Team:Birkbeck/table S18

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Birkbeck iGEM

The Owligos are the first-ever team entered into the international Genetically Engineered Machine (iGEM) Competition by Birkbeck, University of London. We’re a varied group of students who reflect the diversity and unique character of our institution: many of us have chosen science as a second career, having already spent some time in full-time work. For most of us, this has meant making our way through a degree while continuing to work full-time. Hopefully this kind of dedication will help us successfully navigate our way through our iGEM project.

Project Aim

Our project aims to create a new diagnostic solution that will be low-tech and cost-effective enough to allow its usage in deprived and remote communities. We’re attempting to engineer a bacteriophage lambda chassis to change its host affinity, while simultaneously adding a marker that will facilitate easy detection of a target bacterial pathogen in patient samples.

To demonstrate this approach as a proof of concept for the competition, we plan to change this affinity between different strains of E.coli; however, ultimately we hope to demonstrate that this principle could also be applied to alter the phage’s host range to other bacterial species. We could then provide a modular system capable of diagnosing a range of diseases. Of course, we haven’t chosen a simple goal. But as Birkbeck pioneers, we are determined to prove ourselves by making our project a success. We can’t wait to present the results of our work at the Giant Jamboree in September!




fluorescence growth curve 440-580 minutes.htm

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Multiple Comparisons table of Culture Fluorescence Between 440 & 580 minutes
Fluorescence
Tukey HSD
(I) Grouping (J) Grouping Mean Difference (I-J) Std. Error Sig. 95% Confidence Interval
Lower Bound Upper Bound
E. coli DH5α (440 mins)
E. coli DH5α Positive Control (440 mins) -13646.111 6875.026 .984 -39943.11 12650.89
E. coli DH5α P1- (440 mins) -36323.000* 6875.026 .000 -62620.00 -10026.00
E. coli DH5α P2- (440 mins) -24263.222 6875.026 .123 -50560.22 2033.78
E. coli DH5α (460 mins)
E. coli DH5α Positive Control (460 mins) -12765.025 6917.862 .995 -39225.87 13695.82
E. coli DH5α P1- (460 mins) -33210.178* 6700.947 .001 -58841.32 -7579.03
E. coli DH5α P2- (440 mins) -22996.844 6700.947 .160 -48627.99 2634.30
E. coli DH5α (480 mins)
E. coli DH5α Positive Control (480 mins) -13456.575 6917.862 .988 -39917.42 13004.27
E. coli DH5α P1- (480 mins) -33080.533* 6700.947 .001 -58711.68 -7449.39
E. coli DH5α P2- (480 mins) -24418.422 6700.947 .088 -50049.57 1212.72
E. coli DH5α (500 mins)
E. coli DH5α Positive Control (500 mins) -14065.675 6917.862 .978 -40526.52 12395.17
E. coli DH5α P1- (500 mins) -32383.744* 6700.947 .001 -58014.89 -6752.60
E. coli DH5α P2- (500 mins) -26174.744* 6700.947 .038 -51805.89 -543.60
E. coli DH5α (520 mins)
E. coli DH5α Positive Control (520 mins) -15093.650 6917.862 .948 -41554.49 11367.19
E. coli DH5α P1- (520 mins) -34200.289* 6700.947 .000 -59831.43 -8569.15
E. coli DH5α P2- (520 mins) -28390.844* 6700.947 .012 -54021.99 -2759.70
E. coli DH5α (540 mins)
E. coli DH5α Positive Control (540 mins) -16161.575 6917.862 .893 -42622.42 10299.27
E. coli DH5α P1- (540 mins) -37957.700* 6700.947 .000 -63588.84 -12326.56
E. coli DH5α P2- (540 mins) -29835.367* 6700.947 .005 -55466.51 -4204.22
E. coli DH5α (560 mins)
E. coli DH5α Positive Control (560 mins) -17711.525 6917.862 .764 -44172.37 8749.32
E. coli DH5α P1- (560 mins) -42176.400* 6700.947 .000 -67807.54 -16545.26
E. coli DH5α P2- (560 mins) -31172.400* 6700.947 .002 -56803.54 -5541.26
E. coli DH5α (580 mins)
E. coli DH5α Positive Control (580 mins) -18522.625 6917.862 .677 -44983.47 7938.22
E. coli DH5α P1- (580 mins) -43568.167* 6700.947 .000 -69199.31 -17937.02
E. coli DH5α P2- (580 mins) -33529.167* 6700.947 .000 -59160.31 -7898.02
E. coli DH5α Positive Control (440 mins)
E. coli DH5α P1- (440 mins) -22676.889 6875.026 .224 -48973.89 3620.11
E. coli DH5α P2- (440 mins) -10617.111 6875.026 1.000 -36914.11 15679.89
E. coli DH5α Positive Control (460 mins)
E. coli DH5α P1- (460 mins) -20445.153 7086.615 .511 -47551.48 6661.17
E. coli DH5α P2- (460 mins) -10379.375 7086.615 1.000 -37485.70 16726.95
E. coli DH5α Positive Control (480 mins)
E. coli DH5α P1- (480 mins) -19623.958 7086.615 .604 -46730.28 7482.37
E. coli DH5α P2- (480 mins) -10961.847 7086.615 1.000 -38068.17 16144.48
E. coli DH5α Positive Control (500 mins)
E. coli DH5α P1- (500 mins) -18318.069 7086.615 .746 -45424.40 8788.26
E. coli DH5α P2- (500 mins) -12109.069 7086.615 .998 -39215.40 14997.26
E. coli DH5α Positive Control (520 mins)
E. coli DH5α P1- (520 mins) -19106.639 7086.615 .663 -46212.96 7999.69
E. coli DH5α P2- (520 mins) -13297.194 7086.615 .993 -40403.52 13809.13
E. coli DH5α Positive Control (540 mins)
E. coli DH5α P1- (540 mins) -21796.125 7086.615 .364 -48902.45 5310.20
E. coli DH5α P2- (540 mins) -13673.792 7086.615 .989 -40780.12 13432.53
E. coli DH5α Positive Control (560 mins)
E. coli DH5α P1- (560 mins) -24464.875 7086.615 .151 -51571.20 2641.45
E. coli DH5α P2- (560 mins) -13460.875 7086.615 .992 -40567.20 13645.45
E. coli DH5α Positive Control (580 mins)
E. coli DH5α P1- (580 mins) -25045.542 7086.615 .121 -52151.87 2060.78
E. coli DH5α P2- (580 mins) -15006.542 7086.615 .963 -42112.87 12099.78
E. coli DH5α P1- (440 mins)
E. coli DH5α P2- (440 mins) 12059.778 6875.026 .998 -14237.22 38356.78
E. coli DH5α P1- (460 mins)
E. coli DH5α P2- (460 mins) 10065.778 6875.026 1.000 -16231.22 36362.78
E. coli DH5α P1- (480 mins)
E. coli DH5α P2- (480 mins) 8662.111 6875.026 1.000 -17634.89 34959.11
E. coli DH5α P1- (500 mins)
E. coli DH5α P2- (500 mins) 6209.000 6875.026 1.000 -20088.00 32506.00
E. coli DH5α P1- (520 mins)
E. coli DH5α P2- (520 mins) 5809.444 6875.026 1.000 -20487.55 32106.44
E. coli DH5α P1- (540 mins)
E. coli DH5α P2- (540 mins) 8122.333 6875.026 1.000 -18174.67 34419.33
E. coli DH5α P1- (560 mins)
E. coli DH5α P2- (560 mins) 11004.000 6875.026 1.000 -15293.00 37301.00
E. coli DH5α P1- (580 mins)
E. coli DH5α P2- (580 mins) 10039.000 6875.026 1.000 -16258.00 36336.00