Team:SZMS 15 Shenzhen/interlab
Our Interlab Study
We have created three differently linked devices, each with three biological replicates and measured their OD and fluorescence intensity. We had three technical replicates for each biological replicates.
Data:
A 96 flat bottom black, clear bottom polystyrol plate is used to contain our bacteria during measurements. The distribution of or devices is shown below.
The measurements and the calibrations are shown below.
Mode: Absorbance
Wavelength: 600nm
Number of Flashes: 25
Settle Time: 10ms
Temperature: 24.7 °C
Mode: Absorbance
Wavelength: 600nm
Number of Flashes: 25
Settle Time: 10ms
Temperature: 24.7 °C
The Fluorescence intensity is measured with the data and calibrations shown below.
Mode: Fluorescence Top Reading
Excitation Wavelength: 488nm
Emission Wavelength: 510nm
Excitation Bandwidth: 5nm
Emission Bandwidth: 5nm
Gain: 100 Manual
Number of Flashes: 50
Flash frequency: 400Hz
Integration time: 20µs
Lag Time: 0µs
Settle Time: 0ms
Z-Position (Manual): 20000µm
Temperature: 24.2 °C
Mode: Fluorescence Top Reading
Excitation Wavelength: 488nm
Emission Wavelength: 510nm
Excitation Bandwidth: 5nm
Emission Bandwidth: 5nm
Gain: 100 Manual
Number of Flashes: 50
Flash frequency: 400Hz
Integration time: 20µs
Lag Time: 0µs
Settle Time: 0ms
Z-Position (Manual): 20000µm
Temperature: 24.2 °C
The final result is presented below:
The unit is a.u.
Processing the data:
1. We obtained 9 technical replicates of liquid culture, so we got the average of fluorescence of the liquid culture .Then we subtracted the average number from the data of fluorescence of the other samples.
2. We divided the data by the OD600 measurement to get that of fluorescence released by a unit of each device.
3. We calculated the standard deviation of each device.
4. We omitted the number after the decimal point of all the data.
Notice:
1. Because the number we subtracted from the data of fluorescence was the average of that released by liquid culture, some data became below zero which we presented as "/" in the charts below.
2. Because of our own problems, we did not do the "extra credit work" of J23117 + I13504.
The unit is a.u.
Processing the data:
1. We obtained 9 technical replicates of liquid culture, so we got the average of fluorescence of the liquid culture .Then we subtracted the average number from the data of fluorescence of the other samples.
2. We divided the data by the OD600 measurement to get that of fluorescence released by a unit of each device.
3. We calculated the standard deviation of each device.
4. We omitted the number after the decimal point of all the data.
Notice:
1. Because the number we subtracted from the data of fluorescence was the average of that released by liquid culture, some data became below zero which we presented as "/" in the charts below.
2. Because of our own problems, we did not do the "extra credit work" of J23117 + I13504.
