Team:TCU Taiwan/Result/vitro
Aug. 6th ,2015
| • * PCR signiferin |
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2% gel. The PCR product of signiferin should be 100~200 bp. As the result, there is band between 100~200 bp on sample 2 so we consider it is correct. |
Aug. 15th ,2015
| • signiferin TA plasmid purification |
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0.8% gel. The size of TA plasmid with signiferin insert should be 2000~3000 bp. In addition, plasmid will become sorpercoil so it will run faster. Therefore, we consider these are the correct plasmid. |
Aug. 16th ,2015
| • pQE60 plasmid digest with enzyme BamH I and Nco I biobrick digest with enzyme EcoRI and Pst I |
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2% gel. The size of signiferin insert should be 100~20 bp. In addition, the signiferin insert in biobrick should be a little bigger than in pQE60. As the result, these two are all the correct sample we need. |
Aug. 21st ,2015
| • PCR Epi-1 insert |
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2% gel. It is the correct size that Epi-1 PCR product have band between 200~300 bp. But, there is also band on blank. We consider it was polluted when we add reagent. |
| • plasmid purification of pQE60 vector with signiferin insert |
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0.8% gel. The size of our plasmid should be 3000 to 4000. As the result, these samples are the plasmid we need. |
Aug. 22rd ,2015
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• Plasmid purification of pQE60 plasmid with signiferin insert
• Plasmid purification of backbone with signiferin insert |
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0.8％ gel. The size of signiferin biobrick should be 2000~3000 bp, and the size of pQE60 plasmid with signiferin should be 3000~4000 bp. In addition, the plasmid will become surpercoil so it will run faster. Therefore, they are all correct pasmid. |
Aug. 23rd ,2015
| • Enzyme digestion to check the clone of biobrick with signiferin |
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2％ gel. The size of signiferin insert should be 100 to 200 bp. There is band on the sample 2 of enzyme digest product. As the result, we can make sure that the signiferin has been ligated in backbone. |
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| Contact us tcutaiwan@gmail.com No.701, Sec. 3, Zhongyang Rd. Hualien 97004, Taiwan |