Team:MIT/Coculture
Project
Coculture
Preliminary Coculture
Methods for Naive Coculture
The preliminary co-culture experiment served to determine how unmodified C. hutchinsonii and E. coli grow in mono-cultures versus co-cultures over time. Five cultures with co-culture media, filter paper as a carbon source, and variations of E. coli and C. hutchinsonii populations were prepared, with replicates of each, as shown in Table 1. The conditioned media was co-culture media that had contained filter paper and C. hutchinsonii for three days. The cultures were incubated in a 30ºC incubator shaking at 250 rpm. Samples were taken before incubation and then every 3 hours for the first 12 hours and once every 24 hours on days 4-8. Cells from each sample were isolated from the samples and the supernatant via the co-culture protocol. We use the flow cytometer to measure the size of cell populations from the glycerol stocks of the cells, as detailed in Measuring Relative Populationed.
Table 1
