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• Own Device
  We built our own simplified and affordable detection device. Check out the step by step instructions !
• New iGEM Backbone
  We provided a new backbone for protein overexpression meeting all the iGEM standards.
  See how we epanded the iGEM registry!
• Human Practice
  What do people think about the DiaCHIP and systems based on synthetic biology?
  Check out the results of our survey and discussions !
• Modeling
  Modeling the generation of proteins during cell-free expression as well as their diffusion in our system helped us obtaining better results. Get more information here.
• Blood Serum Analysis
  We successfully detected tetanus antibodies in human blood serum and measured binding of GFP antibodies to cell-free expressed GFP.
  Visit our results page !
• Diagnostics today
  Nowadays, medical diagnosis is mainly based on huge amounts of time-consuming and costly serological tests. Read more about the issues we tackled !
• The DiaCHIP
  Simultaneously screening for hundreds of diseases within a few hours?
  Find out about the DiaCHIP's potential to revolutionize future diagnostics !
• Our System
  Generating a protein array on demand via cell-free expression and detecting antigen-antibody interactions label-free and in real-time: The principle behind the DiaCHIP !

DiaCHIP - Enlightening Diagnostics

In modern medicine, fast detection and differentiation of diseases is a crucial and fundamental task. Typical ELISA-based assays are time-consuming and expensive. We propose an advanced procedure for the simultaneous detection of various diseases in a fast and inexpensive manner, the DiaCHIP. Our approach is based on the interaction of antibodies with their respective antigens. Different antigens are immobilized on a protein array generated by cell-free protein expression, using the corresponding DNA array as a template. Placed in a microfluidic chamber, the protein array is incubated with a patient’s blood sample. The interaction between an antibody in the sample and the corresponding immobilized antigen results in a local change of the optical thickness of the surface. This change can be detected using a label-free and real-time measurement technology called iRIf (imaging Reflectometric Interference). Offering simultaneous screening for several diseases, our DiaCHIP has strong potential to improve future diagnostics.