Team:Penn/Sender

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PENN iGEM 2015

SENDER

The first component of our project focused on will be used to determine if the light produced by the lux box is sufficient to activate the light-activated transcription factor. This experiment requires a new component to the aforementioned genetic circuit in pDawn. The new component is initiated by a constitutive promoter (BBa_J23100) and followed by an RBS + lux box. Expression of the genes in the lux box is responsible for producing luminescence. The box contains the following genes in order: LuxC, D, A, B, E and G. LuxA and B are responsible for encoding two subunits of bacterial luciferase. The genes LuxC, D, and E code for the substrate for the light emitting reaction, tetradecanal. The function of the luxG gene is yet to be fully elucidated; however, inclusion of the gene is known to increase light output. The circuit is completed with a stop codon and a terminator sequence.

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