Team:NCTU Formosa/Notebook

Lab note
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

PCR
transform 		2

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 31
Sun. Mon. Tue. Wed. Thu. Fri. Set.
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 15

Transformation of
GBP[FadL-GBP]
Cultivation of
OmpA[OmpA+pSB1K3] Kr 		16

Cultivation of
GBP[FadL-GBP]
Plasmid purification of
OmpA[OmpA+pSB1K3] Kr
Digestion of
OmpA[OmpA+pSB1K3] Kr (EcoRI and PstI) 		17

Plasmid purification of
GBP[FadL-GBP]
Digestion of
GBP[FadL-GBP] (EcoRI and PstI)
Electrophoresis of
digested product:
GBP[FadL-GBP] (EcoRI and PstI) 		18

Transformation of
ligated product:
GBP[FadL-GBP+pSB1C3] Cr
19

Cultivation of
ligated product: GBP[FadL-GBP] Cr
PCR product: Terminator[J61048] 		20

 21

Cultivation of
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
OmpA[OmpA+pSB1C3] Cr
GBP[FadL-GBP+pSB1C3] Cr 		22

Plasmid purification of
GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar
OmpA[OmpA+pSB1C3] Cr
GBP[FadL-GBP+PSB1C3] Cr
Digestion of
GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
GBP[FadL-GBP] Cr (EcoRI and PstI)
Electrophoresis of
digested product:
GFP[J23101+B0034+E0040+J61048] Ar (EcoRI and PstI)
OmpA[OmpA] Cr (EcoRI and PstI)
GBP[FadL-GBP] Cr (EcoRI and PstI) 		23

Digestion of OmpA[OmpA] Cr (EcoRI and SpeI)
GBP[FadL-GBP] Cr (EcoRI and SpeI)
Electrophoresis of
digested product:
OmpA[OmpA] Cr (EcoRI and SpeI)
GBP[FadL-GBP] Cr (EcoRI and SpeI) 		24

Digestion of
OmpA[OmpA] Cr (XbaI and PstI)
GBP[FadL-GBP] Cr (XbaI and PstI) 		25

Electrophoresis of
digested product:
OmpA[OmpA] Cr (XbaI and PstI)
GBP[FadL-GBP] Cr (XbaI and PstI)
Digestion of
RBS[B0034] Ar (XbaI and PstI)
backbone[pSB1A3] Ar (EcoRI and PstI)
Terminator[J61048] Ar (XbaI and PstI)
Electrophoresis of
digested product:
RBS[B0034] Ar (XbaI and PstI)
backbone[pSB1A3] Ar (EcoRI and PstI)
Terminator[J61048] Ar (XbaI and PstI)
Ligation of
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
GBP[GBP+B0034+pSB1K3] Kr
OmpA[OmpA+J61048+pSB1K3] Kr
26

Ligation of
RFP[E1010+B0034+pSB1K3] Kr
Transformation
of ligated product:
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
GBP[FadL-GBP+B0034+pSB1K3] Kr
OmpA[OmpA+J61048+pSB1K3] Kr
Electrophoresis of
digested product:
RFP[E1010+J61048+pSB1K3] Kr
[J23101+B0034] 		27

Digestion of
Terminator[J61048] Ar (XbaI and PstI)
PCR of
ligated product:
OmpA[J23101+B0034+OmpA+pSB1A3] Ar
OmpA[OmpA+J61048+pSB1K3] Kr 		28

Electrophoresis of
RFP[E1010+J61048+pSB1K3] Kr
OmpA[J23101+B0034+OmpA+pSB1A3] Ar Transformation of
ligated product:
GBP[B0034+FadL-GBP+pSB1A3] Ar
OmpA[OmpA+J61048+pSB1K3] Kr PCR of
ligated product
OmpA[J23101+B0034+OmpA] Ar 		29

Cultivation of
RFP[E1010+J61048+pSB1K3] Kr PCR of
OmpA[J23101+B0034+OmpA] Ar
Electrophoresis of
OmpA[J23101+B0034+OmpA] Ar Ligation of
GBP[FadL-GBP+J61048+pSB1K3] Kr 		30

Plasmid purification of
RFP[E1010+J61048+pSB1K3] Kr Ligation of
OmpA[J23101+B0034+OmpA] Ar PCR of
OmpA[J23101+B0034+OmpA] Ar Transformation of
mini: Terminator[J61048]
ligated product: OmpA[J23101+B0034+OmpA] Ar
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

PCR of Transformation product: mini: Terminator[J61048] ligated product: OmpA[J23101+B0034+OmpA] Ar GBP[B0034+FadL-GBP+pSB1A3] Ar Electrophoresis of mini: Terminator[J61048] ligated product: OmpA[J23101+B0034+OmpA] Ar 		2
3

Ligation of GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar 		4

Ligation of GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar 5/4 Transformation off ligated products: GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar Digestion of RFP[E1010+J61048] (Kr) (XbaI and PstI) PCR of ligated products: GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar Electrophoresis of PCR products: GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar 		5

Electrophoresis of digested products: RFP[E1010+J61048] (Kr) (XbaI and PstI) OmpA[J23101+B0034+OmpA] Ar (EcoRI and PstI) GBP[FadL-GBP+J61048] Kr (EcoRI and PstI) PCR of OmpA[J23101+B0034+OmpA+pSB1A3] Ar Cultivation of PCR product: RFP[E1010+J61048+pSB1K3] Kr GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar 		6

Plasmid purification of RFP[E1010+J61048+pSB1K3] Kr GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar Digestion of GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI) Cultivation of PCR product: OmpA[J23101+B0034+OmpA+pSB1A3] Ar PCR of backbone: [pSB1C3] Cr [pSB1A3] Ar [pSB1K3] Kr Electrophoresis of PCR products: OmpA[J23101+B0034+OmpA+pSB1A3] Ar digested products: GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI) 		7

Digestion of RFP[E1010+J61048+pSB1K3] Kr (EcoRI and PstI) RFP[E1010+J61048+pSB1K3] Kr (XbaI and PstI) [pSB1C3] Cr (EcoRI and PstI) [pSB1A3] Ar (EcoRI and PstI) [pSB1K3] Kr (EcoRI and PstI) OmpA[J23101+B0034+OmpA+pSB1A3] Ar (EcoRI and PstI) Plasmid purification of OmpA[J23101+B0034+OmpA+pSB1A3] Ar [pSB1K3] Kr Electrophoresis of digested products: RFP[E1010+J61048+pSB1K3] Kr (EcoRI and PstI) ???? RFP[E1010+J61048+pSB1K3] Kr (XbaI and PstI) [pSB1C3] Cr (EcoRI and PstI) [pSB1A3] Ar (EcoRI and PstI) [pSB1K3] Kr (EcoRI and PstI) Ligation of RFP[B0034+E1010+J61048+pSB1C3] Cr 		8

Electrophoresis of digested products: OmpA[J23101+B0034+OmpA] Ar (EcoRI and PstI) Transformation off ligated products: RFP[B0034+E1010+J61048+pSB1C3] Cr PCR of GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar Electrophoresis of PCR products: GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar Transformation off GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar [B0030] Ar [J23110] Ar 		9

PCR of GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar [B0030] Ar [J23110] Ar Electrophoresis of PCR products: GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar [B0030] Ar [J23110] Ar Cultivation of [B0030] Ar [J23110] Ar Ligation of GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar
10

Electrophoresis of [B0030] Ar Cultivation of [B0030] Ar Plasmid purification of [J23110] Ar [B0030] Ar GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar Digestion of [B0030] Ar (EcoRI and SpeI) [J23110] Ar (EcoRI and SpeI) GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI) GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI) Electrophoresis of digested products: [B0030] Ar (EcoRI and SpeI) GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI) GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI) 		11

Electrophoresis of digested products: [J23110] Ar (EcoRI and SpeI) Ligation of RFP[B0030+E1010+J61048+pSB1C3] Cr GFP[B0030+E0040+J61048+pSB1K3] Kr Transformation off RFP[B0030+E1010+J61048+pSB1C3] Cr GFP[B0030+E0040+J61048+pSB1K3] Kr PCR of RFP[B0030+E1010+J61048+pSB1C3] Cr GFP[B0030+E0040+J61048+pSB1K3] Kr 		12

PCR of ligated products: RFP[B0030+E1010+J61048+pSB1C3] Cr Cultivation of PCR products: RFP[B0030+E1010+J61048+pSB1C3] Cr Plasmid purification of RFP[B0030+E1010+J61048+pSB1C3] Cr Digestion of RFP[B0030+E1010+J61048] Cr (EcoRI and SpeI) RFP[B0030+E1010+J61048] Cr (EcoRI and PstI) Electrophoresis of digested products: RFP[B0030+E1010+J61048] Cr (EcoRI and SpeI) RFP[B0030+E1010+J61048] Cr (EcoRI and PstI) PCR products: RFP[B0030+E1010+J61048+pSB1C3] Cr 		13

Ligation of [J23110+B0034+pSB1K3] Kr 		14

Cultivation of OmpA[J23101+B0034+OmpA+pSB1A3] Ar Transformation off [J23110+B0034+pSB1K3] Kr Electrophoresis of digested products: GBP[J23101+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI) GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI) [pSB1K3] Kr (EcoRI and PstI) 		15

Digestion of GBP[B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI) Ligation of GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr 		16

Transformation off GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr Electrophoresis of GBP[B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI) GBP[B0034+FadL-GBP+pSB1A3] Ar (XbaI and PstI) Ligation of GBP[B0034+FadL-GBP+pSB1C3] Cr Transformation off [J23110+B0034+pSB1K3] Kr
17

Ligation of [J23110+B0034+pSB1K3] Kr GBP[J23110+B0034+FadL-GBP+pSB1K3] Kr PCR of GBP[B0034+FadL-GBP+pSB1C3] Cr GBP[J23110+B0034+FadL-GBP+pSB1K3] Kr Electrophoresis of PCR products: GBP[B0034+FadL-GBP+pSB1C3] Cr GBP[J23110+B0034+FadL-GBP+pSB1K3] Kr 		18

Cultivation of GBP[B0034+FadL-GBP+pSB1C3] Cr Plasmid purification of GBP[B0034+FadL-GBP+pSB1C3] Cr Digestion of GBP[B0034+FadL-GBP] Cr (XbaI and PstI) RFP[B0030+E1010+J61048] Cr (XbaI and PstI) GFP[B0030+E0040+J61048] Transformation off [J23110+B0034+pSB1K3] Kr GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr GFP[B0030+E0040+J61048] 		19

Ligation of GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr PCR of GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr Electrophoresis of digested products: RFP[B0030+E1010+J61048] Cr (XbaI and PstI) GBP[B0034+FadL-GBP] Cr (XbaI and PstI) PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr Transformation off scFv-B[scFv-B+pSB1K3] Kr scFv-C[scFv-C+pSB1K3] Kr GFP[B0030+E0040+J61048] Cultivation of GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr 		20

Cultivation of OmpA[J23101+B0034+OmpA] Ar scFv-B[scFv-B+pSB1K3] Kr scFv-C[scFv-C+pSB1K3] Kr Plasmid purification of OmpA[J23101+B0034+OmpA] Ar GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr scFv-B[scFv-B+pSB1K3] Kr scFv-C[scFv-C+pSB1K3] Kr Digestion of OmpA[J23101+B0034+OmpA] Ar (EcoRI and Pst1) GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr (EcoRI and PstI) OmpA[J23101+B0034+OmpA] Ar (NcoI and PstI) scFv-B[scFv-B] Ar (EcoRI and PstI) scFv-C[scFv-C] Ar (EcoRI and PstI) Ligation of GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr Transformation off [J23110+B0034+pSB1K3] Kr [pSB3C5] 抗性 Electrophoresis of OmpA[J23101+B0034+OmpA] Ar (EcoRI and PstI) 		21

Digestion of scFv-B[scFv-B] Ar (EcoRI and PstI) scFv-C[scFv-C] Ar (EcoRI and PstI) Electrophoresis of OmpA[J23101+B0034+OmpA] Ar (NcoI and PstI) scFv-B[scFv-B] Ar (EcoRI and PstI) scFv-C[scFv-C] Ar (EcoRI and PstI) Ligation of scFv-B[scFv-B+pSB1C3] Cr scFv-C[scFv-C+pSB1C3] Cr scFv-B[scFv-B+pSB1K3] Kr scFv-C[scFv-C+pSB1K3] Kr 		22

Cultivation of [pSB3C5] Cr [J23110+B0034+pSB1K3] Kr Transformation off GBP[J23110+B0034+GBP] Ar PCR of scFv-B[scFv-B+pSB1C3] Cr scFv-C[scFv-C+pSB1C3] Cr scFv-B[scFv-B+pSB1K3] Kr scFv-C[scFv-C+pSB1K3] Kr Electrophoresis of scFv-B[scFv-B+pSB1C3] Cr scFv-C[scFv-C+pSB1C3] Cr scFv-B[scFv-B+pSB1K3] Kr scFv-C[scFv-C+pSB1K3] Kr Ligation of scFv-B[scFv-B+pSB1A3] Ar scFv-C[scFv-C+pSB1A3] Ar Transform of scFv-B[scFv-B+pSB1A3] Ar scFv-C[scFv-C+pSB1A3] Ar 		23

Plasmid purification of [pSB3C5] Cr [J23110+B0034+pSB1K3] Kr Digestion of [pSB3C5] Cr (EcoRI and PstI) [J23110+B0034+pSB1K3] Kr (EcoRI and PstI) PCR of GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr Transformation off scFv-B[scFv-B+pSB1A3] Ar scFv-C[scFv-C+pSB1A3] Ar
24

Electrophoresis of digested products: [pSB3C5] Cr (EcoRI and PstI) [J23110+B0034+pSB1K3] Kr (EcoRI and PstI) Cultivation of PCR products: GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr scFv-B[scFv-B+pSB1C3] Cr scFv-C[scFv-C+pSB1A3] Ar Plasmid purification of scFv-B[scFv-B+pSB1A3] Ar scFv-C[scFv-C+pSB1A3] Ar GFP[B0030+E0040+J61048] Digestion of [J23101+B0034] Kr (EcoRI and SpeI) Ligation of OmpA[J23101+B0034+OmpA+pSB3C5] Cr PCR of GBP[J23110+B0034+GBP] Ar by two pArts Electrophoresis of GBP[J23110+B0034+GBP] Ar 		25

Transformation off OmpA[J23101+B0034+OmpA+pSB3C5] Cr Cultivation of GBP[J23110+B0034+FadL-GBP] Ar Digestion of GBP[B003+FadL-GBP] Cr (XbaI and PstI) scFv-B[scFv-B+pSB1C3] Cr (EcoRI and PstI) scFv-C[scFv-C+pSB1A3] Ar (EcoRI and PstI) GFP[B0030+E0040+J61048] (XbaI and PstI) Electrophoresis of GFP[B0030+E0040+J61048] scFv-B[scFv-B+pSB1C3] Cr (EcoRI and PstI) scFv-C[scFv-C+pSB1A3] Ar (EcoRI and PstI) GFP[B0030+E0040+J61048] (XbaI and PstI) Cultivation of scFv-B[scFv-B+pSB1C3] Cr (EcoRI and PstI) scFv-C[scFv-C+pSB1A3] Ar (EcoRI and PstI) GFP[B0030+E0040+J61048] (XbaI and PstI) 		26

Ligation of OmpA[J23110+B0034+OmpA+pSB1A3] Ar GBP[J23110+B0034+GBP+pSB1A3] Ar Gel extraction of [J23110] Ar (SpeI and PstI) Plasmid purification of GBP[J23110+B0034+GBP] Ar by two pArts Digestion of GBP[J23110+B0034+GBP] Ar by two pArts (EcoRI and SpeI) 		27

PCR of [J23101+B0034+OmpA+pSB3C5] Electrophoresis of GBP[J23110+B0034+GBP] Ar by two pArts (EcoRI and SpeI) GBP[B0034+FadL-GBP] Cr (XbaI and PstI) [J23110] Ar (SpeI and PstI) 		28

Ligation of scFv-B[scFv-B+pSB1A3] Ar scFv-C[scFv-C+pSB1A3] Ar GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] 抗性?? scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar Transform of scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] scFv-B[scFv-B+pSB1A3] Ar scFv-C[scFv-C+pSB1A3] Ar PCR of [J23101+B003+OmpA+pSB3C5] + primer(EX-OmpA and OmpA-S) OmpA[J23101+B0034+OmpA+pSB3C5] Cr Plasmid purification in gel slices of PCR products: OmpA[EX-OmpA-S] Gel extraction of OmpA[OmpA] (EcoRI and SpeI) Transformation off OmpA[J23101+B0034+OmpA+pSB3C5] 		29

Plasmid purification of [J23110] Ar [pSB1C3] Digestion of [J23110] Ar (EcoRI and SpeI) [pSB1C3] (EcoRI and SpeI) Ligation of scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar [J23110+B0034+pSB1K3] Kr Electrophoresis of [J23110] Ar (EcoRI and SpeI) [pSB1C3] (EcoRI and SpeI) Transform of scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar [J23110+B0034+pSB1K3] Kr GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr 		30

PCR of scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar [J23110+B0034+pSB1K3] Kr GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr
31

Cultivation of [J23110+B0034] Kr GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar Plasmid purification of [J23110+B0034] Kr GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar Digestion of [J23110+B0034] Kr (EcoRI and PstI) GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar (EcoRI and PstI) [J23110+B0034] (EcoRI and SpeI) Kr [J23101+B0034] (EcoRI and SpeI) Kr OmpA[OmpA] Cr (EcoRI and PstI) [pSB1K3] (EcoRI and PstI) Electrophoresis of [J23110+B0034] Kr (EcoRI and PstI) GFP[J23110+B0034+E0040+J61048+pSB1C3] Cr (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+pSB1A3] Ar (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+pSB1A3] Ar (EcoRI and PstI) Ligation of OmpA[OmpA+pSB1C3] Cr
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

Electrophoresis of [J23110+B0034] (EcoRI and SpeI) Kr [J23101+B0034] (EcoRI and SpeI) Kr OmpA[OmpA] (NcoI and PstI) GFP[K1489002+E0040] Kr Digestion of [pSB1C3] (EcoRI and PstI) [pSB1A3] (EcoRI and PstI) OmpA[OmpA] (EcoRI and NcoI) [pSB3C5] (EcoRI and PstI) Ligation of GFP[J23101+B0034+K1489002+E0040+pSB3C5] (Cr) GFP[J23110+B0034+K1489002+E0040+pSB3C5] (Cr) Electrophoresis of digested product: [pSB1C3] (EcoRI and PstI) [pSB1A3] (EcoRI and PstI) OmpA[OmpA] (EcoRI and NcoI) Cultivation of BFP[OmpA+K592100] PCR of GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr Electrophoresis of GBP[J23101+B0034+FadL-GBP+pSB1K3] Kr 		2

PCR of OmpA[EX-OmpA-S+pSB1C3] Cr Electrophoresis of OmpA[EX-OmpA-S+pSB1C3] Cr OmpA[OmpA] (EcoRI and NcoI) Ligation of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E1010+J61048+pSB1K3] Kr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr Digestion of [pSB1A3] (EcoRI and NcoI) Electrophoresis of digested products: [pSB1A3] (EcoRI and NcoI) 		3

Ligation of OmpA[OmpA+psB1C3] Cr [J23110+B0034+pSB1K3] Kr Transform of OmpA[OmpA+psB1C3] Cr [J23110+B0034+pSB1K3] Kr Digestion of BFP[OmpA+ K592100] (NcoI and PstI) OmpA[OmpA] (NcoI and PstI) 		4

Cultivation of [J23110+pSB1A3] Ar Transform of [pSB1C3] Digestion of [pSB1C3] (EcoRI and PstI) Ligation of OmpA[OmpA+pSB1C3] Cr OmpA[J23101+B0034+OmpA+pSB3C5] Cr Transform of [B0034+pSB1A3] Ar 		5

Transform of OmpA[J23101+B0034+OmpA+pSB3C5] Cr Plasmid purification of [J23110+pSb1A3] Ar Digestion of [J23110+pSb1A3] Ar PCR of [J23110+pSb1A3] Ar BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr Transform of OmpA[OmpA+pSB1C3] Cr Electrophoresis of [pSB1C3] [B0034+pSB1A3] Ar BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr 		6

Cultivation of [pSB1C3] Digestion of [pSB1C3] (EcoRI and SpeI)
7

Gel extraction of OmpA[EX-OmpA-S] 抗性 [pSB1C3] (EcoRI and SpeI) Gel extraction of [pSB1C3] (EcoRI and SpeI) Transform of GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar [pSB1A3] OmpA[J23101+B0034+OmpA] Ar Ligation of OmpA[OmpA+pSB1C3] Cr Cultivation of [pSB1C3] 		8

Transform of OmpA[OmpA+pSB1C3] Cr [pSB3K3] [pSB6A1] Plasmid purification of [pSB1C3] Digestion of OmpA[EX-OmpA-S] (EcoRI and SpeI) Electrophoresis of [pSB1C3] (EcoRI and SpeI) Transform of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr 		9

Ligation of OmpA[OmpA+pSB1C3] Cr PCR of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr Cultivation of BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr Plasmid purification of [pSB3K3] [pSB6A1] Digestion of [pSB3K3] (EcoRI and PstI) [pSB6A1] (EcoRI and PstI) 		10

Transform of OmpA[OmpA+pSB1C3] Cr Plasmid purification of [J23101+B0034+OmpA+K592100+pSB3C5] Cr Digestion of [J23101+B0034+OmpA+K592100] Cr (EcoRI and PstI) Electrophoresis of [pSB3K3] (EcoRI and PstI) [pSB6A1] (EcoRI and PstI) [pSB1A3] (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B] (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C] (EcoRI and PstI) Transform of [J23110+B0034] Kr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Ligation of scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr Electrophoresis of [pSB3K3] BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr 		11

Transform of scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr [J23110+B0034+pSB1C3] Cr Ligation of [J23110+B0034+pSB1K3] Kr [J23110+B0034+pSB1C3] Cr PCR of OmpA[OmpA+pSB1C3] Cr scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr Electrophoresis of OmpA[OmpA+pSB1C3] Cr scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr Cultivation of OmpA[OmpA+pSB1C3] Cr 		12

PCR of [J23110+B0034+pSB1C3] Cr [B0034+pSB1A3] Ar Plasmid purification of OmpA[OmpA+pSB1C3] Cr Ligation of [J23110+B0034+pSB1C3] Cr Transform of [J23110+B0034+pSB1C3] Cr 		13

Electrophoresis of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI)
14

SDS-page of [pSB1C3] scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr (EcoRI and PstI) [pSB3C5] scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr scFv-B[OmpA+scFv-B] scFv-C[OmpA+scFv-C] Electrophoresis of [J23110] [B0034+FadL-GBP+B0030+E1010+J61048] 抗性 (XbaI and PstI) Ligation of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3K3] Cr GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Cr Digestion of OmpA[OmpA+pSB1C3] Cr (EcoRI and SpeI) OmpA[OmpA+pSB1C3] Cr (EcoRI and PstI) Transform of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar Ligation of GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar Transform of GBP[FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar 		15

 16

Transform of [pSB3C5] 		17

Cultivation of [pSB3C5] 		18

Plasmid purification of [pSB3C5] Digestion of [pSB3C5]( EcoRI and PstI) Electrophoresis of [pSB3C5]( EcoRI and PstI) Ligation of BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr 		19

Transform of BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr 		20

PCR of BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr Electrophoresis of BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr
21

 22

 23

PCR of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr Cultivation of [pSB3C5] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar BFP[OmpA+K592100] 抗性 scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] [psB6A1] GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar [pSB1A3] GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] Cr Ligation of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		24

Plasmid purification of [pSB3C5] 		25

Cultivation of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr Transform of [J23101+B0034+pSB1K3] Kr 		26

 27

Digestion of OmpA[OmpA] Cr (EcoRI and PstI) Electrophoresis of GBP[FadL-GBP] 抗性 (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Ar (EcoRI and PstI) OmpA[OmpA] Cr (EcoRI and PstI)
28

Cultivation of BFP[J23101+B0034+OmpA+K592100+pSB1A3] Ar GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] Cr GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr [pSB6A1] [pSB3C5] [pSB1C3] [pSB1A3] scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr Electrophoresis of [J23101+B0034] Kr GBP[FadL-GBP] 抗性 (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Ar (EcoRI and PstI) OmpA[OmpA] Cr (EcoRI and PstI) BFP[J23101+B0034+OmpA+K592100] Ar (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Cr (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Cr (EcoRI and PstI) [pSB6A1] (EcoRI and PstI) OmpA[OmpA] Cr (XbaI and PstI) [E0040] Ar (XbaI and PstI) Ligation of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr [B0034+E0040+pSB1K3] Kr GBP[FadL-GBP+pSB6A1] Ar GBP[FadL-GBP+pSB1C3] Cr GBP[FadL-GBP+pSB3C5] Cr Digestion of BFP[J23101+B0034+OmpA+K592100] Cr (XbaI and PstI) SDS-page of [pSB1A3] [pSB1C3] BFP[J23101+B0034+OmpA+K592100+pSB1A3] Ar scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr [pSB3C5] [pSB6A1] GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB3C5] Cr GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar Transform of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar GBP[FadL-GBP+pSB1C3] Cr GBP[FadL-GBP+pSB3C5] Cr BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr 		29

PCR of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar GBP[FadL-GBP+pSB1C3] Cr GBP[FadL-GBP+pSB3C5] Cr BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr Electrophoresis of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		30

Electrophoresis of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

Cultivation of [pSB6A1] scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar Transform of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr Digestion of GBP[]FadL-GBP] 抗性 (EcoRI and PstI) 		2

Digestion of [J23110] Ar (SpeI and PstI) Plasmid purification in gel slices of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar Digestion of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) Electrophoresis of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		3

Gel extraction of [J23110] Ar (SpeI and PstI) [pSB6A1] (EcoRI and PstI) Transform of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr Electrophoresis of GBP[FadL-GBP] (EcoRI and Pstl) Ligation of GBP[FadL-GBP+pSB3C5] Cr GBP[FadL-GBP+pSB1C3] Cr GBP[FadL-GBP+pSB6A1] Ar 		4

Transform of GBP[FadL-GBP+pSB3C5] Cr GBP[FadL-GBP+pSB1C3] Cr GBP[FadL-GBP+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr Electrophoresis of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr Cultivation of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr [J23101+B0034+pSB1K3] Kr
5

Plasmid purification of [J23101+B0034+pSB1K3] Kr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr 		6

Cultivation of [pSB1C3] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr [pSB6A1] scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar [pSB1A3] GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar 		7

Digestion of [J23101+B0034+pSB1K3] Kr (EcoRI and PstI) SDS-page of [pSB1C3] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr [pSB6A1] scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar [pSB1A3] GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar Ligation of [J23110+B0034] Ar Electrophoresis of [J23101+B0034+pSB1K3] Kr (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		8

Plasmid purification of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar Transform of [J23110+B0034] Ar BFP[J23101+B0034+OmpA+K592100] scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100] Electrophoresis of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		9

Electrophoresis of scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+scFv-B+B0034+E0040+J61048+pSB1C3] Cr [pSB3C5] BFP[J23101+B0034+OmpA+scFv-B+B0030+e0040+J61048+pSB6A1] Ar Cultivation of scFv-B[J23110+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		10

Plasmid purification of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		11

PCR of [J23110+B0034] Ar Electrophoresis of [J23110+B0034] Ar
12

Cultivation of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar [J23110+B0034] Ar Plasmid purification of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar Digestion of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		13

Plasmid purification of [J23110+B0034] Ar Transform of [R0010] Electrophoresis of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		14

Digestion of [J23110+B0034] Ar (EcoRI and PstI) GBP[FadL-GBP] Kr (XbaI and PstI) OmpA[OmpA] Cr (EcoRI and SpeI) Electrophoresis of [J23110+B0034] Ar (EcoRI and PstI) GBP[FadL-GBP] Kr (XbaI and PstI) OmpA[OmpA] Cr (EcoRI and SpeI) Transform of [R0010] Cr Cultivation of [pSB1C3] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr 		15

Digestion of [pSB6A1] [pSB3C5] Anti-Her 2[Herceptin+E1010] [J23101+B0034+OmpA+scFv-C] [J23101+B0034+OmpA+scFv-B] Cultivation of [pSB6A1] [R0010] PCR of OmpA[OmpA-linker] (EcoRI and PstI) Transform of [J23110+B0034] Kr BFP[K592100+J61048] Kr 		16

Gel extraction of OmpA[OmpA-linker] (EcoRI and PstI) Ligation of OmpA[J23101+B0034+OmpA+pSB3C5] Cr BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr Digestion of OmpA[OmpA-linker] (EcoRI and PstI) Transform of OmpA[J23101+B0034+OmpA+pSB3C5] Cr 		17

Electrophoresis of OmpA[OmpA-linker] (EcoRI and PstI) Anti-Her 2[Herceptin+E1010] (EcoRI and PstI) scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) 		18

Ligation of OmpA[OmpA-linker+pSB1C3] Cr Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
19

Transform of OmpA[OmpA-linker+pSB1C3] Cr Anti-Her 2[Herceptin+E1010+pSB1C3] Cr 		20

 21

 22

 23

 24

Transform of OmpA[OmpA-linker+pSB1C3] Cr Anti-Her 2[Herceptin+E1010+pSB1C3] Cr PCR of OmpA[OmpA-linker+pSB1C3] Cr Anti-Her 2[Herceptin+E1010+pSB1C3] Cr Electrophoresis of OmpA[OmpA-linker+pSB1C3] Cr Anti-Her 2[Herceptin+E1010+pSB1C3] Cr 		25

Cultivation of [R0010] Cr BFP[K592100+J61048] Kr PCR of OmpA[OmpA-linker+pSB1C3] Cr Anti-Her 2[Herceptin+E1010+pSB1C3] Cr Electrophoresis of OmpA[OmpA-linker+pSB1C3] Cr Anti-Her 2[Herceptin+E1010+pSB1C3] Cr
26

Plasmid purification of [R0010] Cr BFP[K592100+J61048] Kr Digestion of [R0010] Cr (EcoRI and SpeI) BFP[K592100+J61048] Kr (EcoRI and PstI) Electrophoresis of [R0010] Cr BFP[K592100+J61048] Kr Ligation of [R0010+B0034+pSB1K3] Kr GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr BFP[B0030+K592100+J61048+pSB1C3] Cr Transform of scFv-C[J23101+B0034+OmpA+scFv-C] Ar Ligation of OmpA[OmpA-linker+pSB1C3] Cr PCR of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048] Cultivation of [pSB1C3] GFP[J23101+B0034+E0040+J61048+pSB1A3] Ar scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr GBP[FadL-GBP+pSB6A1] Ar 		27

PCR of GBP[FadL-GBP] Anti-Her 2[Herceptin+E1010] Gel extraction of GBP[FadL-GBP] Anti-Her 2[Herceptin+E1010] Transform of [R0010+B0034+pSB1K3] GBP[R0010+B0034+FadL-GBP+B0030+J61048+pSB1K3] BFP[B0030+K592100+J61048+pSB1C3] Cr PCR of GBP[J23110+B0034+FadL-GBP+E0040+J61048] Cr Gel extraction of GBP[J23110+B0034+FadL-GBP+E0040+J61048] Cr SDS-page of [pSB1C3] GFP[J23101+B0034+E0040+J61048+pSB1C3] Cr scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr GBP[J23110+B0034+FadL-GBP+E0040+J61048+pSB1C3] Cr GBP[FadL-GBP+pSB6A1] Ar [pSB6A1] 		28

Ligation of [R0010+B0034+pSB1K3] GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] Kr BFP[B0030+K592100+J61048+pSB1C3] Cr GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1C3] Cr GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] Ar PCR of BFP[B0030+K592100+J61048+pSB1C3] Cr [R0010+B0034+pSB1K3] GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB1K3] Kr OmpA[J23110+B0034+OmpA+pSB3C5] Cr Digestion of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI) Electrophoresis of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048] (XbaI and PstI) SDS-page of [pSB1C3] BFP[J23101+B0034+OmpA+K592100+pSB1C3] Cr GFP[J23101+B0034+E0040+J61048+pSB1C3] Cr scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] Cr [pSB6A1] GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] Ar Transform of OmpA[OmpA-linker] Cr 		29

Electrophoresis of OmpA[J23101+B0034+OmpA+pSB3C5] Transform of GBP[J23110+B0034+FadL-GBP+B0030+E0040+J61048+pSB1C3] Cr GBP[R0010+B0034+FadL-GBP+B0030+E0040+J661048+pSB6A1] Ar PCR of BFP[B0034+K592100+J61048+pSB1C3] Cr GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Electrophoresis of BFP[B0034+K592100+J61048+pSB1C3] Cr GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Digestion of Anti-Her 2[Herceptin-own] (EcoRI and PstI) Anti-Her 2[Herceptin-E1010] (EcoRI and PstI) GBP[GBP] (EcoRI and PstI) Ligation of [R0010+B0034+pSB1K3] Kr BFP[B0030+K592100+J61048] Cr GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] Ar GFP[R0010+B0030+E0040+J61048] Ar Electrophoresis of Anti-Her 2[Herceptin-own] (EcoRI and PstI) Anti-Her 2[Herceptin-E1010] (EcoRI and PstI) GBP[GBP] (EcoRI and PstI) [R0010+B0034+pSB1K3] Kr BFP[B0030+K592100+J61048] Cr GBP[R0010+B0034+FadL-GBP+B0034+E0040+J61048] Ar GFP[R0010+B0030+E0040+J61048] Ar 		30

Cultivation of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB1C3] Cr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB1C3] Cr Ligation of GBP[FadL-GBP+pSB1C3]Cr Digestion of Anti-Her 2[Herceptin own] (NcoI and PstI) [pSB1A3] (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) Cultivation of scFv-C[J23101+B0034+OmpA+scFv-C] Ar [J23101+B0034] Kr Digestion of scFv-B[scFv-B] Kr (NcoI and PstI) scFv-C[scFv-C] Kr (NcoI and PstI) OmpA[OmpA] Cr (EcoRI and PstI) Electrophoresis of Anti-Her 2[Herceptin own] (NcoI and PstI) [pSB1A3] (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) Plasmid purification of scFv-C[J23101+B0034+OmpA+scFv-C] Ar [J231-1+B0034] Kr Digestion of scFv-C[J23101+B0034+OmpA+scFv-C] Ar (EcoRI and SpeI) [J231-1+B0034] Kr (EcoRI and SpeI) Anti-Her 2[Herceptin-Own] (NcoI and PstI) 		31

Electrophoresis of scFv-C[J23101+B0034+OmpA+scFv-C] Ar (EcoRI and SpeI) [J231-1+B0034] Kr (EcoRI and SpeI) Anti-Her 2[Herceptin-Own] (NcoI and PstI) Ligation of Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] Ar Transform of Anti-Her 2[J2310+B0034+OmpA+Herceptin-Own+pSB1A3] Ar Electrophoresis of [pSB1C3] (XbaI and PstI) OmpA[OmpA] Cr (EcoRI and PstI) scFv-B[scFv-B] Kr (NcoI and PstI) scFv-C[scFv-C] Kr (NcoI and PstI)
Sun. Mon. Tue. Wed. Thu. Fri. Set.
1

Digestion of Anti-Her 2[Herceptin-Web] (NcoI and PstI) Electrophoresis of Anti-Her 2[Herceptin-Web] (NcoI and PstI)
2

Electrophoresis of [pSB1C3] (XbaI and PstI) OmpA[OmpA] (EcoRI and PstI) scFv-B[scFv-B] Kr (NcoI and PstI) scFv-C[scFv-C] Kr (NcoI and PstI) OmpA[OmpA] (NcoI and PstI) Digestion of BFP[OmpA+K592100] Cr (EcoRI and NcoI) 		3

Transform of [R0010+B0034+pSB1K3] Kr GBP[FadL-GBP+pSB1C3] Cr BFP[B0030+K592100+J61048] Cr Digestion of Anti-Her 2[Herceptin-Web] (NcoI and PstI) Ligation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar 		4

Digestion of BFP[OmpA+K592100] Cr (EcoRI and NcoI) PCR of [R0010+B0034+pSB1K3] Kr BFP[B0030+K592100+J61048] Cr Transform of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar 		5

Digestion of [pSB6A1] (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) PCR of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar Electrophoresis of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar Cultivation of BFP[B0030+K592100+J61048] Cr GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] PCR of GBP[FadL-GBP] Cr BFP[OmpA+K592100] Cr scFv-C[J23101+B0034+OmpA+scFv-C] Ar scFv-C[scFv-C+pSB1C3] Cr scFv-B[scFv-B+oSB1C3] Cr Digestion of scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr (EcoRI and PstI) scFv-B[J23101+B0034+OmpA+scFv-B+pSB3C5] Cr (EcoRI and PstI) Transform of GBP[J23101+B0034+FadL-GBP]Cr scFv-B[scFv-B+pSB1C3] Cr scFv-C[scFv-C+pSB1C3] Cr scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Cr Gel extraction of [pSB6A1] (EcoRI and PstI) [OmpA] (NcoI and PstI) [pSB1C3] (EcoRI and PstI) 		6

Cultivation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar Ligation of Anti-Her 2[OmpA+Herceptin-Web] Cr Anti-Her 2[OmpA+Herceptin-Own] Cr scFv-C[OmpA+scFv-C] Cr scFv-B[OmpA+scFv-B] Cr scFv-C[J23101+B0034+OmpA+scFv-C] Cr scFv-B[J23101+B0034+OmpA+scFv-B] Cr Transform of Anti-Her 2[OmpA+Herceptin-Web] Cr Anti-Her 2[OmpA+Herceptin-Own] Cr scFv-C[OmpA+scFv-C] Cr scFv-B[OmpA+scFv-B] Cr scFv-C[J23101+B0034+OmpA+scFv-C] Cr scFv-B[J23101+B0034+OmpA+scFv-B] Cr [pSB1C3] [pSB1A3] [pSB1K3] Plasmid purification of BFP[B0030+K592100+J61048] Digestion of BFP[B0030+K592100+J61048] (EcoRI and PstI) Cultivation of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Cr Ligation of [R0010+B0034] Kr GBP[GBP[J23101+B0034+FadL-GBP]+pSB1C3] Cr Digestion of [pSB1C3] (XbaI and PstI) Electrophoresis of GBP[J23101+B0034+FadL-GBP] Cr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] Cr scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] Cr scFv-C[scFv-C] Cr scFv-B[scFv-B] Cr Plasmid purification of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar Digestion of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and PstI) Ligation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar Electrophoresis of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar [B0034] [pSB1C3] Cultivation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar Transform of GBP[J23101+B0034+FadL-GBP] Ar Electrophoresis of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040] Cr scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040] Cr [pSB1C3] (XbaI and PstI) [pSB6A1] (XbaI and PstI) [pSB1C3] (XbaI and PstI) Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar Cultivation of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Cr scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Cr GFP[J23101+B0034+E0040+J61048] Ar SDS-page of GFP[J23101+B0034+E0040+J61048] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Ar scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048] Ar [pSB6A1] GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Ar Digestion of [pSB1C3] (XbaI and PstI) Anti-Her 2[Herceptin-Web] (NcoI and PstI) Anti-Her 2[Herceptin-Own] (NcoI and PstI) Ligation of [pSB1C3] (XbaI and PstI) Anti-Her 2[Herceptin-Web] Cr Anti-Her 2[Herceptin-Own] Cr BFP[J23101+B0034+OmpA+K592100] Cr 		7

Plasmid purification of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Cr Digestion of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Cr PCR of scFv-B[J23101+B0034+OmpA+scFv-B] Cr scFv-C[J23101+B0034+OmpA+scFv-C] Cr Electrophoresis of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and PstI) Cultivation of scFv-B[J23101+B0034+OmpA+scFv-B] Cr scFv-C[J23101+B0034+OmpA+scFv-C] Cr Transform of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar Plasmid purification of GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Cr Digestion of GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Cr (EcoRI and PstI) PCR of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar Electrophoresis of scFv-B[J23101+B0034+OmpA+scFv-B] Cr scFv-C[J23101+B0034+OmpA+scFv-C] Cr Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar OmpA[OmpA] (NcoI and PstI) [pSB1C3] (EcoRI and PstI) Ligation of scFv-C[OmpA+scFv-C] Cr scFv-B[OmpA+scFv-B] Cr Anti-Her 2[Herceptin-Own] Cr Anti-Her 2[Herceptin-Web] Cr Anti-Her 2[OmpA+Herceptin-Own] Cr Anti-Her 2[OmpA+Herceptin-Web] C Electrophoresis of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and PstI) Anti-Her 2[Herceptin-Web] (NcoI and PstI) Anti-Her 2[Herceptin-Own] (NcoI and PstI) Transform of scFv-C[OmpA+scFv-C] Cr scFv-B[OmpA+scFv-B] Cr Anti-Her 2[Herceptin-Own] Cr Anti-Her 2[Herceptin-Web] Cr Anti-Her 2[OmpA+Herceptin-Own] Cr Anti-Her 2[OmpA+Herceptin-Web] C BFP[J23101+B0034+OmpA+K592100] Cr 		8

Plasmid purification of scFv-C[J23101+OmpA+scFv-C] Cr scFv-B[J23101+OmpA+scFv-B] Cr Digestion of scFv-C[J23101+OmpA+scFv-C] Cr (EcoRI and PstI) scFv-B[J23101+OmpA+scFv-B] Cr (EcoRI and PstI) PCR of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar Cultivation of [pSB1K3] [pSB1C3] [pSB1A3] PCR of BFP[J23101+B0034+OmpA+K592100] Cr Transform of scFv-C[OmpA+scFv-C] Cr Electrophoresis of BFP[J23101+B0034+OmpA+K592100] Cr scFv-C[J23101+OmpA+scFv-C] Cr (EcoRI and PstI) scFv-B[J23101+OmpA+scFv-B] Cr (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) Digestion of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar (EcoRI and SpeI) OmpA[OmpA] Cr NcoI and PstI OmpA[OmpA+K592100] Cr NcoI and PstI Ligation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Cr Digestion of Anti-Her 2[Herceptin-Own] (EcoRI and PstI) Anti-Her 2[Herceptin-Web] (EcoRI and PstI) Electrophoresis of BFP[J23101+B0034+OmpA+K592100] Cr
9

Cultivation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar Plasmid purification of [pSB1C3] [pSB1K3] [pSB1A3] Digestion of [pSB1C3] (EcoRI and PstI) [pSB1K3] (EcoRI and PstI) [pSB1A3] (EcoRI and PstI) Transform of Anti-Her 2[Herceptin-Own] Cr Anti-Her 2[Herceptin-Web] Cr Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Cr [R0010+B0034] Cr BFP[J23101+B0034+OmpA+K592100] Cr Electrophoresis of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Cr (EcoRI and SpeI) OmpA[OmpA] Cr (NcoI and PstI) Digestion of [pSB1A3] (EcoRI and PstI) [pSB1K3] (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) RFP[B0030+E1010+J61048] Cr (XbaI and PstI) Electrophoresis of scFv-C[OmpA+scFv-C] Kr PCR of GBP[GBp+pSB1C3] Cr Digestion of BFP[B0030+K592100+J61048] Cr (XbaI and PstI) 		10

Electrophoresis of GBP[GBp+pSB1C3] Cr [pSB1A3] (EcoRI and PstI) [pSB1K3] (EcoRI and PstI) [pSB1C3] (EcoRI and PstI) Ligation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web+B0030+E1010+J61048] Kr Cultivation of [R0010+B0034] Cr BFP[B0030+K592100+J61048] Cr scFv-C[OmpA+scFv-C] Kr Digestion of [pSB1C3] (EcoRI and Pstl) Transform of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048+pSB1K3] Kr Gel extraction of [pSB1C3] (EcoRI and PstI) PCR of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Anti-Her 2[Herceptin-Web] Anti-Her 2[Herceptin-Own] 		11

Plasmid purification of [R0010+B0034] Cr scFv-C[OmpA+scFv-C] Kr BFP[OmpA+K592100] Cr Gel extraction of [pSB1C3] (EcoRI and PstI) Digestion of [R0010+B0034] Cr (EcoRI and SpeI) scFv-C[OmpA+scFv-C] Kr (EcoRI and PstI) BFP[OmpA+K592100] Cr (EcoRI and PstI) GBP[J23101+B0034+FadL-GBP] Cr (EcoRI and SpeI) Ligation of Anti-Her 2[Herceptin-Web] Cr PCR of scFv-B[OmpA+scFv-B] Kr Electrophoresis of [R0010+B0034] Cr (EcoRI and SpeI) scFv-C[OmpA+scFv-C] Kr (EcoRI and PstI) BFP[OmpA+K592100] Cr (EcoRI and PstI) GBP[J23101+B0034+FadL-GBP] Cr (EcoRI and SpeI) Ligation of GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar Electrophoresis of scFv-B[OmpA+scFv-B] Kr PCR of scFv-C[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr Transform of GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr 		12

Electrophoresis of scFv-B[OmpA+scFv-B] Kr Transform of Anti-Her 2[Herceptin-Web] Cr Anti-Her 2[Herceptin-Own] Cr Anti-Her 2[J23101+B0034+OmpA+Herceptin-Web] Ar PCR of scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar Digestion of [pSB1C3] (EcoRI and PstI) Electrophoresis of scFv-C[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		13

Transform of GBP[J231010+B0034+FadL-GBP+B0030+E0040+J61048+pSB6A1] Ar Cultivation of [pSB1C3] scFv-B[OmpA-scFv-B] Kr Plasmid purification of [pSB1C3] scFv-B[OmpA-scFv-B] Kr Digestion of [pSB1C3] (EcoRI and PstI) scFv-B[OmpA-scFv-B] Kr (EcoRI and PstI) Electrophoresis of [pSB1C3] (EcoRI and PstI) Ligation of OmpA[R0010+B0034+OmpA+pSB1A3] Ar GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar scFv-B[J23101+B0034+scFv-B+B0030+E0040+J61048+pSB6A1] Ar scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar Electrophoresis of [pSB1C3] (EcoRI and PstI) scFv-B[OmpA-scFv-B] Kr (EcoRI and PstI) 		14

Transform of OmpA[R0010+B0034+OmpA+pSB1A3] Ar GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		15

PCR of OmpA[R0010+B0034+OmpA+pSB1A3] Ar GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar scFv-C[J23101+B0034+scFv-C+B0030+E0040+J61048+pSB6A1] Ar Ligation of scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr BFP[J23101+B0034+OmpA+K592100+pSB3C5] Cr PCR of GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr Transform of scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr Electrophoresis of GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr Electrophoresis of GBP[FadL-GBP] (EcoRI and PstI)
16

PCR of GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Kr Cultivation of OmpA[R0010+B0034+OmpA] Electrophoresis of GBP[R0010+B0034+FadL-GBP+pSB1A3] Kr GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] Kr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Kr 		17

Ligation of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar Transform of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048+pSB6A1] Ar 		18

Plasmid purification of OmpA[R0010+B0034+OmpA+pSB1A3] Ar GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar Digestion of BFP[OmpA+K592100] Cr (EcoRI and NcoI) Anti-Her 2[Herceptin-Own] (NcoI and SpeI) Anti-Her 2[Herceptin-Web] (NcoI and SpeI) Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar (EcoRI and SpeI) BFP[B0030+K592100+J61048] Cr (XbaI and PstI) OmpA[R0010+B0034+OmpA+pSB1A3] Ar (EcoRI and PstI) GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar (EcoRI and PstI) Ligation of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] Cr GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar Transform of Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own+pSB1C3] Cr GBP[R0010+B0034+FadL-GBP+pSB1A3] Ar GBP[J23101+B0034+fadL-GBP+B0030+E0040+J61048] Cr OmpA[R0010+B0034+OmpA+pSB1C3] Cr GBP[R0010+B0034+FadL-GBP+pSB1C3] Cr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E0040+J61048] Ligation of scFv-B[R0010+B0034+OmpA+scFv-B] scFv-C[R0010+B0034+OmpA+scFv-C] Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own] Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web] GBP[R0010+B0034+FadL-GBP+B0030+E0040+J61048] PCR of Anti-Her 2[Herceptin-Web] scFv-B[J23101+B0034+OmpA+scFv-B] scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr Anti-Her 2[Herceptin-Own] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar 		19

Electrophoresis of Anti-Her 2[Herceptin-Web] scFv-B[J23101+B0034+OmpA+scFv-B] scFv-C[J23101+B0034+OmpA+scFv-C+pSB3C5] Cr Anti-Her 2[Herceptin-Own] scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar scFv-C[OmpA+scFv-C] Anti-Her 2[Herceptin-Own] (NcoI and SpeI) Anti-Her 2[Herceptin-Web] (NcoI and SpeI) Anti-Her 2[J23101+B0034+OmpA+Herceptin-Own] Ar (EcoRI and SpeI) BFP[B0030+K592100+J61048] Cr (XbaI and PstI) Transform of GBP[R0010+B034+FadL-GBP] Ar GBP[J23101+B0034+FadL-GBP] Ar GBP[J23101+B0034+FadL-GBP+B0030+E0040+J61048] Cr 		20

PCR of GBP[FadL-GBP] Kr OmpA[OmpA] Cr Gel extraction of OmpA[OmpA] (EcoRI and SpeI) Plasmid purification of Anti-Her 2[Herceptin-Own] Cr scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar PCR of GBP[FadL-GBP] Kr OmpA[R0010+B0034+OmpA+pSB1C3] Cr GBP[R0010+B0034+GBP+pSB1C3] Cr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr GBP[R0010+B0034+GBP+B0030+E0040+J61048] scFv-B[R0010+B0034+OmpA+scFv-B] scFv-C[R0010+B0034+OmpA+scFv-C] Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own] Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web] Digestion of scFv-C[J23101+B0034+OmpA+scFv-C+B0030+E0040+J61048+pSB6A1] Ar (EcoRI and PstI) Anti-Her 2[Herceptin-Own] Cr Electrophoresis of OmpA[R0010+B0034+OmpA+pSB1C3] Cr GBP[R0010+B0034+GBP+pSB1C3] Cr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048] Kr GBP[R0010+B0034+GBP+B0030+E0040+J61048] Ligation of scFv-B[R0010+B0034+OmpA+scFv-B+pSB1K3] Kr scFv-C[R0010+B0034+OmpA+scFv-C+pSB1K3] Kr Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+pSB1K3] Kr Anti-Her 2[R0010+B0034+OmpA+Herceptin-Web+pSB1K3] Kr GBP[R0010+B0034+GBP+B0030+E0040+J61048pSB1A3] Ar scFv-B[J23101+B0034+OmpA+scFv-B+B0030+E1010+J61048+pSB1K3] Kr scFv-B[J23101+B0034+OmpA+scFv-B+B0030+K592100+J61048+pSB1K3] Kr Anti-Her 2[R0010+B0034+OmpA+Herceptin-Own+B0030+E1010+J61048+pSB1K3] Kr 		21

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