Team:NAIT Edmonton/Logbook

Team NAIT 2015

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First Day in Lab!

Our team got the overview of our work space for the summer. We were geared and ready to start the iGEM adventure!

May 4
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Research into Silver Staining

Before beginning a project, it is of great importance to fully understand what it is you are planning to research. Our team focused on understanding the basics and chemistry of silver staining completely.

May 4 to 8
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Formation of Color in Silver Staining

What was the scientific basis of color formation post silver staining?

May 11 to 15
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Spruce Grove High School Lab

Students taking Biology 30 at SGHS came to NAIT today and we were lucky enough to be able to T.A for them.

May 21
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Alberta Innovates and geekStarter

Team NAIT went on a road trip to the University of Calgary!

May 24
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Research Continued...

Getting our wiki set up and looking for sponsorships.

May 25 to 29
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Getting Our Timeline Set Up

Time is ticking! We are already into the second month of the summer!

June 1 to 5
Location

techLife and NAIT Photoshoot


We had a photoshoot with NAIT's techLife magazine. Thank you to Ms. Linda Hoang and Blaise for your patience!

June 11
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Ongoing Research and Practice

Perfecting our silver stain techniques!

June 12
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Sequences Ordered!

First four sequences of our own design ordered from IDT!

June 16
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Met the President!

No, not Obama. We met NAIT's President! Dr. Glenn Felthan and NAIT's VP External and CDO, Mr. George Andrews were most accommodating.

June 18
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Article on Synthetic Biology in Canada

Submitted an article for Team iGEM Amoy China.

June 20
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Plasmid and Sequence Digestion

A plasmid double digestion was performed along with digestion of four of our sequences. The sequences used were Arginine, Lysine, Glutamic Acid and Cysteine samples.

June 22
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Sequences Arrived!

June 24
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Research, Presentations and Practice

"If we knew what it was we were doing, it would not be called research, would it?" - Albert Einstein

June 22 to 25
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Presentation to the Office of Research and Innovation

Today, we were lucky enough to have ORnI hear, assess and critique our preliminary presentation for iGEM. We are so happy to know that we have the full support of so many amazing individuals at the Office of Research and Innovation and at NAIT.

June 26
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Happy Canada Day!

July 1
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Pumping out those Sequences

This week, our team really focused on creating a variety of sequences for testing. Equipped with the knowledge from our literature searches in prior weeks, we crafted each sequence carefully and diligently. As a team, we created and ordered over 25 this week!

June 29 to July 3
Location

And so the cloning begins...

Dr. Marcet helped us get started with cloning! But first, we had to test our enzymes to see if they will properly linearize our plasmid!

July 6
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Expressing our Custom Sequences in our E. coli

Got our sequences inserted (hopefully) and now we want to grow our bacteria!

July 10
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Team BBQ!

We had an awesome Sunday barbecue! The sun was shining bright, we had cold drinks and great company - A perfect day.

July 12
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Maya Monday

Slowly cranking out those animations! Stay tuned!

July 13
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Experiment and Protocols Flowchart

Drafted and published the first version of our experimental protocols and design! You can view it here.

July 16
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Officially Registered!

SO EXCITED!!!!!!!!!

July 20
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AITF and geekStarter Workshop

NAIT is off for the 6 hour drive to visit a workshop hosted by our co-Albertan team, Team Lethbridge!

July 24 to 26
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Team Re-Branding

Today, we stepped away from our original iGEM inspired logo and tried to come up with a new team identity so that we could stand out from the crowd and our team would leave a lasting impression in iGEM.

July 30
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NAIT Photoshoot and Mini-Prep

We had another photo shoot with our institution! Eduardo and Joy managed to escape early, however, because of a plasmid mini-prep.

July 31
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Let's Work Together!

Our team is new to iGEM so we are helping out others as best we can. We've completed many surveys and given feedback to many teams!

August 6
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Title and Abstract.. Submitted!

Today was the due date for the final abstract and title for iGEM's brochures and websites.

August 7
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Officially Argentum!

Received our new logo concepts from our friend and design advisor, Derek Lue. We changed the layout of our Wiki to what you see now!

August 10
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Abbie's Back!

Abbie’s last day working her full-time summer student position as a lab tech for Keyera AEF. Time to get into the lab and work on the project!

August 14
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Go, Team NAIT, GO!

We're getting an overwhelming amount of support from NAIT. We're even getting golf shirts to wear in Boston!

August 17
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Argentum ... Re- IM[Ag]INE 'd

Turns out, we cannot use Argentum as our team name. There is a local company that has trademarked that brand and we do not want to get into any trouble.

August 19
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Watch our Intro Video!

We have a new respect for anyone who has to be in front of the camera as their career.. This less-than-two-minutes video took us a few hours to shoot and edit.

August 20
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iGEM Banner

Final banner design finished and read for sending to iGEM HQ. We cannot wait to see our banner hanging at the Hynes Convention Centre.


August 21
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Collaboration with our New Application Track

As you know, we're a first-time team. We reached out to our Track mates and asked them to help us out.

August 26-28
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Digestion of Sequences

All our sequences (23) were digested and in doing so each were cleaved at specific sites to later ligate with our plasmid pSB1C3.

August 26
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IDT Interview

Turns out, we cannot use Argentum as our team name. There is a local company that has trademarked that brand and we do not want to get into any trouble.

Read more August 27