Team:Valencia UPV/Notebook

iGEM 2015 | Valencia UPV

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Valencia UPV Team

Be patient, we are under construction

• Abstract

Notebook

5 June 2015

We had 2 cultures from the last day, corresponding to other 2 colonies of ligation.

Agrobacterium culture of promoter less: Luciferase + Renilla

Minipreps

Digestion with BamHI and EcoRV

PIF6 + PhyB; Ω1	Etr8 CMV+Bxb1_T35S; α1
1µL (GB892) PIF; α1	1µL 1097 (Etr8 CMV) pUPD2
1µL (GB88E) PhyB; α2	1µL Bxb1; pUPD2
1µL Ω1	1µL Tnos PuPD
1.2µL Buffer ligase	1µL α1
1µL Bsmb1	5.8µL H2O
6.8µL H2O

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