Team:NRP-UEA-Norwich/Parts

House of Carbs

Parts

Part name Backbone Description Function Part number
Plac-GlgXpSB1C3Debranching enzyme with IPTG inducible PromoterRemoves E. coli glycogen branchesBBa_K1618025
GlgXpSB1C3Debranching enzyme  BBa_K1618026
Plac-GlgBpSB1C3Branching enzyme with IPTG inducible PromoterAdd E. coli glycogen branchesBBa_K1618022
GlgBpSB1C3Branching Enzyme  BBa_K1618000
Plac-MAOpSB1C3Acylation candidate 1 with IPTG inducible PromoterAcylates glycogen? (1)BBa_K1618005
MAOpSB1C3Acylation Candidate 1  BBa_K1618001
Plac-Rv3030pSB1C3Acylation candidate 2 with IPTG inducible PromoterAcylates glycogen? (2)BBa_K1618007
Rv3030pSB1C3Acylation candidate 2 BBa_K1618002
Plac-Rv3034cpSB1C3Acylation candidate 3 with IPTG inducible PromoterAcylates glycogen? (3) BBa_K1618008
Rv3034cpSB1C3Acylation candidate 3  BBa_K1618003
Plac-Rv3037cpSB1C3Acylation candidate 4 with IPTG inducible PromoterAcylates glycogen? (4) BBa_K1618009
Rv3037cpSB1C3Acylation candidate 4  BBa_K1618004
Plac-GlgB-Rv3030pSB1C3Branching enzyme + Acylation candidate 2 with IPTG inducible PromoterMakes acetylated branched glycogen? (2)BBa_K1618023
Plac-GlgB-Rv3034cpSB1C3Branching enzyme + Acylation candidate 3 with IPTG inducible PromoterMakes acetylated branched glycogen? (3)BBa_K1618024
Plac-GlgB-Rv3037cpSB1C3Branching enzyme + Acylation candidate 4 with IPTG inducible PromoterMakes acetylated branched glycogen? (4)BBa_K1618027
Plac-BukpSB1C3Butyrate kinase with IPTG inducible PromoterReversible reaction that makes butyrate from butyryl phosphateBBa_K1618012
Plac-PTBpSB1C3Phosphotransbutrylase with IPTG inducible PromoterReversible reaction that makes butyryl-phosphate from butyryl-CoABBa_K1618013
Plac-THLpSB1C3Thiolase with IPTG inducible PromoterMakes Acetoacetyl-CoA from Acetyl-CoABBa_K1618014
Plac-CROpSB1C3Crotonase with IPTG inducible PromoterMakes Crotonyl-CoA from 3-hydroxybutyryl-CoABBa_K1618015
Plac-BHBDpSB1C3β-hydroxybutyryl-CoA dehydrogenase with IPTG inducible PromoterMakes 3-hydroxybutyryl-CoA from Acetoacetyl-CoA BBa_K1618016
Plac-BCDpSB1C3Butyryl-CoA dehydrogenase with IPTG inducible PromoterMakes Butyryl-CoA from Crotonyl-CoABBa_K1618017
Plac-EFTbpSB1C3Electron transfer protein β with IPTG inducible PromoterMakes butyrate from acetate part 5BBa_K1618018
Plac-EFTapSB1C3Electron transfer protein α with IPTG inducible PromoterMakes butyrate from acetate part 6BBa_K1618019
Plac-Buk-PTBpSB1C3Butyrate to butyryl CoA pathway with IPTG inducible Promoter Reversible reaction that makes Butyrate from Butyryl-CoABBa_K1618020
Plac-THL-CRO-BHBD-BCD-EFTb-EFTapSB1C3Acetyl-CoA to Butyrate pathway with IPTG inducible Promoter Makes butyrate from Acetyl-CoA fullBBa_K1618021
35S-MAO?Acylation candidate 1 with 35S PromoterAcetylates starch? (1)BBa_K1618033
35S-Rv3030?Acylation candidate 2 with 35S PromoterAcetylates starch? (2)BBa_K1618034
35S-Rv3034c?Acylation candidate 3 with 35S PromoterAcetylates starch? (3)BBa_K1618035
35S-Rv3037c?Acylation candidate 4 with 35S PromoterAcetylates starch? (4)BBa_K1618036
35S-GFP-MAO?Acylation candidate 1 with 35S Promoter + GFPAcetylates starch? (1) + GFP tagBBa_K1618029
35S-GFP-Rv3030?Acylation candidate 2 with 35S Promoter + GFPAcetylates starch? (2) + GFP tagBBa_K1618030
35S-GFP-Rv3034c?Acylation candidate 3 with 35S Promoter + GFPAcetylates starch? (3) + GFP tagBBa_K1618031
35S-GFP-Rv3037c?Acylation candidate 4 with 35S Promoter + GFPAcetylates starch? (4) + GFP tagBBa_K1618032
Chloroplast Transit Peptide? Chloroplast Transit PeptideBBa_K1618028
35s Terminator? TerminatorBBa_K1618037

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