Team:Technion Israel/Lab Notebook/Protocols

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Lab Notebook

Human Practices

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Overview Protocols

Overview Bacillus E.Coli NADPH Comb Modeling

Design

• 

  • Gel Extraction

  • Sigma-Aldrich Miniprep

  • Phusion PCR

  • Electroporation

    E.coli

    B.Subtilis

  • Transformation

    E.coli

    B.Subtilis

  • Colony PCR

    B. Subtilis

  • Preparing Competent E.coli

  • DNA Gel Electrophoresis

  • Preparation of DNA for Sequencing

• 

  • Comparison of Lysis Procedures

  • Bacillus Secretion Verification

• 

  • Primer Dilution

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