Team:HKUST-Rice/Expression/ParaBAD

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P_araBAD characterization

Parallel Sensor

Application

Investigation on P_araBAD

P_araBAD is a widely used promoter, its BioBrick, BBa_I0500, reported 392 uses to date (as of 4th Sep 2015). Yet different behaviors of P_araBAD have been reported. Cambridge 2011 iGEM team reported an All-or-None behavior whereas Groningen 2011 iGEM team reported a graded response.

We found that P_araBAD did not display all-or-none behavior on a single cell level, which is in disagreement with Cambridge 2011 iGEM team. Also, we shows that on plasmid with different copy number, P_araBAD is sensitive to different range of arabinose.

Since this year our team will also use this promoter in the project, we wish to further characterize this promoter. Through this investigation, our team hope to caution users in choosing this promoter for future construction.

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