Team:Bordeaux/Results

IGEM Bordeaux 2015

Project Results

Why choosing crdS, crdA and crdC genes ?

crdS gene codes the Curdlan synthase.
crdA gene codes a protein which assists translocation of nascent polymer across cytoplasmic membrane.
crdC gene codes a protein which assists translocation of nascent polymer across the periplasm.
crdA, crdC, crdS genes occupy a contiguous 4,948-bp region in Agrobacterium sp. ATCC31749.

We tried to work on these three genes. However, amplification attempts by PCR were unsuccessful for crdA and crdC genes.So, in a first time, we focused on cloning crdS gene only.



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