Team:Goettingen/Experiments



LB Medium

Plasmid Extraction - using peqGOLD Plasmid Miniprep Kit I (PEQLAB Technologies)

Plasmid Extraction - using QIAprep Spin Miniprep Kit (QIAGEN)

Blunt End Ligation in pJET1.2 vector –Clone JET PCR Cloning Kit–Thermo Scientific

TOPO® Cloning protocol usingChampion™ pET Directional TOPO® Expression Kits. Thermo Fisher Scientific

PCR product purification using QIAquick® PCR Purification Kit (QIAGEN).

Plasmid transformation into Chemically Competent E. coli.

Preparation of competent E.coli cells

Esterase activity test:

PCR Gel extraction, peqGOLD Gel Extraction Kit

Electroporation of BL21 cells with pJET_RFP

Media preparation for cellulase activity screening

Experiments & Protocols

Describe the experiments, research and protocols you used in your iGEM project.

What should this page contain?
  • Protocols
  • Experiments
  • Documentation of the development of your project

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