Team:LZU-China/chz safety
We have taken safety courses before so the entire group members know how to protect ourselves in experiments. As for environment, some of the masses hesitate it may cause genetic pollution to the environment, but it should be noticed that the gene modified E.coli we construct cannot express our target gene unless it is stimulated by the pollutant PNP.
| Species | Strain | Risk Group | Disease risk to humans? If so, which disease | |
|---|---|---|---|---|
| 1 | E. coliK 12 | DH5 alpha | 1 | Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys. However, E. coli K-12 is not considered a human or animal pathogen nor is it toxicogenic. Any concerns for E. coli K-12 in terms of health considerations are mitigated by its poor ability to colonize the colon and establish infections. |
| 2 | Shewenella Onedensis | MR1 | 1 | No, S. oneidensis MR-1 belongs to a class of bacteria known as "Dissimilatory Metal-Reducing Bacteria (DMRB)" because of their ability to couple metal reduction with their metabolism. It cannot cause diseases |
| Part number | Where did us get the physical DNA for this part | What species does this part originally come from? | Natural function of part | What is the Risk Group of the species? | How will you use it? | |
|---|---|---|---|---|---|---|
| 1 | pSB1C3 | From Li Xiangkai's Lab, Lanzhou University | Escherichia coli K-12 | Vector for Escherichia coli | 1 |
|
| 2 | K1523101 | From distributed kit of iGEM 2013 BBA_K381001 |
Escherichia coli K-12 | Detector of Nitrate | 1 | 1. Strain used in anode of MFC 2. Detector of PNP 3. Riboflavin producing |
| 3 | pET-28(a) + | From Li Xiangkai's Lab, Lanzhou University | Vector for Escherichia coli | Vector for Escherichia coli | 1 | Vector for E.coli to express BBA_K1523101 |
| 4 | BBa_K1523008 | Lanzhou University | Escherichia coli | Chromate reduction |
1 | 1. Strain used in anode of MFC 2. Chromate reduction in anode |
| 5 | BBa_K1523021 | Lanzhou University | Pseudomonas fluorescens | Chromate reduction |
1 | 1. Strain used in cathode of MFC 2. Other organic pollutant reduction in cathode |
| 6 | BBa_K1523022 | Lanzhou University | Pseudomonas fluorescens | Chromate reduction | 1 | 1. Strain used in cathode of MFC |
| 7 | BBa_K1523023 | Lanzhou University | Pseudomonas fluorescens | Chromate reduction |
1 | 1. Strain used in cathode of MFC 2. Other organic pollutant reduction in cathode |
| 8 | BBa_K1523025 | Lanzhou University | Pseudomonas putida | Chromate reduction | 1 | 1. Strain used in anode of MFC 2. Chromate reduction in anode |