Team:Paris Saclay/Notebook/August/27
Tuesday 27th August
Lab Work
Electrophoresis
by Audrey
Verification of PCR products:
- BBa_K1707013
- BBa_K1707019
- BBa_K1707020
- BBa_K1707030
- BBa_K1707035
- BBa_K1707036
- BBa_K1707021
- BBa_K1707027
- Controls (-)
Agarose gel 1%, Migration 115V
We can conclude that:
- BBa_K1707035 and BBa_K1707036 aren't OK, we will have to start again
All of the next one (+DMSO) are OK:
- BBa_K1707013
- BBa_K1707019
- BBa_K1707020
- BBa_K1707030
- BBa_K1707021
- BBa_K1707027
Digestion
by Audrey
PCR products of: (+DMSO)
- BBa_K1707013
- BBa_K1707019
- BBa_K1707020
- BBa_K1707030
- BBa_K1707021
- BBa_K1707027
- BBa_K1707036
Mix in each tube:
- 1 μL DpnI
- 45μL PCR Product
- 5,5 μL FastDigest Buffer
- 3,5 μL H2O
Incubation 1h30, 37°C
Digestion
by Audrey
- BBa_K1707022
Mix:
- 1 μL XbaI
- 1 μL PstI
- 15 μL plasmid
- 2 μL FastDigest Buffer
- 1 μL H2O
- BBa_K1707023
Mix:
- 1 μL SpeI
- 1 μL PstI
- 15 μL Plasmid
- 2 μL FastDigest Buffer
- 1 μL H2O
Incubation 1h30, 37°C
Member present:
- Instructors: Claire
- Students: Audrey
Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. *This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.