Template:SJTU-BioX-Shanghai/Notebook/Transformation/w8
Contents
Transformation
pdark natural transformation
In the evening of Aug. 29
2nd liquid segregation of pdark-HR
pcpcG2 electro-transformation
In the evening of Aug. 24
We picked 4 colonies (marked with 1,2,3 & 4) from the pcpcG2-HR plate and spread them on another BG11 plate in parallel seprately to do solid segregation
In the evening of Aug. 30
We scraped the grown cell from the plate, which is 2,3 & 4 to do colony PCR, 1st liquid segregation and solid storage
pre-experiment of pdark-HR
on 24th, Aug
Took the third 4 samples at 9am & fourth 4 samples at 9pm.
on 25th, Aug
Took the fifth 4 samples at 9am & sixth 4 samples at 9pm.
on 26th, Aug
Took the seventh 4 samples at 9am & eighth 4 samples at 9pm.
pdark-GFP electro-transformation
in the evening of 24th, Aug
Set culture.
in the evening of 26th, Aug
Electro-transformation of pdark-GFP.
in the evening of 27th, Aug
Collected half of the cell and mixed with 5ml soft agar, then poured on solid medium plate.