Team:Paris Saclay/Notebook/August/17

Monday 17th August

Lab Work

Ligation

by Pauline

• BBa_K1707031: BBa_K1707004 and BBa_R0040
  • 12,3 µL BBa_K1707004
  • 2,5 µL BBa_R0040
  • 2 µL Ligase
  • 2 µL Buffer
  • 1,2 µL H2O

Incubation 3h, 4°C

Plasmid extraction

by Pauline

BIobricks:

• BBa_K1707035 #1 and #2
• BBa_K1707036 #1 and #2

With Sylvie Lautru's Protocol

New culture

by Pauline

Biobricks:

• BBa_K1707022 #1 and #2
• BBa_K1707023 #1 and #2
• BBa_K1707034 #1 and #2

We put 2 new clones in 5mL LB + 5µL Chloramphenicol

Transformation

by Pauline

Biobricks:

• BBa_K1707031 (08/17/2015)

Transplant

by Pauline

Biobrick: BBa_K1707031 (08/11/2015) We transplant 9 clones on a LB plate + Cm divided on 9.

Inoculation

by Pauline

3 strains: 1320; 1693; 1696

in 5mL LB with appropriate antibiotic

Member present:

• Instructors: Claire
• Students: Pauline

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