Team:EPF Lausanne/Part Collection

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

OUR PART COLLECTION

E. coli.

dCas9-ω

dCa9s-ω is our main tool to build our project in E. coli. This catalytically dead Cas9 fused to the RNA polymerase ω subunit can act as an activator or as an inhibitor when provided the right guide RNA.

BBa_K1723000

PAM rich URS J23117

This promoter is composed of the weak J23117 promoter, BBa_J23117, fused to a PAM rich upstream regulatory region (URS) that is needed for dCas9 to bind as dCas9 cannot bind without a PAM sequence.

BBa_TRUCMUCH

sgRNA Z0

the gRNA Z0 inhibit PAM rich URS J23117 promoter when in complex with dCas9-ω.

BBa_TRUCMUCH

sgRNA Z4

the gRNA Z4 activates PAM rich URS J23117 promoter when in complex with dCas9-ω.

BBa_TRUCMUCH

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits