Team:Bordeaux/Results

IGEM Bordeaux 2015

Project Results

Why choosing crdS, crdA and crdC genes ?

✵ crdS gene codes the Curdlan synthase. ✵ crdA gene codes a protein which assists translocation of nascent polymer across cytoplasmic membrane. ✵ crdC gene codes a protein which assists translocation of nascent polymer across the periplasm.

crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in Agrobacterium sp.ATCC31749.

We tried to work on these three genes. However, amplification attempts by PCR were unsuccessful for crdA and crdC genes. So, in a first time, we focused on cloning crdS gene only.


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