Team:NRP-UEA-Norwich/Parts
Parts
Part name | Backbone | Description | Function | Part number |
---|---|---|---|---|
Plac-GlgX | pSB1C3 | Debranching enzyme with IPTG inducible Promoter | Removes E. coli glycogen branches | BBa_K1618025 |
GlgX | pSB1C3 | Debranching enzyme | BBa_K1618026 | |
Plac-GlgB | pSB1C3 | Branching enzyme with IPTG inducible Promoter | Add E. coli glycogen branches | BBa_K1618022 |
GlgB | pSB1C3 | Branching Enzyme | BBa_K1618000 | |
Plac-MAO | pSB1C3 | Acylation candidate 1 with IPTG inducible Promoter | Acylates glycogen? (1) | BBa_K1618005 |
MAO | pSB1C3 | Acylation Candidate 1 | BBa_K1618001 | |
Plac-Rv3030 | pSB1C3 | Acylation candidate 2 with IPTG inducible Promoter | Acylates glycogen? (2) | BBa_K1618007 |
Rv3030 | pSB1C3 | Acylation candidate 2 | BBa_K1618002 | |
Plac-Rv3034c | pSB1C3 | Acylation candidate 3 with IPTG inducible Promoter | Acylates glycogen? (3) | BBa_K1618008 |
Rv3034c | pSB1C3 | Acylation candidate 3 | BBa_K1618003 | |
Plac-Rv3037c | pSB1C3 | Acylation candidate 4 with IPTG inducible Promoter | Acylates glycogen? (4) | BBa_K1618009 |
Rv3037c | pSB1C3 | Acylation candidate 4 | BBa_K1618004 | |
Plac-GlgB-Rv3030 | pSB1C3 | Branching enzyme + Acylation candidate 2 with IPTG inducible Promoter | Makes acetylated branched glycogen? (2) | BBa_K1618023 |
Plac-GlgB-Rv3034c | pSB1C3 | Branching enzyme + Acylation candidate 3 with IPTG inducible Promoter | Makes acetylated branched glycogen? (3) | BBa_K1618024 |
Plac-GlgB-Rv3037c | pSB1C3 | Branching enzyme + Acylation candidate 4 with IPTG inducible Promoter | Makes acetylated branched glycogen? (4) | BBa_K1618027 |
Plac-Buk | pSB1C3 | Butyrate kinase with IPTG inducible Promoter | Reversible reaction that makes butyrate from butyryl phosphate | BBa_K1618012 |
Plac-PTB | pSB1C3 | Phosphotransbutrylase with IPTG inducible Promoter | Reversible reaction that makes butyryl-phosphate from butyryl-CoA | BBa_K1618013 |
Plac-THL | pSB1C3 | Thiolase with IPTG inducible Promoter | Makes Acetoacetyl-CoA from Acetyl-CoA | BBa_K1618014 |
Plac-CRO | pSB1C3 | Crotonase with IPTG inducible Promoter | Makes Crotonyl-CoA from 3-hydroxybutyryl-CoA | BBa_K1618015 |
Plac-BHBD | pSB1C3 | β-hydroxybutyryl-CoA dehydrogenase with IPTG inducible Promoter | Makes 3-hydroxybutyryl-CoA from Acetoacetyl-CoA | BBa_K1618016 |
Plac-BCD | pSB1C3 | Butyryl-CoA dehydrogenase with IPTG inducible Promoter | Makes Butyryl-CoA from Crotonyl-CoA | BBa_K1618017 |
Plac-EFTb | pSB1C3 | Electron transfer protein β with IPTG inducible Promoter | Makes butyrate from acetate part 5 | BBa_K1618018 |
Plac-EFTa | pSB1C3 | Electron transfer protein α with IPTG inducible Promoter | Makes butyrate from acetate part 6 | BBa_K1618019 |
Plac-Buk-PTB | pSB1C3 | Butyrate to butyryl CoA pathway with IPTG inducible Promoter | Reversible reaction that makes Butyrate from Butyryl-CoA | BBa_K1618020 |
Plac-THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl-CoA to Butyrate pathway with IPTG inducible Promoter | Makes butyrate from Acetyl-CoA full | BBa_K1618021 |
35S-MAO | ? | Acylation candidate 1 with 35S Promoter | Acetylates starch? (1) | BBa_K1618033 |
35S-Rv3030 | ? | Acylation candidate 2 with 35S Promoter | Acetylates starch? (2) | BBa_K1618034 |
35S-Rv3034c | ? | Acylation candidate 3 with 35S Promoter | Acetylates starch? (3) | BBa_K1618035 |
35S-Rv3037c | ? | Acylation candidate 4 with 35S Promoter | Acetylates starch? (4) | BBa_K1618036 |
35S-GFP-MAO | ? | Acylation candidate 1 with 35S Promoter + GFP | Acetylates starch? (1) + GFP tag | BBa_K1618029 |
35S-GFP-Rv3030 | ? | Acylation candidate 2 with 35S Promoter + GFP | Acetylates starch? (2) + GFP tag | BBa_K1618030 |
35S-GFP-Rv3034c | ? | Acylation candidate 3 with 35S Promoter + GFP | Acetylates starch? (3) + GFP tag | BBa_K1618031 |
35S-GFP-Rv3037c | ? | Acylation candidate 4 with 35S Promoter + GFP | Acetylates starch? (4) + GFP tag | BBa_K1618032 |
Chloroplast Transit Peptide | ? | Chloroplast Transit Peptide | BBa_K1618028 | |
35s Terminator | ? | Terminator | BBa_K1618037 |