Team:TU Dresden/Notebook/LabJournal
Lab Journal
Week 1: (20-26 July)
- Plasmids were received (T18, ZHER2, LZ-T18, LZ-T25 and HER2 codon) and diluted. Also two pSB1C3 plasmids from iGEM containing RBS (BBa_E0020) and CFP (BBa_E0020) were diluted.
- Preparation of E. coli GB05 and set cultures overnight.
- Electroporation of the cells with the plasmids.
- Transfected cells were streaked out on chloramphenicol (Cm) and kanamycin (kan) plates.
- Colonies were picked and mixed with Cm and kan. These were stored in the fridge.
- Streaking out colonies from DHM1, BTH101 and K12 plates.
Week 2 (27 July-2 August)
- A miniprep was done with the seven cultures with the plasmids. A glycerol stock of each culture was saved.
- The concentration of the plasmids was measured using the nanodrop.
- A digestion of the plasmids was done and a gel was run.
Week 3 (3-9 August)
Week 4 (10-16 August)
Week 5 ()
Week 6 ()
Week 7 ()