Team:Northeastern Boston/BasicParts

Northeastern

• Home
• Team
• Project
Parts
Basic & Composite
Expression Plasmid
Notebook
Protocols
Workflow
• Attributions
Human Practices
Outreach
Open Source
Jurassic Wet Lab
• Safety
• Measurement

BASIC & COMPOSITE PARTS

Hygromycin B Resistance Cassette

Recognizing that the iGEM repository lacks a modular selection marker for microalgae, Northeastern submits a Hygamycin B resistance cassette. This composite part was cloned from pOpt_mVenus_Hyg and adapted to the iGEM standards. As a complete resistance cassette, this part is expected to confer Hygromycin B resistance to any Chlamydomonas reinhardtii transfected with this part.

This part contains intron 2 of RBCs2 after a fusion HSP70A-RBCs2 promoter for greater expression.[1]