Team:HSNU-TAIPEI/projectcopper

ProjectCopper

Introduction

  1. Why we detect Copper?

    Copper is a compulsory ingredient in respiratory pigment, and was found in more and more protein and enzyme. On 1847, Harless found that copper has a great action in Molluscs, and on 1878 Frederig first separated copper from protein contamination in blood of octopus, and he named it as ceraloplasim. On 1928,Hart’s report said that copper is a compulsory Element for manual.

    According to study, since copper can effect the absorption of iron, lack of copper will cause Lenkoryfe abnormal, Neutropenia, Osteoporosis and growth retardation, or damage human’s immune system.

  2. The harm of Copper

    Too much copper will cause harm of human body. It will result in irritation of nose and throat when inhale high concentration of copper. Also it will lead to liver and kidney's damage when intaking high concentration of copper. Copper excession will cause toxicity, including nausea,queasiness, vomit and dirrahea. Other severe symptom like hematuria, jaundice, and urine oligonucleotide may also happen on human beings. Copper poisoning could lead to hemolysis, decrease of hemoglobin and increase of serum lactate dehydrogenase.

  3. Taiwanese regulation
    • 飲用水 1.0 ppm
    • Cooking oil 0.4 ppm
    • Egg 5 ppm
    • Drinks 5 ppm
  4. National regulation
    • 美國環保署要求飲用水中銅濃度不能超過1.3 mg/L。
    • 美國農業部建議八歲以上的人們每天准許含量為900 mg。
    • 美國職業安全及健康管理局(OSHA)要求工作場所中，空氣裡銅的含量不能超過0.1 mg/m3以及含銅的塵土不能超過0.1 mg/m3。

Circuit Design

Just like detecting cadmium , we use the same principle on copper. First, we use the CopA Promoter , which can detect copper and more sensitive than CueO Promoter. When it detects that there is copper in oil, then it will activate the translation reverse.

Result

  1. Whether Copper can enter e.coli or not
    1. Method
    2. Result
    3. Discussion
  2. Whether e.coli is alive in the poisons, condition or not
    1. Method
    2. Results
    3. Discussion
  3. The relation between the concentration of Copper and illumination of RFP
    1. Method
    2. Results
    3. Discussion