Team:Czech Republic/Protocols

Protocols

Purification

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Restriction

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GA

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Soft lithography - PDMS molding

  • Silicon masters
  • 40g PDMS + 4g curing agent
  • curing 80°C/2h

Soft lithography - Bonding PDMS-Glass

Preparation of the substrates

Glass slide
  • Rinse the glass slide with acetone, isopropanol, and deionized water
  • Dry the glass slide with air gun
  • Dehydrate the glass slide on a hot plate at 120°C for 30 minutes
PDMS
  • Slice the PDMS to individual PDMS replicas
  • Drill holes for microfluidic inlets and outlets
  • Clean the PDMS using a scotch tape

Air plasma treatment

  • Position the substrate

Permanent irreversible bonding

  • Place PDMS over glass slide
  • post-bake 80°C/1h

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