333* 3563.444 .000 -79529.50 -52801.17
E. coli DH5 alpha P1 (third triplicate) -64638.333* 3563.444 .000 -78002.50 -51274.17
E. coli DH5 alpha P2 (first triplicate) -28969.333* 3563.444 .000 -42333.50 -15605.17
E. coli DH5 alpha P2 (second triplicate) -39413.000* 3563.444 .000 -52777.17 -26048.83
E. coli DH5 alpha P2 (third triplicate) -33736.333* 3563.444 .000 -47100.50 -20372.17
E. coli DH5 alpha P3 (first triplicate) -2969.000 3563.444 1.000 -16333.17 10395.17
E. coli DH5 alpha P3 (second triplicate) -4341.000 3563.444 .998 -17705.17 9023.17
E. coli DH5 alpha P3 (third triplicate) -2963.667 3563.444 1.000 -16327.83 10400.50
E. coli DH5 alpha Positive control (first triplicate) -20158.667* 3563.444 .000 -33522.83 -6794.50
E. coli DH5 alpha Positive control (second triplicate) -20980.667* 3563.444 .000 -34344.83 -7616.50
E. coli DH5 alpha Positive control (third triplicate) -21054.333* 3563.444 .000 -34418.50 -7690.17
E. coli DH5 alpha negative control (first triplicate) -599.333 3563.444 1.000 -13963.50 12764.83
E. coli DH5 alpha negative control (third triplicate) -394.333 3563.444 1.000 -13758.50 12969.83
E. coli DH5 alpha negative control (third triplicate) E. coli DH5 alpha (first triplicate) 1875.000 3563.444 1.000 -11489.17 15239.17
E. coli DH5 alpha (second triplicate) 1543.000 3563.444 1.000 -11821.17 14907.17
E. coli DH5 alpha (third triplicate) 1888.000 3563.444 1.000 -11476.17 15252.17
E. coli DH5 alpha P1 (first triplicate) -73351.000* 3563.444 .000 -86715.17 -59986.83
E. coli DH5 alpha P1 (second triplicate) -65771.000* 3563.444 .000 -79135.17 -52406.83
E. coli DH5 alpha P1 (third triplicate) -64244.000* 3563.444 .000 -77608.17 -50879.83
E. coli DH5 alpha P2 (first triplicate) -28575.000* 3563.444 .000 -41939.17 -15210.83
E. coli DH5 alpha P2 (second triplicate) -39018.667* 3563.444 .000 -52382.83 -25654.50
E. coli DH5 alpha P2 (third triplicate) -33342.000* 3563.444 .000 -46706.17 -19977.83
E. coli DH5 alpha P3 (first triplicate) -2574.667 3563.444 1.000 -15938.83 10789.50
E. coli DH5 alpha P3 (second triplicate) -3946.667 3563.444 .999 -17310.83 9417.50
E. coli DH5 alpha P3 (third triplicate) -2569.333 3563.444 1.000 -15933.50 10794.83
E. coli DH5 alpha Positive control (first triplicate) -19764.333* 3563.444 .000 -33128.50 -6400.17
E. coli DH5 alpha Positive control (second triplicate) -20586.333* 3563.444 .000 -33950.50 -7222.17
E. coli DH5 alpha Positive control (third triplicate) -20660.000* 3563.444 .000 -34024.17 -7295.83
E. coli DH5 alpha negative control (first triplicate) -205.000 3563.444 1.000 -13569.17 13159.17
E. coli DH5 alpha negative control (second triplicate) 394.333 3563.444 1.000 -12969.83 13758.50
*. The mean difference is significant at the 0.05 level.