Team:NCTU Formosa/Safe Project Design

Safe Project Design

Choosing a non-pathogenic chassis

Bacterial strain: Escherichia coli K12

Escherichia coli K-12 are not a threat to human, animal (chickens, pigs, and calves), plants or the environment, according to the 1997 Final Risk Assessment of Environmental Protection Agency (EPA). “Any concerns in terms of health considerations are mitigated by its poor ability to disseminate, colonize the colon and establish infections in a murine model” (Smith et al., 2010). In addition, the chance of the insertion gene mutating the bacterial strain to be hazardous is minimal as the inserting genetic materials have to meet the EPA’s criteria (limited in size, well characterized, free of certain nucleotide sequences, and poor mobility).

Choosing parts that will not harm humans/ animals/ plants

NameNatural FunctionAcquired ByNotes
Cell lines
H292
(Mucoepidermoid Pulmonary Carcinoma cell)		CellCompany
HCC827 (Adenocarcinoma)CellCompany
H1975 (Adenocarcinoma)CellCompany
MCF7 (Adenocarcinoma)CellCompany
MDA-MB-231(Adenocarcinoma)CellCompany
SK-BR-3 (Adenocarcinoma)CellCompany
Normal Backbone
pSB1A3Plasmid backboneKithttp://parts.igem.org/Part:pSB1A3
pSB1K3Plasmid backboneKithttp://parts.igem.org/Part:pSB1K3
pSB1C3Plasmid backboneKithttp://parts.igem.org/Part:pSB1C3
Expression Backbone
pSB6A1Plasmid backboneKithttp://parts.igem.org/Part:pSB6A1

Inserts:



Back to Lab Safety

NameNatural FunctionAcquired ByNotes
Promoters
J23101Consitutive promoterKithttp://parts.igem.org/Part:BBa_J23101
J23110Consitutive promoterKithttp://parts.igem.org/Part:BBa_J23110
R0010Induced promoterKithttp://parts.igem.org/Part:BBa_R0010
Ribosome Binding Site (RBS)
B0034Strong; efficiency 1.0Kithttp://parts.igem.org/Part:BBa_B0034
B0030Strong; efficiency 0.91Kithttp://parts.igem.org/Part:BBa_B0032