Team:Bielefeld-CeBiTec

iGEM Bielefeld 2015


Cell-free Sticks - It works on paper.

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Our project

Our team decided to establish cell-free systems ("cell-free protein synthesis" and "plasmid repressor interaction assay"). For demonstrating the extensibility of our systems we worked with biosensors for heavy metals and we construct a new biosensor for date rape drugs. See what we are doing this year.

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Our results

Our aim is to establish a universally usable and cell-free biosensor on paper. We could demonstrate that some of our biosensors can be detected on paper. Take a look how we achieved our goal successfully.

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Our team

We are ten master students studying molecular biotechnology, genome-based system biology and molecular cell biology. Curious who we are? Get to know us better!

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Human practices projects

A project is not only lab work. As scientists we need to take responsiblity of what we published and how we appear in public. Among other things we did a dual use essay as we want iGEM to be more aware of biosecurity. Read how iGEM can be a role model for dual use issues.

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Modeling

Biosafety

Our systems should be cell-free. We confirmed through experiments and experts that our systems contained no genetically modified organisms and are safe to use. Let's see what we've done.

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Notebook

We documented everything we have done in the laboratory and lists protocols, organisms, buffers and primers. If you need more information, check this page.

Partners

An important aspect of iGEM is collaboration. We collaborated with several iGEM teams and helped them for their project. Moreover, you can see who supports us financially. Take a look!

Achievements

Development of a new cell-free assay named Plasmid Repressor Interaction Assay (PRIA)
Establishment of a cell-free protein synthesis (CFPS) system for the iGEM community
Demonstration of the system's extensibility through several biosensors
Construction of a new biosensor for the detection of date rape drugs
Construction of a new biosensor for heavy metals (Copper) .
Implementation of a model for CFPS.
Taking up the dual use issue in connection with the topic "date rape drugs".
Help another team
We have a functional prototype.