Team:NTU-LIHPAO-Taiwan/Basic Part

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• Notes
  • Preparation of Competent Cells (E.coli)
  • DNA Dissolution
  • Agarose Gel Electrophoresis
  • Gel Extraction
  • DNA Ligation
  • Plasmid DNA Extraction Using Alkaline Lysis Method (E.coli)
  • Transformation (E.coli)

DNA Ligation

Promoter :

Components	Volume (μL)
Insert	x
Vector	y
Ligation High	1
Total	7

1. Gently mix the solution by pipetting up and down
2. Incubate
   1. at 16℃ for 2 hours, or
   2. at 37℃ for 1 hour, or
   3. at 4℃ overnight
3. Proceed with bacterial transformation

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