Team:Paris Saclay/Notebook/August/24

Monday 24th August

Lab Work

New culture

by Audrey

Biobrick: BBa_K1707037 #1 to #10

Electrophoresis

by Audrey

Biobricks:

• BBa_K1707004 #5 (digested by SpeI + EcoRI)
• BBa_K1707022 (digested by XbaI + PstI)

Agarose gel 1%, Migration 90V

We don't have any gel picture because the gel broked. We should do this electrophoresis again.

Digestion

by Audrey

Biobricks:

• BBa_K1707004 #5 and BBa_K1707023 #1:
  • 10 µL plasmid
  • 1 µL SpeI
  • 1 µL EcoRI
  • 2 µL Buffer 2 NEB
  • 6 µL H2O

• BBa_K1707004 #5 and BBa_K1707023 #1:
  • 10 µL plasmid
  • 1 µL SpeI
  • 1 µL PstI
  • 2 µL Buffer 2 NEB
  • 6 µL H2O

• BBa_K1707022 #1:
  • 15 µL plasmid
  • 1 µL XbaI
  • 1 µL PstI
  • 2 µL Buffer FastDigest 10x
  • 1 µL H2O

• BBa_R0040:
  • 20 µL plasmid
  • 1 µL XbaI
  • 1 µL EcoRI
  • 3 µL Buffer FasrDigest 10x
  • 5 µL H2O

Incubation 1h30, 37°C

Member present:

• Instructors: Claire
• Students: Audrey

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