Team:Paris Saclay/Notebook/August/13
Contents
Thursday 13th August
Lab Work
Plasmid extraction
by Pauline
Biobricks:
- BBa_R0051 (2nd send)
- BBa_K1707011 #3 (x2)
- BBa_K1707012 #2 (x2)
- BBa_K1707033 #1 and #2
- BBa_K1707031 #1 and #2
- BBa_K1707016 #1 and #2
With Macherey-Nagel Extraction kit (BBa_K1707011 #1 and #2 are assembled in the purification column) (BBa_K1707012 #1 and #2 are assembled in the purification column)
Digestion
by Coralie
Biobricks:
- BBa_K1707011
- BBa_K1707012
- BBa_K1707016 #1 and #2
- BBa_K1707031 #1 and #2
- BBa_K1707033 #1 and #2
- BBa_R0051
Mixes:
- BBa_K1707011 and BBa_K1707012
- 1 µL XbaI
- 2 µL PstI
- 2 µL FastDigest Buffer 10x
- 1 µL H2O
- 15 µL plasmid
- BBa_K1707016 #1 and #2 and BBa_K1707031 #1 and #2
- 1 µL XbaI
- 2 µL PstI
- 2 µL FastDigest Buffer 10x
- 11 µL H2O
- 5 µL plasmid
- BBa_K1707033 #1 and #2
- 1 µL XbaI
- 2 µL PstI
- 2 µL FastDigest Buffer 10x
- 6 µL H2O
- 10 µL plasmid
- BBa_R0051
- 1 µL SpeI
- 2 µL PstI
- 2 µL Tango Buffer 10x
- 5 µL H2O
- 10 µL plasmid
Incubation 1h30, 37°C
Electrophoresis
by Pauline
Agarose gel, 1%, Migration 90V
Biobricks:
- Verification:
- BBa_K1707016 #1 and #2
- BBa_K1707031 #1 and #2
- Purification:
- BBa_K1707011 #3
- BBa_K1707012 #2
- BBa_K1707033 #1 and #2
- BBa_R0051 (2nd send)
We can cut all purification bands. For the verification, we can say that BBa_K1707016 #1 and #2 are OK, but not BBa_K1707031 #1 or #2.
We made a stock of BBa_K1707016 #1 and #2 and BBa_K1707033 #1 and #2.
Purification
by Pauline
Biobricks:
- BBa_K1707011 #3
- BBa_K1707012 #2
- BBa_K1707033 #1 and #2
- BBa_R0051 (2nd send)
With Macherey-Nagel Purification kit
New culture
by Pauline
Biobrick: BBa-K1707031 We put 4 new clones in 5mL LB + 5µL Chloramphenicol
Soil experiment
by Coralie, Ibtissam, Johan and Claire
Member present:
- Instructors: Claire
- Students: Coralie and Pauline