Team:HUST-China/Background

Team:HUST-China:Background







Background



A live eukaryotic cell based auto-cementation kit



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With the rapid growth of economy in China, many environmental problems come along. In northern China, the Inner Mongolia grassland is facing increasingly severe desertification[1]; in Hangzhou Bay and Pearl River Delta where cross-sea bridges were built, the loose soil at the base constantly troubles the minds of the constructors and security departments[2]; along the coast of the South China Sea[3], marine ecology damages such as the recession of coral reefs caused by overfishing also becomes a world-concern problem. However, a vital technology may be the solution to all of these problems above—the reinforcement technology. The reinforcement of land and sea soil can efficiently hold the water on the grassland, strengthen the basal soil of cross-sea bridges and restore the marine ecology environment. Therefore, we set our project theme on combining the reinforcement technology with synthesis biology.

Picture 1:Hangzhou Bay Bridge piers far lightmaps


Picture 2:Hangzhou Bay Bridge Piers close-shots


Picture 3:The city facing the danger of desertification


Picture 4:The devastated coral reefs


The existing reinforcement technologies usually tend to chemical or physical approaches and employ synthetic polymers as the curing agent. These polymers are toxic and nondegradable in the environment, thus will bring contamination and damages for a long time. Comparing with the traditional methods, the microbial reinforcement technology [6], an emerging environment-friendly technology, shows its superiority. The principle of microbial reinforcement is adding urea and calcium chloride solution to the soil with bacillus strain in it. Under the induction of calcium ion, the strain will produce calcium carbonate which will deposit and cement the soil to perform the reinforcing function. Our project is inspired by the microbial reinforcement technology and we further developed it in the light of synthesis biology. We developed a reinforcement kit in order to apply our method in many similar problems. The kit includes the control system, the supporting system and the flocculation system. Our engineering yeast is named Euk.cement, i.e. the eukaryote used to cement. Nowadays, the coral reef recession has rung alarm to the whole world. There are over 10% of coral reefs that are damaged and another 30% to 80% under threat of human activity. According to the statistics released by The Nature Conservancy, the recession speed of coral reefs is increasing and 70% of coral reefs will disappear in 50 years. Under this stressful circumstance, we hope that our engineering yeast can be used in the construction of artificial reefs and make an effort in restoring marine environment and saving the endangered coral reefs. Due to the seriousness of the reefs problem, we focus on the issue of artificial reefs to restore marine ecosystems. With the reinforcement kit, we use specific parts to collect and reinforce the floating sand in the sea and form artificial reefs and use Yarrowia lipolytica as the chassis to suit the demand of marine environment. To know what Euk.Cement is exactly, please turn to description.



Reference

[1] Ash R F, Edmonds R L. China's land resources, environment and agricultural production[J]. The China Quarterly, 1998, 156: 836-879. [2]Diamond Jared “Collapse.” Pp.364-5.Penguins Books,2005 [3]Andrea Ding-Kemp.Enr.construction.com. Retrieved 2010-07-05. [4] Murray N J, Clemens R S, Phinn S R, et al. Tracking the rapid loss of tidal wetlands in the Yellow Sea[J]. Frontiers in Ecology and the Environment, 2014, 12(5): 267-272. [5]MacKinnon, J.; Verkuil, Y.I.; Murray, N.J. (2012), Asian Coastal Wetlands [6]Jroundi F, Gómez-Suaga P, Jimenez-Lopez C, et al. Stone-isolated carbonatogenic bacteria as inoculants in bioconsolidation treatments for historical limestone[J]. Science of the total environment, 2012, 425: 89-98.