Team:Leicester/Measurement

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Overview

As a team we decided that we would do some extra work during our time doing the iGEM project this such that we entered to take part in the Interlab Measurement study. The study requires us to look at the fluorescence of given gene circuits made up of parts found in the 2015 Distribution Kits.

The study requires producing 3 devices each consisting of a promoter sequence and Green Fluorescence Protein (GFP) intermediate. Each of the devices are shown below:

  • Device 1 - J23101 + I13504
  • Device 2 - J23106 + I13504
  • Device 3 - J23117 + I13504

As part of the Interlab study, we then did extra credit where we had get biological and technical replicates of each device. They have been defined as such:

  • Biological Replicate -

  • Technical Replicate -

Methodology

In order to get the

Results

Using the Omega Fluostar Fluormeter, we were able to look at the fluorescence of the different devices. It was found that the

Discussion and Difficulties