Team:UST Beijing
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Individual Variation of RFP accumulation in E.coli
AGE-RELATED PHENOMENA: A HYPOTHESIS
We know that E.coli divides in LB medium at 37C around 20-30min per cycle. In this 20-30 minutes, the bacterium has to duplicate its DNA, 4.5 million base pairs, produce every protein of its body another copy, assemble them into structures, and divide into two almost identical copies of individual bacterium.
On top of this, if we introduce a plasmid of 3kb long, 500 copies of it, into every E.coli individual, we would add some pressure to this replicating process. In this plasmid, a RFP protein expression cassette (part number J022150?) is inserted. The promoter has a lacI operon but in DH5alpha strain, its basal expression seems not under tight LacI control, probably due to weak expression levels of LacI in DH5alpha, and its huge copy numbers.
Once upon a time, there was a group of lovely red fluorescent protein plasmid. One day they met another very lovely group of e. coli, and they play together happily. China has an old saying: He who gets in contact with vermilion will become red. The e. coli who Gets along well with red fluorescent protein plasmid were dyed red. But some red fluorescent protein plasmid and some e. coli are shy, this part of e. coli is not dyed red. What happened between them? Let's explore their story~
What can be done with One yuan? In numerous answers, we choose to use it to make an RFP quantitative determination device.
We used one yuan to buy the diode and some material, and assemble them into the simple device which present in the video.At first By using the hand crank, the current is not uniform, then we on the basis of the device installed on a little secret, so it can generate steady current ~
Compared with large measuring protein device, the quantitative measurement of RFP device is more compact. One day in the field you hit upon an idea want to measure the red fluorescent protein content, use it!
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University of Science and Technology Beijing No.30, Xueyuan road, Haidian district, Beijing Province, China
Beijing City University No.269, Middle North 4th Ring Road, Haidian district, Beijing Province, China
