Team:Aalto-Helsinki/Practice project

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Practice project: Testing RBSs

Introduction

In synthetic biology it is essential to produce an enormous amount of foreign compounds in a host. One aspect of this is constructing a synthetic genome and choosing the best compatible parts to have enough yield of a desired product. The more product is formed when host cells are big and physically stable.

According to Ceroni et al. (citation), strong ribosomal binding sites (RBSs) affect cell growth and eventually lower the yield of a wanted protein compared to weaker RBSs. The reason behind this is that the strong RBSs reduce significantly the translation of endogenous mRNAs which are needed to cell growth. A ribosomal binding site is a location in an mRNA which a ribosome recognizes and binds to initiating translation. The RBSs is defined by efficiency which they bind to ribosomes. Thus the strong RBSs binds more efficiently than the medium or the weak RBSs.

Before we started our actual project of producing propane in the lab, we thought that it would be interesting to test if weaker RBSs actually improve a protein yield. And we also could get some practice of lab procedures.

Table 1: Our construct parts to test RBSs.

Practice project: Testing RBSs

Introduction

Table 1: Our construct parts to test RBSs.