Team:NTU-LIHPAO-Taiwan/Modeling/Pathways

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Pathways

Bacteria Distribution

After orally ingested, certain amounts of modified probiotic L. casei can survive under acidic condition when passing through the stomach. Owning to gastric acid, bile acid and digestive enzymes, the presence of these bacteria in the intestinal tract only last for a limited time, and then are washed out in faeces.[1] During the process of transition and temporary colonization, CPP-PYY complexes are produced and exert the following effect.

In the first part of Modeling, bacteria distribution in the small intestine was investigated. Data from Y. K. Lee et al. showed bacteria number against time adhered on the duodenum, jejunum, and ileum in mouse after orogastric intubation of 10⁹ L. casei, which were listed in Table 1. [2] The ratio (%) of bacteria number to total ingested number were also indicated.

By summing up all the data, we constructed the continuous L. casei cell number and ratio distribution against time with curve fitting in MATLAB, as shown in Figure 1 and Figure 2. However, experimental data from suicide mechanism should be incorporated to complete the simulation for the survival of L. casei in the small intestine, here we hypothesized 4 days for bacteria survival. Therefore, the time-changing tendency could be utilized for further calculation.

Fig.2-1 Nisin Selection

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